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Special Issue "Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides"

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A special issue of Marine Drugs (ISSN 1660-3397). This special issue belongs to the section "Structural Studies on Marine Natural Products".

Deadline for manuscript submissions: 29 February 2024 | Viewed by 4717

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Special Issue Editors

Prof. Dr. Lijun You

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Guest Editor

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
Interests: polysaccharides; hypoglycemic; anticancer; antioxidant; anti-UV radiation activities; functional food
Special Issues, Collections and Topics in MDPI journals

Dr. Saiyi Zhong

E-Mail Website
Guest Editor

Department of Food Science and Technology, Guangdong Ocean University, Zhanjiang, China
Interests: food-derive mutagens and carcinogens; anticancer food factors; bioactive chemopreventive food components
Special Issues, Collections and Topics in MDPI journals

Dr. Riming Huang

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Guest Editor

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, China
Interests: green directional creation and precise nutrition of marine biological polysaccharides/oligosaccharides

Special Issue Information

Dear Colleagues,

We are pleased to invite you to submit original articles to the Special Issue entitled “Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides”. The purpose of this Special Issue is to feature latest, high-quality, advanced research and knowledge contributed by various research groups all around the world working on polysaccharides and peptides from marine sources, which will shape future research directions.

Polysaccharides and peptides play a vital role in the areas of human wellness and food. They have been reported to present various physiological and biological activities based on their specific chemical structures. However, their structure–function relationships are yet to be established. Today, special attention has been paid to investigating their structure–function relationships and the mechanisms of their biological activities, which facilitate their being used as food ingredients or bioactive compounds in the food and pharmaceutical fields.

We would like to invite scientists to participate in this Special Issue by submitting an updated and new base of knowledge on the isolation, identification and applications of marine source polysaccharides and peptides.

Potential topics include, but are not limited to, the following:

Isolation and structural identification of marine-source polysaccharides or peptides
Effects of processing methods on the functions of marine source polysaccharides or peptides.
Functions of marine-source polysaccharides or peptides, in vitro or in vivo evaluation
Industrial and potential applications of marine-source polysaccharides or peptides.
Product research and development of marine-source polysaccharides or peptides.

Prof. Dr. Lijun You
Dr. Saiyi Zhong
Dr. Riming Huang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Marine Drugs is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

isolation
structure
polysaccharide
peptide
identification
biological activity

Published Papers (6 papers)

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Research

11 pages, 2493 KiB

Open AccessArticle

UV/H₂O₂-Degraded Polysaccharides from Sargassum fusiforme: Purification, Structural Properties, and Anti-Inflammatory Activity

and

Mar. Drugs 2023, 21(11), 561; https://doi.org/10.3390/md21110561 - 26 Oct 2023

Viewed by 612

Abstract

The main purpose of this study was to analyze the structural properties and anti-inflammatory activity of the purified fractions derived from UV/H₂O₂-degraded polysaccharides from Sargassum fusiforme. Results indicated that twofractions with different monosaccharide compositions and morphological characteristics, PT-0.25 (yield 39.5%) and PT-0.5 (yield 23.9%), were obtained. The average molecular weights of PT-0.25 and PT-0.5 were 14.52 kDa and 22.89 kDa, respectively. In addition, PT-0.5 exhibited better anti-inflammatory activity with a clear dose dependence. The mechanism was associated with the inhibition of LPS-activated Toll-like receptor 4-mediated inflammatory pathways in RAW264.7 cells. The results showed that PT-0.5 was a complex polysaccharide mainly composed of 4-Fucp, t-Manp, 6-Galp, t-Fucp, and 3,4-GlcAp. These results would provide theoretical support for studying the structural properties and biological activities of UV/H₂O₂-degraded polysaccharides. Full article

(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)

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14 pages, 1917 KiB

Open AccessArticle

Preparation and Characterization of an Oyster Peptide–Zinc Complex and Its Antiproliferative Activity on HepG₂ Cells

Bo Peng

Zhu Chen

and

Yejia Wang

Mar. Drugs 2023, 21(10), 542; https://doi.org/10.3390/md21100542 - 18 Oct 2023

Viewed by 729

Abstract

It is evident that zinc supplementation is essential for maintaining good health and preventing disease. In this study, a novel oyster peptide–zinc complex with an average molecular weight of 500 Da was prepared from oyster meat and purified using ultrafiltration, ultrasound, a programmed cooling procedure, chelating, and dialysis. The optimal chelating process parameters obtained through a response surface methodology optimization design are a peptide/zinc ratio of 15, pH of 6.53, reaction time of 80 min, and peptide concentration of 0.06 g/mL. Then, the structure of a peptide–zinc complex (named COP2-Zn) was investigated using the UV and infrared spectrums. The results showed that the maximum absorption peak was redshifted from 224.5 nm to 228.3 nm and the main difference of the absorption peaks was 1396.4 cm⁻¹. The cytotoxicity and antiproliferative effects of COP2-Zn were evaluated. The results showed that COP2-Zn had a better antiproliferative effect than the unchelated peptide against HepG₂ cells. A DNA flow cytometric analysis showed that COP2-Zn induced S-phase arrest in HepG₂ cells in a dose-dependent manner. Additionally, the flow cytometer indicated that COP2-Zn significantly induced HepG₂ cell apoptosis. Full article

(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)

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12 pages, 3074 KiB

Open AccessArticle

The Preventive Mechanism of Anserine on Tert-Butyl Hydroperoxide-Induced Liver Injury in L-02 Cells via Regulating the Keap1-Nrf2 and JNK-Caspase-3 Signaling Pathways

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Mar. Drugs 2023, 21(9), 477; https://doi.org/10.3390/md21090477 - 29 Aug 2023

Viewed by 730

Abstract

Anserine is a naturally occurring histidine dipeptide with significant antioxidant activities. This study aimed to investigate the preventive mechanism of anserine on tert-butyl hydroperoxide (TBHP)-induced liver damage in a normal human liver cell line (L-02 cells). The L-02 cells were pretreated with anserine (10, 20, and 40 mmol/L) and then induced with 400 μmol/L of TBHP for 4 h. The results showed that the survival rates of L-02 cells and the contents of GSH were significantly increased with the pretreatment of anserine; the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the extracellular fluid were sharply decreased; and the formation of reactive oxygen species (ROS), nuclear fragmentation, and apoptosis were significantly inhibited. In addition, anserine could bind to the Kelch domain of Kelch-like ECH-associated protein 1 (Keap1) with a binding force of −7.2 kcal/mol; the protein expressions of nuclear factor-erythroid 2-related factor-2 (Nrf2), quinone oxidoreductase 1 (NQO1), heme oxygenase-1 (HO-1), and Bcl-2 were upregulated by anserine in TBHP-induced L-02 cells, with the downregulation of p-JNK and caspase-3. In conclusion, anserine might alleviated liver injury in L-02 cells via regulating related proteins in the Keap1-Nrf2 and JNK-Caspase-3 signaling pathways. Full article

(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)

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20 pages, 4452 KiB

Open AccessArticle

Enhanced In Vitro Anti-Photoaging Effect of Degraded Seaweed Polysaccharides by UV/H₂O₂ Treatment

Peter Chi-Keung Cheung

and

Viktoryia I. Kulikouskaya

Mar. Drugs 2023, 21(8), 430; https://doi.org/10.3390/md21080430 - 29 Jul 2023

Cited by 1 | Viewed by 867

Abstract

The high molecular weight and poor solubility of seaweed polysaccharides have limited their function and application. In this study, ultraviolet/hydrogen peroxide (UV/H₂O₂) treatment was used to prepare low-molecular-weight seaweed polysaccharides from Sargassum fusiforme. The effects of UV/H₂O₂ treatment on the physicochemical properties and anti-photoaging activity of S. fusiforme polysaccharides were studied. UV/H₂O₂ treatment effectively degraded polysaccharides from S. fusiforme (DSFPs), reducing their molecular weight from 271 kDa to 26 kDa after 2 h treatment. The treatment did not affect the functional groups in DSFPs but changed their molar percentage of monosaccharide composition and morphology. The effects of the treatment on the anti-photoaging function of S. fusiforme polysaccharides were investigated using human epidermal HaCaT cells in vitro. DFSPs significantly improved the cell viability and hydroxyproline secretion of UVB-irradiated HaCaT cells. In particular, DSFP-45 obtained from UV/H₂O₂ treatment for 45 min showed the best anti-photoaging effect. Moreover, DSFP-45 significantly increased the content and expression of collagen I and decreased those of pro-inflammatory cytokines, including interleukin-1β, interleukin-6, and tumor necrosis factor-α. Thus, UV/H₂O₂ treatment could effectively improve the anti-photoaging activity of S. fusiforme polysaccharides. These results provide some insights for developing novel and efficient anti-photoaging drugs or functional foods from seaweed polysaccharides. Full article

(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)

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15 pages, 2211 KiB

Open AccessArticle

Identification, Screening and Antibacterial Mechanism Analysis of Novel Antimicrobial Peptides from Sturgeon (Acipenser ruthenus) Spermary

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Mar. Drugs 2023, 21(7), 386; https://doi.org/10.3390/md21070386 - 29 Jun 2023

Cited by 1 | Viewed by 1013

Abstract

Fish is an important source of antimicrobial peptides. This study aimed to identify and screen antibacterial peptides with excellent antibacterial activity derived from sturgeon spermary peptides (SSPs) and to analyze their antibacterial activity and mechanism. Liquid chromatography-mass spectrometry/mass spectrometry methods were used to analyze and identify peptide sequences, computational prediction tool and molecular docking methods were used for virtual screening of antimicrobial peptides, and finally, candidate peptides were synthesized by solid-phase synthesis method. The results demonstrate that SSPs have excellent inhibitory activity against Escherichia coli with an inhibitory rate of 76.46%. Most parts of the SSPs were derived from the sturgeon (Acipenser ruthenus) histones, and the coverage of histone H2B was the highest (45%). Two novel peptides (NDEELNKLM and RSSKRRQ) were obtained by in silico prediction tools and molecular docking, which may interact with the DNA gyrase and dihydrofolate reductase of E. coli by forming salt bridges and hydrogen bonds. Compared to the individual peptides, the antibacterial effect was significantly improved by mixing the two peptides in equal proportions. Two novel peptides change the permeability of the E. coli cell membranes and may exert antimicrobial activity by inhibiting the metabolic process of the nucleic acids. Full article

(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)

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12 pages, 2974 KiB

Open AccessArticle

Extraction, Characterization and Osteogenic Activity of a Type I Collagen from Starfish (Asterias amurensis)

and

Mar. Drugs 2023, 21(5), 274; https://doi.org/10.3390/md21050274 - 27 Apr 2023

Cited by 4 | Viewed by 1507

Abstract

Outbreaks of starfish (Asterias amurensis) pose a major threat to aquaculture and marine ecosystems in Qingdao, China, and no effective methods have been found to control them. A comprehensive study of collagen in starfish could be an alternative to high efficient utilization. Based on this, collagen was firstly extracted from Qingdao A. amurensis. Then, its protein pattern, amino acid composition, secondary structure, microstructure and thermal stability were investigated. The results showed that the A. amurensis collagen (AAC) is a type I collagen composed of α₁, α₂, and β chains. Glycine, hydroxyproline, and alanine were the major amino acids. The melting temperature was 57.7 °C. From FTIR, UV spectra and CD chromatography, the AAC had an intact triple helix and secondary structure, and microstructural analysis showed that the AAC had a loose, fibrous porous structure. Next, the osteogenic differentiation effect of AAC on Mouse bone marrow stem cells (BMSCs) was investigated, and the results showed that AAC induced osteogenic differentiation of cells by promoting the proliferation of BMSCs, enhancing alkaline phosphatase (ALP) activity, promoting cell mineralization nodules and upregulating the expression of mRNA of relevant osteogenic genes. These results suggest that AAC might have the potential application to bone health-related functional foods. Full article

(This article belongs to the Special Issue Isolation, Identification and Applications of Marine Source Polysaccharides and Peptides)

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