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25 pages, 7242 KiB

Open AccessArticle

First Description of Non-Enzymatic Radical-Generating Mechanisms Adopted by Fomitiporia mediterranea: An Unexplored Pathway of the White Rot Agent of the Esca Complex of Diseases

by Samuele Moretti, Mary-Lorène Goddard, Alessandro Puca, Jacques Lalevée, Stefano Di Marco, Laura Mugnai, Eric Gelhaye, Barry Goodell, Christophe Bertsch and Sibylle Farine

J. Fungi 2023, 9(4), 498; https://doi.org/10.3390/jof9040498 - 21 Apr 2023

Viewed by 1923

Abstract

Fomitiporia mediterranea (Fmed) is the primary Basidiomycota species causing white rot in European vineyards affected by the Esca complex of diseases (ECD). In the last few years, an increasing number of studies have highlighted the importance of reconsidering the role of [...] Read more.

Fomitiporia mediterranea (Fmed) is the primary Basidiomycota species causing white rot in European vineyards affected by the Esca complex of diseases (ECD). In the last few years, an increasing number of studies have highlighted the importance of reconsidering the role of Fmed in ECD etiology, justifying an increase in research interest related to Fmed’s biomolecular pathogenetic mechanisms. In the context of the current re-evaluation of the binary distinction (brown vs. white rot) between biomolecular decay pathways induced by Basidiomycota species, our research aims to investigate the potential for non-enzymatic mechanisms adopted by Fmed, which is typically described as a white rot fungus. Our results demonstrate how, in liquid culture reproducing nutrient restriction conditions often found in wood, Fmed can produce low molecular weight compounds, the hallmark of the non-enzymatic “chelator-mediated Fenton” (CMF) reaction, originally described for brown rot fungi. CMF reactions can redox cycle with ferric iron, generating hydrogen peroxide and ferrous iron, necessary reactants leading to hydroxyl radical (^•OH) production. These observations led to the conclusion that a non-enzymatic radical-generating CMF-like mechanism may be utilized by Fmed, potentially together with an enzymatic pool, to contribute to degrading wood constituents; moreover, indicating significant variability between strains. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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18 pages, 7318 KiB

Open AccessArticle

Fungal Pathogens Associated with Strawberry Crown Rot Disease in China

by Yanting Zhang, Hong Yu, Meihua Hu, Jianyan Wu and Chuanqing Zhang

J. Fungi 2022, 8(11), 1161; https://doi.org/10.3390/jof8111161 - 02 Nov 2022

Cited by 9 | Viewed by 2743

Abstract

Strawberry crown rot (SCR) is a serious disease that is generally referred to as seedling anthracnose due to its association with Colletotrichum spp. Presently, SCR is the main cause of death of strawberry seedlings. However, management strategies, including fungicides targeting Colletotrichum spp., have [...] Read more.

Strawberry crown rot (SCR) is a serious disease that is generally referred to as seedling anthracnose due to its association with Colletotrichum spp. Presently, SCR is the main cause of death of strawberry seedlings. However, management strategies, including fungicides targeting Colletotrichum spp., have failed to obtain satisfactory results. Therefore, identifying the exact pathogen species causing SCR could guide its management. A total of 287 isolates were obtained from SCR-diseased plants. Based on the culture, morphology, and phylogenetic characteristics, the above 287 fungal isolates of SCR pathogens were identified as 12 different species, including Colletotrichum siamense, C. fructicola, Fusarium oxysporum, F. commune, F. equiseti, F. solani, F. tricinctum, Epicoccum sorghinum, Stemphylium lycopersici, Clonostachys rosea, Phoma herbarum, and Curvularia trifolii. Pathogenicity results showed that most isolates were pathogenic to strawberry seedlings and exhibited different degrees of virulence. In severe cases, poor growth on the ground, yellowing of the leaves, and even death of seedlings occurred. In mild cases, only black disease spots appeared on the stems of the strawberry seedlings, and a few withered leaves became necrotic. The inoculation experiments showed that the most virulent species were C. siamense and F. oxysporum, followed by F. equiseti, P. herbarum, Cl. rosea, S. lycopersici, and C. fructicola, which had disease incidences above 50%. E. sorghinum, S. lycopersici, Cl. rosea, P. herbarum and Cu. trifolii were reported to cause SCR for the first time herein. In conclusion, SCR is a sophisticated disease caused by a diversity of pathogenic fungi. This work provides new valuable data about the diversity and pathogenicity of SCR pathogens, which will help in formulating effective strategies to better control of the SCR disease. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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26 pages, 742 KiB

Open AccessArticle

The Role of Preharvest Natural Infection and Toxin Contamination in Food and Feed Safety in Maize, South-East Hungary, 2014–2021

by Akos Mesterhazy, Denes Szieberth, Eva Toldine Tóth, Zoltan Nagy, Balazs Szabó, Beata Herczig, Istvan Bors and Beata Tóth

J. Fungi 2022, 8(10), 1104; https://doi.org/10.3390/jof8101104 - 19 Oct 2022

Cited by 1 | Viewed by 2010

Abstract

Mycotoxins originating in the preharvest period represent a less studied research problem, even though they are of the utmost practical significance in maize production, determining marketability (within EU limits), and storage ability, competitiveness, and profit rate. In this study, 18–23 commercial hybrids were [...] Read more.

Mycotoxins originating in the preharvest period represent a less studied research problem, even though they are of the utmost practical significance in maize production, determining marketability (within EU limits), and storage ability, competitiveness, and profit rate. In this study, 18–23 commercial hybrids were tested between 2014 and 2021. Natural infection from Fusarium spp. was higher than 1.5%, and for Aspergillus spp. this was normally 0.01% or 0, much lower than would be considered as severe infection. In spite of this, many hybrids provided far higher toxin contamination than regulations allow. The maximum preharvest aflatoxin B₁ was in 2020 (at 2286 μg/kg), and, in several cases, the value was higher than 1000 μg/kg. The hybrid differences were large. In Hungary, the presence of field-originated aflatoxin B₁ was continuous, with three AFB1 epidemics in the 8 years. The highest DON contamination was in 2014 (at 27 mg/kg), and a detectable DON level was found in every hybrid. FUMB₁+B₂ were the highest in 2014 (at 45.78 mg/kg). At these low infection levels, correlations between visual symptoms and toxin contaminations were mostly non-significant, so it is not feasible to draw a conclusion about toxin contamination from ear rot coverage alone. The toxin contamination of hybrids for a percentage of visual infection is highly variable, and only toxin data can decide about food safety. Hybrids with no visual symptoms and high AFB1 contamination were also identified. Preharvest control, including breeding and variety registration, is therefore of the utmost importance to all three pathogens. Even natural ear rot and toxin data do not prove differences in resistance, so a high ear rot or toxin contamination level should be considered as a risk factor for hybrids. The toxin control of freshly harvested grain is vital for separating healthy and contaminated lots. In addition, proper growing and storage conditions must be ensured to protect the feed safety of the grain. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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14 pages, 2879 KiB

Open AccessArticle

FgLEU1 Is Involved in Leucine Biosynthesis, Sexual Reproduction, and Full Virulence in Fusarium graminearum

by Shaohua Sun, Mingyu Wang, Chunjie Liu, Yilin Tao, Tian Wang, Yuancun Liang, Li Zhang and Jinfeng Yu

J. Fungi 2022, 8(10), 1090; https://doi.org/10.3390/jof8101090 - 17 Oct 2022

Cited by 3 | Viewed by 1601

Abstract

Fusarium head blight (FHB) caused by Fusarium graminearum is a significant disease among cereal crops. In F. graminearum, biosynthesis of leucine, which is a branched chain amino acid, is achieved by converting α-isopropylmalate to β-isopropylmalate catalyzed by isopropylmalate isomerase encoded by LEU1 [...] Read more.

Fusarium head blight (FHB) caused by Fusarium graminearum is a significant disease among cereal crops. In F. graminearum, biosynthesis of leucine, which is a branched chain amino acid, is achieved by converting α-isopropylmalate to β-isopropylmalate catalyzed by isopropylmalate isomerase encoded by LEU1. Considering the potential for targeting this pathway by fungicides, we characterized the gene FgLEU1 (FGSG-09589) in the Fusarium graminearum genome using bioinformatics methods. For functional characterization, we constructed a deletion mutant of FgLEU1 (ΔLEU1) through homologous recombination. Compared with the wild-type strain PH-1, ΔLEU1 showed slower colony growth and fewer aerial mycelia. Leucine addition was needed to ensure proper mutant growth. Further, ΔLEU1 showed decreased conidial production and germination rates, and could not produce ascospores. Moreover, ΔLEU1 showed complete loss of pathogenicity and reduced ability to produce deoxynivalenol (DON) and aurofusarin. Upstream and downstream genes of FgLEU1 were significantly upregulated in ΔLEU1. Contrary to previous reports, the deletion mutant was more resistant to osmotic stress and cell wall-damaging agents than the wild-type. Taken together, FgLEU1 plays a crucial role in leucine synthesis, aerial mycelial growth, sexual and asexual reproduction, pathogenicity, virulence, and pigmentation in Fusarium graminearum, indicating its potential as a target for novel antifungal agents. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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21 pages, 6478 KiB

Open AccessArticle

Sgh1, an SR-like Protein, Is Involved in Fungal Development, Plant Infection, and Pre-mRNA Processing in Fusarium graminearum

by Guanghui Wang, Peng Sun, Zhongjuan Sun, Jindong Zhu, Dan Yu, Zhe Tang, Zonghua Wang, Chenfang Wang and Huawei Zheng

J. Fungi 2022, 8(10), 1056; https://doi.org/10.3390/jof8101056 - 08 Oct 2022

Cited by 1 | Viewed by 1783

Abstract

Serine/arginine (SR) proteins are essential pre-mRNA splicing factors in eukaryotic organisms. Our previous studies have shownthat the unique SR-specific protein kinase Srk1 is important for RNA splicing and gene transcription in Fusarium graminearum, and interacts with two SR proteins, FgSrp1 and FgSrp2. [...] Read more.

Serine/arginine (SR) proteins are essential pre-mRNA splicing factors in eukaryotic organisms. Our previous studies have shownthat the unique SR-specific protein kinase Srk1 is important for RNA splicing and gene transcription in Fusarium graminearum, and interacts with two SR proteins, FgSrp1 and FgSrp2. In this study, we have identified an SR-like protein called Sgh1 in F. graminearum, which is orthologous to budding yeast paralogous Gbp2 and Hrb1. Our data have shownthat the Sgh1 is involved in vegetative growth, conidiation, sexual reproduction, DON synthesis, and plant infection. Moreover, the Sgh1 is mainly localized to the nucleus. RNA-seq analysis has shownthat the expression of over 1100 genes and the splicing efficiency in over 300 introns were affected in the Δsgh1 mutant. Although the RS domain and all three of the RRM domains are important for the Sgh1 functions, only the RS domain is responsible for its nuclear localization. Finally, we verified that the Sgh1 interacts with the unique SR-specific kinase Srk1 in F. graminearum by the yeast-two hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Taken together, our results have revealed that the Sgh1 regulates the fungal development, plant infection, and the pre-mRNA processing, and the RS domain regulates the function of the Sgh1 by modulating its nucleocytoplasmic shuttling. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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32 pages, 4766 KiB

Open AccessArticle

De Novo Long-Read Whole-Genome Assemblies and the Comparative Pan-Genome Analysis of Ascochyta Blight Pathogens Affecting Field Pea

by Yvonne O. Ogaji, Robert C. Lee, Tim I. Sawbridge, Benjamin G. Cocks, Hans D. Daetwyler and Sukhjiwan Kaur

J. Fungi 2022, 8(8), 884; https://doi.org/10.3390/jof8080884 - 22 Aug 2022

Viewed by 3360

Abstract

Ascochyta Blight (AB) is a major disease of many cool-season legumes globally. In field pea, three fungal pathogens have been identified to be responsible for this disease in Australia, namely Peyronellaea pinodes, Peyronellaea pinodella and Phoma koolunga. Limited genomic resources for [...] Read more.

Ascochyta Blight (AB) is a major disease of many cool-season legumes globally. In field pea, three fungal pathogens have been identified to be responsible for this disease in Australia, namely Peyronellaea pinodes, Peyronellaea pinodella and Phoma koolunga. Limited genomic resources for these pathogens have been generated, which has hampered the implementation of effective management strategies and breeding for resistant cultivars. Using Oxford Nanopore long-read sequencing, we report the first high-quality, fully annotated, near-chromosome-level nuclear and mitochondrial genome assemblies for 18 isolates from the Australian AB complex. Comparative genome analysis was performed to elucidate the differences and similarities between species and isolates using phylogenetic relationships and functional diversity. Our data indicated that P. pinodella and P. koolunga are heterothallic, while P. pinodes is homothallic. More homology and orthologous gene clusters are shared between P. pinodes and P. pinodella compared to P. koolunga. The analysis of the repetitive DNA content showed differences in the transposable repeat composition in the genomes and their expression in the transcriptomes. Significant repeat expansion in P. koolunga’s genome was seen, with strong repeat-induced point mutation (RIP) activity being evident. Phylogenetic analysis revealed that genetic diversity can be exploited for species marker development. This study provided the much-needed genetic resources and characterization of the AB species to further drive research in key areas such as disease epidemiology and host–pathogen interactions. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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16 pages, 3356 KiB

Open AccessArticle

Identification of Gene Modules and Hub Genes Associated with Sporisorium scitamineum Infection Using Weighted Gene Co-Expression Network Analysis

by Zongling Liu, Xiufang Li, Jie Li, Haiyun Zhao, Xingli Deng, Yizu Su, Ru Li and Baoshan Chen

J. Fungi 2022, 8(8), 852; https://doi.org/10.3390/jof8080852 - 15 Aug 2022

Cited by 2 | Viewed by 1604

Abstract

Sporisorium scitamineum is a biotrophic fungus responsible for sugarcane smut disease. To investigate the key genes involved in S. scitamineum infection, we conducted RNA sequencing of sugarcane sprouts inoculated with S. scitamineum teliospores. A weighted gene co-expression network analysis (WGCNA) showed that two [...] Read more.

Sporisorium scitamineum is a biotrophic fungus responsible for sugarcane smut disease. To investigate the key genes involved in S. scitamineum infection, we conducted RNA sequencing of sugarcane sprouts inoculated with S. scitamineum teliospores. A weighted gene co-expression network analysis (WGCNA) showed that two co-expressed gene modules, MEdarkturquoise and MEpurple—containing 66 and 208 genes, respectively—were associated with S. scitamineum infection. The genes in these two modules were further studied using Gene Ontology (GO) enrichment analysis, pathogen-host interaction (PHI) database BLASTp, and small secreted cysteine-rich proteins (SCRPs) prediction. The top ten hub genes in each module were identified using the Cytohubba plugin. The GO enrichment analysis found that endoplasmic reticulum-related and catabolism-related genes were expressed during S. scitamineum infection. A total of 83 genes had homologs in the PHI database, 62 of which correlated with pathogen virulence. A total of 21 proteins had the characteristics of small secreted cysteine-rich proteins (SCRPs), a common source of fungal effectors. The top ten hub genes in each module were identified, and seven were annotated as Mig1-Mig1 protein, glycosyl hydrolase, beta-N-acetylglucosaminidase, secreted chorismate mutase, collagen, mRNA export factor, and pleckstrin homology domain protein, while the remaining three were unknown. Two SCRPs—SPSC_06609 and SPSC_04676—and three proteins—SPSC_01958, SPSC_02155, and SPSC_00940—identified in the PHI database were also among the top ten hub genes in the MEdarkturquoise and MEpurple modules, suggesting that they may play important roles in S. scitamineum infection. A S. scitamineum infection model was postulated based on current findings. These findings help to deepen the current understanding of early events in S. scitamineum infection. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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16 pages, 1580 KiB

Open AccessArticle

Co-Occurrence Patterns of Ustilago nuda and Pyrenophora graminea and Fungicide Contribution to Yield Gain in Barley under Fluctuating Climatic Conditions in Serbia

by Radivoje Jevtić, Vesna Župunski, Mirjana Lalošević, Ljiljana Brbaklić and Branka Orbović

J. Fungi 2022, 8(5), 542; https://doi.org/10.3390/jof8050542 - 23 May 2022

Viewed by 1686

Abstract

The utilization of production systems with reduced chemical input renewed the interest in Ustilago nuda and Pyrenophora graminea. The investigations of seed fungicide treatments are more related to their efficacy than to their contribution to yield gain. The data were collected from [...] Read more.

The utilization of production systems with reduced chemical input renewed the interest in Ustilago nuda and Pyrenophora graminea. The investigations of seed fungicide treatments are more related to their efficacy than to their contribution to yield gain. The data were collected from research and development trials on fungicide efficacy against U. nuda and P. graminea conducted from 2014 to 2020 in Serbia. Partial least squares, multiple stepwise regression and best subset regression were used for statistical modeling. The total number of plants infected with U. nuda and P. graminea per plot differed significantly in the seven-year period. Shifts in the predominance of one pathogen over the other were also shown. Temperature, total rainfall and relative humidity in flowering time (p < 0.001) influenced the occurrence of both pathogens. The strongest impact on yield loss was observed for temperature in the phenological phases of leaf development (p = 0.014), temperature in flowering time (p < 0.001) and total number of plants infected with U. nuda and P. graminea per plot (p < 0.001). Our results indicated that regression models consisting of both biotic and abiotic factors were more precise in estimating regression coefficients. Neither fungicidal treatment had a stable contribution to yield gain in the seven-year period. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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23 pages, 2371 KiB

Open AccessArticle

Highly Diverse Phytophthora infestans Populations Infecting Potato Crops in Pskov Region, North-West Russia

by Eve Runno-Paurson, Collins A. Agho, Nadezda Zoteyeva, Mati Koppel, Merili Hansen, Tiit Hallikma, David E. L. Cooke, Helina Nassar and Ülo Niinemets

J. Fungi 2022, 8(5), 472; https://doi.org/10.3390/jof8050472 - 30 Apr 2022

Cited by 4 | Viewed by 2802

Abstract

There is limited understanding of the genetic variability in Phytophthora infestans in the major potato cultivation region of north-western Russia, where potato is grown primarily by small households with limited chemical treatment of late blight. In this study, the mating type, sensitivity to [...] Read more.

There is limited understanding of the genetic variability in Phytophthora infestans in the major potato cultivation region of north-western Russia, where potato is grown primarily by small households with limited chemical treatment of late blight. In this study, the mating type, sensitivity to metalaxyl, and genotype and population genetic diversity (based on 12 simple sequence repeat (SSR) markers) of 238 isolates of P. infestans from the Pskov region during the years 2010–2013 were characterized. The aim was to examine the population structure, phenotypic and genotypic diversity, and the prevalent reproductive mode of P. infestans, as well as the influence of the location, time, and agricultural management practices on the pathogen population. The frequency of the A2 mating was stable over the four seasons and ranged from 33 to 48% of the sampled population. Both mating types occurred simultaneously in 90% of studied fields, suggesting the presence of sexual reproduction and oospore production in P. infestans in the Pskov region. Metalaxyl-sensitive isolates prevailed in all four years (72%), however, significantly fewer sensitive isolates were found in samples from large-scale conventional fields. A total of 50 alleles were detected in the 141 P. infestans isolates analyzed for genetic diversity. Amongst the 83 SSR multilocus genotypes (MLGs) detected, 65% were unique and the number of MLGs varied between locations from 3 to 20. These results, together with the high genotypic diversity observed in all the locations and the lack of significance of linkage disequilibrium, suggest that sexual recombination is likely responsible for the unique MLGs and the high genetic diversity found in the Pskov region population, resembling those of north-eastern European populations. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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31 pages, 6567 KiB

Open AccessArticle

Updating the Methodology of Identifying Maize Hybrids Resistant to Ear Rot Pathogens and Their Toxins—Artificial Inoculation Tests for Kernel Resistance to Fusarium graminearum, F. verticillioides, and Aspergillus flavus

by Akos Mesterhazy, Denes Szieberth, Eva Tóth Toldine, Zoltan Nagy, Balázs Szabó, Beata Herczig, Istvan Bors and Beata Tóth

J. Fungi 2022, 8(3), 293; https://doi.org/10.3390/jof8030293 - 11 Mar 2022

Cited by 8 | Viewed by 2606

Abstract

Resistance to toxigenic fungi and their toxins in maize is a highly important research topic, as mean global losses are estimated at about 10% of the yield. Resistance and toxin data of the hybrids are mostly not given, so farmers are not informed [...] Read more.

Resistance to toxigenic fungi and their toxins in maize is a highly important research topic, as mean global losses are estimated at about 10% of the yield. Resistance and toxin data of the hybrids are mostly not given, so farmers are not informed about the food safety risks of their grown hybrids. According to the findings aflatoxin regularly occurs at preharvest in Hungary and possibly other countries in the region can be jeopardized. We tested, with an improved methodology (two isolates, three pathogens, and a toxin control), 18 commercial hybrids (2017–2020) for kernel resistance (%), and for toxin contamination separately by two–two isolates of F. graminearum, F. verticillioides (mg/kg), and A. flavus (μg/kg). The preharvest toxin contamination was measured in the controls. Highly significant kernel resistance and toxin content differences were identified between hybrids to the different fungi. Extreme high toxin production was found for each toxic species. Only about 10–15% of the hybrids showed higher resistance to the fungal species tested and lower contamination level of their toxins. The lacking correlations between resistance to different fungi and toxins suggest that resistance to different fungi and response to toxin contamination inherits independently, so a toxin analysis is necessary. For safety risk estimation, separated artificial and natural kernel infection and toxin data are needed against all pathogens. Higher resistance to A. flavus and F. verticillioides stabilizes or improves feed safety in hot and dry summers, balancing the harmful effect of climate changes. Resistance and toxin tests during variety registration is an utmost necessity. The exclusion of susceptible or highly susceptible hybrids from commercial production results in reduced toxin contamination. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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20 pages, 37698 KiB

Open AccessArticle

Synchronized Efficacy and Mechanism of Alkaline Fertilizer and Biocontrol Fungi for Fusariumoxysporum f. sp. cubense Tropical Race 4

by Yuanqiong Li, Shuting Jiang, Jiaquan Jiang, Chengxiang Gao, Xiuxiu Qi, Lidan Zhang, Shaolong Sun, Yinhai Dai and Xiaolin Fan

J. Fungi 2022, 8(3), 261; https://doi.org/10.3390/jof8030261 - 03 Mar 2022

Cited by 6 | Viewed by 2941

Abstract

The purpose of this study was to determine the effect and mechanism of alkaline fertilizer, bio-control fungi, and their synergistic application on control of Fusarium Tr4 incidence. Synchronized use of the alkaline fertilizer and biocontrol fungi eliminates rhizome browning and reduces the incidence [...] Read more.

The purpose of this study was to determine the effect and mechanism of alkaline fertilizer, bio-control fungi, and their synergistic application on control of Fusarium Tr4 incidence. Synchronized use of the alkaline fertilizer and biocontrol fungi eliminates rhizome browning and reduces the incidence rate of banana Fusarium wilt. The incidence of yellow leaves (ratio of yellow leaf to total leaf) and disease index in +Foc Tr4 CF treatment were the same (65%), while incidence of yellow leaves and disease index in +Foc Tr4 AFBCF were 31% and 33%, respectively. Under the stress of Foc Tr4 infection, the synergistic utilization of the alkaline fertilizer and biocontrol fungi would raise the activities of peroxidase, catalase and superoxide dismutase in banana roots. The root activity of banana was also increased. As a result, the banana height and stem diameter increments, shoot and root dry weight, accumulation of N, P and K in banana plants had been increased. The efficacy of the synergistic application of alkaline fertilizer and biocontrol fungi was not only reducing Foc Tr4 pathogen colonization and distribution in banana plants, but also preventing tylosis formation in vascular vessel effectively. Therefore, the normal transport of water and nutrients between underground and aboveground is ensured. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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21 pages, 4733 KiB

Open AccessArticle

Genetic Variability and Aggressiveness of Tilletia indica Isolates Causing Karnal Bunt in Wheat

by Aasma, Shahzad Asad, Muhammad Fayyaz, Khawar Majeed, Aziz ur Rehman, Sajid Ali, Jindong Liu, Awais Rasheed and Yamei Wang

J. Fungi 2022, 8(3), 219; https://doi.org/10.3390/jof8030219 - 23 Feb 2022

Cited by 2 | Viewed by 2062

Abstract

Karnal bunt caused by Tilletia indica is a quarantine disease of wheat causing huge economic losses due to the ban on the import of bunted grains. This study was designed to characterize pathogenicity, aggressiveness and genetic diversity of 68 Tilletia indica isolates collected [...] Read more.

Karnal bunt caused by Tilletia indica is a quarantine disease of wheat causing huge economic losses due to the ban on the import of bunted grains. This study was designed to characterize pathogenicity, aggressiveness and genetic diversity of 68 Tilletia indica isolates collected from different geographic regions of Pakistan. Forty-six isolates were tested for their pathogenicity on eight wheat varieties, out of which three were non-aggressive. The coefficient of infection (CI) ranged from 15.73% (PB-25) to 10% (PB-68, PB-60, and PB-43). The isolates collected from central Punjab showed higher infestation compared to other isolates. Among the wheat varieties used for the aggressiveness study, WL-711 showed susceptible reaction with 10.88% CI, while NIFA-Barsat, HD-29, Janbaz, Bakhtawar-92, Tatara, and AARI 2011 showed resistance to the highly resistant response. These isolates were amplified using 31 random amplified polymorphic DNA (RAPD) markers and 32 inter-simple sequence repeat (ISSR) markers for diversity analysis. The principal component analysis (PCA) and analysis of molecular variance (AMOVA) showed greater divergence among isolates collected from Punjab and Khyber Pakhtunkhwa (KPK), with a moderate level of admixture. The isolates from Faisalabad (Punjab) were more aggressive compared to isolates from KPK and were clearly separated based on PCA, indicating the significant genetic distance in the populations. Our findings will assist breeders and pathologists in better understanding the pathogenic variability in Tilletia indica and in subsequent disease management. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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17 pages, 5018 KiB

Open AccessArticle

Heat Shock Transcription Factor CgHSF1 Is Required for Melanin Biosynthesis, Appressorium Formation, and Pathogenicity in Colletotrichum gloeosporioides

by Xuesheng Gao, Qiannan Wang, Qingdeng Feng, Bei Zhang, Chaozu He, Hongli Luo and Bang An

J. Fungi 2022, 8(2), 175; https://doi.org/10.3390/jof8020175 - 11 Feb 2022

Cited by 15 | Viewed by 2751

Abstract

Heat shock transcription factors (HSFs) are a family of transcription regulators. Although HSFs’ functions in controlling the transcription of the molecular chaperone heat shock proteins and resistance to stresses are well established, their effects on the pathogenicity of plant pathogenic fungi remain unknown. [...] Read more.

Heat shock transcription factors (HSFs) are a family of transcription regulators. Although HSFs’ functions in controlling the transcription of the molecular chaperone heat shock proteins and resistance to stresses are well established, their effects on the pathogenicity of plant pathogenic fungi remain unknown. In this study, we analyze the role of CgHSF1 in the pathogenicity of Colletotrichum gloeosporioides and investigate the underlying mechanism. Failure to generate the Cghsf1 knock-out mutant suggested that the gene is essential for the viability of the fungus. Then, genetic depletion of the Cghsf1 was achieved by inserting the repressive promoter of nitrite reductase gene (PniiA) before its coding sequence. The mutant showed significantly decrease in the pathogenicity repression of appressorium formation, and severe defects in melanin biosynthesis. Moreover, four melanin synthetic genes were identified as direct targets of CgHSF1. Taken together, this work highlights the role of CgHSF1 in fungal pathogenicity via the transcriptional activation of melanin biosynthesis. Our study extends the understanding of fungal HSF1 proteins, especially their involvement in pathogenicity. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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14 pages, 1509 KiB

Open AccessArticle

Characterization of the Molecular Mechanisms of Resistance against DMI Fungicides in Cercospora beticola Populations from the Czech Republic

by Ram Kumar, Jana Mazakova, Asad Ali, Vishma Pratap Sur, Madhab Kumar Sen, Melvin D. Bolton, Marie Manasova, Pavel Rysanek and Miloslav Zouhar

J. Fungi 2021, 7(12), 1062; https://doi.org/10.3390/jof7121062 - 11 Dec 2021

Cited by 6 | Viewed by 3432

Abstract

Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most important foliar pathogen of sugar beet worldwide. Extensive reliance on fungicides to manage CLS has resulted in the evolution of fungicide resistance in C. beticola worldwide, including populations [...] Read more.

Cercospora leaf spot (CLS), caused by the fungal pathogen Cercospora beticola, is the most important foliar pathogen of sugar beet worldwide. Extensive reliance on fungicides to manage CLS has resulted in the evolution of fungicide resistance in C. beticola worldwide, including populations in the Czech Republic. One important class of fungicides used to manage CLS is the sterol demethylation inhibitors (DMI). The aim of our study was to assess DMI resistance in C. beticola from the Czech Republic and elucidate the molecular basis of DMI resistance in this population. A total of 50 isolates were collected in 2018 and 2019 from the major sugar beet growing regions of the Czech Republic and assessed for in vitro sensitivity to the DMI fungicides propiconazole, prochloraz, and epoxiconazole. These analyses identified three strains that exhibited 50% effective concentration (EC₅₀) values > 1.0 μg mL^–1 against respective fungicides, which were therefore considered resistant. In contrast, strains that exhibited lowest EC₅₀ values were considered sensitive. To explore the molecular basis of resistance in these three strains, the cytochrome P450-dependent sterol 14α-demethylase (Cyp51) gene was sequenced. Sequence analysis identified a Y464S mutation in all three resistant strains. To assess whether Cyp51 gene expression may play a role in DMI resistance, selected strains were grown in vitro with and without fungicide treatment. These analyses indicated that Cyp51 gene expression was significantly induced after fungicide treatment. Thus, we conclude that Y464S point mutation along with induced Cyp51 gene overexpression is likely responsible for resistance against DMI fungicides in C. beticola from the Czech Republic. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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22 pages, 4661 KiB

Open AccessReview

Suppression of Chitin-Triggered Immunity by Plant Fungal Pathogens: A Case Study of the Cucurbit Powdery Mildew Fungus Podosphaera xanthii

by Nisrine Bakhat, Alejandra Vielba-Fernández, Isabel Padilla-Roji, Jesús Martínez-Cruz, Álvaro Polonio, Dolores Fernández-Ortuño and Alejandro Pérez-García

J. Fungi 2023, 9(7), 771; https://doi.org/10.3390/jof9070771 - 21 Jul 2023

Cited by 5 | Viewed by 2326

Abstract

Fungal pathogens are significant plant-destroying microorganisms that present an increasing threat to the world’s crop production. Chitin is a crucial component of fungal cell walls and a conserved MAMP (microbe-associated molecular pattern) that can be recognized by specific plant receptors, activating chitin-triggered immunity. [...] Read more.

Fungal pathogens are significant plant-destroying microorganisms that present an increasing threat to the world’s crop production. Chitin is a crucial component of fungal cell walls and a conserved MAMP (microbe-associated molecular pattern) that can be recognized by specific plant receptors, activating chitin-triggered immunity. The molecular mechanisms underlying the perception of chitin by specific receptors are well known in plants such as rice and Arabidopsis thaliana and are believed to function similarly in many other plants. To become a plant pathogen, fungi have to suppress the activation of chitin-triggered immunity. Therefore, fungal pathogens have evolved various strategies, such as prevention of chitin digestion or interference with plant chitin receptors or chitin signaling, which involve the secretion of fungal proteins in most cases. Since chitin immunity is a very effective defensive response, these fungal mechanisms are believed to work in close coordination. In this review, we first provide an overview of the current understanding of chitin-triggered immune signaling and the fungal proteins developed for its suppression. Second, as an example, we discuss the mechanisms operating in fungal biotrophs such as powdery mildew fungi, particularly in the model species Podosphaera xanthii, the main causal agent of powdery mildew in cucurbits. The key role of fungal effector proteins involved in the modification, degradation, or sequestration of immunogenic chitin oligomers is discussed in the context of fungal pathogenesis and the promotion of powdery mildew disease. Finally, the use of this fundamental knowledge for the development of intervention strategies against powdery mildew fungi is also discussed. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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23 pages, 1790 KiB

Open AccessReview

Fungal Secondary Metabolites and Small RNAs Enhance Pathogenicity during Plant-Fungal Pathogen Interactions

by Johannes Mapuranga, Jiaying Chang, Lirong Zhang, Na Zhang and Wenxiang Yang

J. Fungi 2023, 9(1), 4; https://doi.org/10.3390/jof9010004 - 20 Dec 2022

Cited by 4 | Viewed by 3039

Abstract

Fungal plant pathogens use proteinaceous effectors as well as newly identified secondary metabolites (SMs) and small non-coding RNA (sRNA) effectors to manipulate the host plant’s defense system via diverse plant cell compartments, distinct organelles, and many host genes. However, most molecular studies of [...] Read more.

Fungal plant pathogens use proteinaceous effectors as well as newly identified secondary metabolites (SMs) and small non-coding RNA (sRNA) effectors to manipulate the host plant’s defense system via diverse plant cell compartments, distinct organelles, and many host genes. However, most molecular studies of plant–fungal interactions have focused on secreted effector proteins without exploring the possibly equivalent functions performed by fungal (SMs) and sRNAs, which are collectively known as “non-proteinaceous effectors”. Fungal SMs have been shown to be generated throughout the plant colonization process, particularly in the early biotrophic stages of infection. The fungal repertoire of non-proteinaceous effectors has been broadened by the discovery of fungal sRNAs that specifically target plant genes involved in resistance and defense responses. Many RNAs, particularly sRNAs involved in gene silencing, have been shown to transmit bidirectionally between fungal pathogens and their hosts. However, there are no clear functional approaches to study the role of these SM and sRNA effectors. Undoubtedly, fungal SM and sRNA effectors are now a treasured land to seek. Therefore, understanding the role of fungal SM and sRNA effectors may provide insights into the infection process and identification of the interacting host genes that are targeted by these effectors. This review discusses the role of fungal SMs and sRNAs during plant-fungal interactions. It will also focus on the translocation of sRNA effectors across kingdoms, the application of cross-kingdom RNA interference in managing plant diseases and the tools that can be used to predict and study these non-proteinaceous effectors. Full article

(This article belongs to the Special Issue Plant Fungal Pathogenesis 2022)

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