Editorial

3 pages, 194 KiB

Open AccessEditorial

by Kamel A. Abd-Elsalam

J. Fungi 2021, 7(8), 583; https://doi.org/10.3390/jof7080583 - 21 Jul 2021

Cited by 15 | Viewed by 2813

Fungal nanotechnology (FN) or myconanotechnology is a novel word which was originally introduced in 2009 by Rai M [...] Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

Research

Jump to: Editorial, Review

15 pages, 3550 KiB

Open AccessArticle

Visible-Light-Driven Ag-Modified TiO₂ Thin Films Anchored on Bamboo Material with Antifungal Memory Activity against Aspergillus niger

by Jingpeng Li, Rumin Ma, Zaixing Wu, Sheng He, Yuhe Chen, Ruihua Bai and Jin Wang

J. Fungi 2021, 7(8), 592; https://doi.org/10.3390/jof7080592 - 23 Jul 2021

Cited by 12 | Viewed by 1977

Abstract

A round-the-clock photocatalyst with energy-storage ability has piqued the interest of researchers for removing microbial contaminants from indoor environments. This work presents a moderate round-the-clock method for inhibiting the growth of fungus spores on bamboo materials using Ag-modified TiO₂ thin films. Photoactivated [...] Read more.

A round-the-clock photocatalyst with energy-storage ability has piqued the interest of researchers for removing microbial contaminants from indoor environments. This work presents a moderate round-the-clock method for inhibiting the growth of fungus spores on bamboo materials using Ag-modified TiO₂ thin films. Photoactivated antifungal coating with catalytic memory activity was assembled on a hydrophilic bamboo by first anchoring anatase TiO₂ thin films (TB) via hydrogen bonding and then decorating them with Ag nanoparticles (ATB) via electrostatic interactions. Antifungal test results show that the Ag/TiO₂ composite films grown on the bamboo surface produced a synergistic antifungal mechanism under both light and dark conditions. Interestingly, post-illumination catalytic memory was observed for ATB, as demonstrated by the inhibition of Aspergillus niger (A. niger) spores, in the dark after visible light was removed, which could be attributed to the transfer of photoexcited electrons from TiO₂ to Ag, their trapping on Ag under visible-light illumination, and their release in the dark after visible light was removed. The mechanism study revealed that the immobilized Ag nanoparticles served the role of “killing two birds with one stone”: increasing visible-light absorption through surface plasmon resonance, preventing photogenerated electron–hole recombination by trapping electrons, and contributing to the generation of ●O₂⁻and ●OH. This discovery creates a pathway for the continuous removal of indoor air pollutants such as volatile organic compounds, bacteria, and fungus in the day and night time. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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25 pages, 8780 KiB

Open AccessArticle

Rhizopus oryzae-Mediated Green Synthesis of Magnesium Oxide Nanoparticles (MgO-NPs): A Promising Tool for Antimicrobial, Mosquitocidal Action, and Tanning Effluent Treatment

by Saad El-Din Hassan, Amr Fouda, Ebrahim Saied, Mohamed M. S. Farag, Ahmed M. Eid, Mohammed G. Barghoth, Mohamed A. Awad, Mohammed F. Hamza and Mohamed F. Awad

J. Fungi 2021, 7(5), 372; https://doi.org/10.3390/jof7050372 - 10 May 2021

Cited by 102 | Viewed by 5982

Abstract

The metabolites of the fungal strain Rhizopus oryaze were used as a biocatalyst for the green-synthesis of magnesium oxide nanoparticles (MgO-NPs). The production methodology was optimized to attain the maximum productivity as follows: 4 mM of precursor, at pH 8, incubation temperature of [...] Read more.

The metabolites of the fungal strain Rhizopus oryaze were used as a biocatalyst for the green-synthesis of magnesium oxide nanoparticles (MgO-NPs). The production methodology was optimized to attain the maximum productivity as follows: 4 mM of precursor, at pH 8, incubation temperature of 35 °C, and reaction time of 36 h between metabolites and precursor. The as-formed MgO-NPs were characterized by UV-Vis spectroscopy, TEM, SEM-EDX, XRD, DLS, FT-IR, and XPS analyses. These analytical techniques proved to gain crystalline, homogenous, and well-dispersed spherical MgO-NPs with an average size of 20.38 ± 9.9 nm. The potentiality of MgO-NPs was dose- and time-dependent. The biogenic MgO-NPs was found to be a promising antimicrobial agent against the pathogens including Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans with inhibition zones of 10.6 ± 0.4, 11.5 ± 0.5, 13.7 ± 0.5, 14.3 ± 0.7, and 14.7 ± 0.6 mm, respectively, at 200 μg mL^–1. Moreover, MgO-NPs manifested larvicidal and adult repellence activity against Culex pipiens at very low concentrations. The highest decolorization percentages of tanning effluents were 95.6 ± 1.6% at 100 µg/ 100 mL after 180 min. At this condition, the physicochemical parameters of tannery effluents, including TSS, TDS, BOD, COD, and conductivity were reduced with percentages of 97.9%, 98.2%, 87.8%, 95.9%, and 97.3%, respectively. Moreover, the chromium ion was adsorbed with percentages of 98.2% at optimum experimental conditions. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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16 pages, 39715 KiB

Open AccessArticle

Differential Antimycotic and Antioxidant Potentials of Chemically Synthesized Zinc-Based Nanoparticles Derived from Different Reducing/Complexing Agents against Pathogenic Fungi of Maize Crop

by Anu Kalia, Jashanpreet Kaur, Manisha Tondey, Pooja Manchanda, Pulkit Bindra, Mousa A. Alghuthaymi, Ashwag Shami and Kamel A. Abd-Elsalam

J. Fungi 2021, 7(3), 223; https://doi.org/10.3390/jof7030223 - 18 Mar 2021

Cited by 8 | Viewed by 2583

Abstract

The present study aimed for the synthesis, characterization, and comparative evaluation of anti-oxidant and anti-fungal potentials of zinc-based nanoparticles (ZnNPs) by using different reducing or organic complexing-capping agents. The synthesized ZnNPs exhibited quasi-spherical to hexagonal shapes with average particle sizes ranging from 8 [...] Read more.

The present study aimed for the synthesis, characterization, and comparative evaluation of anti-oxidant and anti-fungal potentials of zinc-based nanoparticles (ZnNPs) by using different reducing or organic complexing-capping agents. The synthesized ZnNPs exhibited quasi-spherical to hexagonal shapes with average particle sizes ranging from 8 to 210 nm. The UV-Vis spectroscopy of the prepared ZnNPs showed variation in the appearance of characteristic absorption peak(s) for the various reducing/complexing agents i.e., 210 (NaOH and NaBH₄), 220 (albumin, and thiourea), 260 and 330 (starch), and 351 nm (cellulose) for wavelengths spanning over 190–800 nm. The FT-IR spectroscopy of the synthesized ZnNPs depicted the functional chemical group diversity. On comparing the antioxidant potential of these ZnNPs, NaOH as reducing agent, (NaOH (RA)) derived ZnNPs presented significantly higher DPPH radical scavenging potential compared to other ZnNPs. The anti-mycotic potential of the ZnNPs as performed through an agar well diffusion assay exhibited variability in the extent of inhibition of the fungal mycelia with maximum inhibition at the highest concentration (40 mg L⁻¹). The NaOH (RA)-derived ZnNPs showcased maximum mycelial inhibition compared to other ZnNPs. Further, incubation of the total genomic DNA with the most effective NaOH (RA)-derived ZnNPs led to intercalation or disintegration of the DNA of all the three fungal pathogens of maize with maximum DNA degrading effect on Macrophomina phaseolina genomic DNA. This study thus identified that differences in size and surface functionalization with the protein (albumin)/polysaccharides (starch, cellulose) diminishes the anti-oxidant and anti-mycotic potential of the generated ZnNPs. However, the NaOH emerged as the best reducing agent for the generation of uniform nano-scale ZnNPs which possessed comparably greater anti-oxidant and antimycotic activities against the three test maize pathogenic fungal cultures. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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18 pages, 4585 KiB

Open AccessArticle

Bacillus megaterium-Mediated Synthesis of Selenium Nanoparticles and Their Antifungal Activity against Rhizoctonia solani in Faba Bean Plants

by Amr H. Hashem, Amer M. Abdelaziz, Ahmed A. Askar, Hossam M. Fouda, Ahmed M. A. Khalil, Kamel A. Abd-Elsalam and Mona M. Khaleil

J. Fungi 2021, 7(3), 195; https://doi.org/10.3390/jof7030195 - 09 Mar 2021

Cited by 100 | Viewed by 5116

Abstract

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as [...] Read more.

Rhizoctonia root-rot disease causes severe economic losses in a wide range of crops, including Vicia faba worldwide. Currently, biosynthesized nanoparticles have become super-growth promoters as well as antifungal agents. In this study, biosynthesized selenium nanoparticles (Se-NPs) have been examined as growth promoters as well as antifungal agents against Rhizoctonia solani RCMB 031001 in vitro and in vivo. Se-NPs were synthesized biologically by Bacillus megaterium ATCC 55000 and characterized by using UV-Vis spectroscopy, XRD, dynamic light scattering (DLS), and transmission electron microscopy (TEM) imaging. TEM and DLS images showed that Se-NPs are mono-dispersed spheres with a mean diameter of 41.2 nm. Se-NPs improved healthy Vicia faba cv. Giza 716 seed germination, morphological, metabolic indicators, and yield. Furthermore, Se-NPs exhibited influential antifungal activity against R. solani in vitro as well as in vivo. Results revealed that minimum inhibition and minimum fungicidal concentrations of Se-NPs were 0.0625 and 1 mM, respectively. Moreover, Se-NPs were able to decrease the pre-and post-emergence of R. solani damping-off and minimize the severity of root rot disease. The most effective treatment method is found when soaking and spraying were used with each other followed by spraying and then soaking individually. Likewise, Se-NPs improve morphological and metabolic indicators and yield significantly compared with infected control. In conclusion, biosynthesized Se-NPs by B. megaterium ATCC 55000 are a promising and effective agent against R. solani damping-off and root rot diseases in Vicia faba as well as plant growth inducer. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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23 pages, 15367 KiB

Open AccessArticle

Development of Nano-Antifungal Therapy for Systemic and Endemic Mycoses

by Jorge H. Martínez-Montelongo, Iliana E. Medina-Ramírez, Yolanda Romo-Lozano, Antonio González-Gutiérrez and Jorge E. Macías-Díaz

J. Fungi 2021, 7(2), 158; https://doi.org/10.3390/jof7020158 - 23 Feb 2021

Cited by 8 | Viewed by 2471

Abstract

Fungal mycoses have become an important health and environmental concern due to the numerous deleterious side effects on the well-being of plants and humans. Antifungal therapy is limited, expensive, and unspecific (causes toxic effects), thus, more efficient alternatives need to be developed. In [...] Read more.

Fungal mycoses have become an important health and environmental concern due to the numerous deleterious side effects on the well-being of plants and humans. Antifungal therapy is limited, expensive, and unspecific (causes toxic effects), thus, more efficient alternatives need to be developed. In this work, Copper (I) Iodide (CuI) nanomaterials (NMs) were synthesized and fully characterized, aiming to develop efficient antifungal agents. The bioactivity of CuI NMs was evaluated using Sporothrix schenckii and Candida albicans as model organisms. CuI NMs were prepared as powders and as colloidal suspensions by a two-step reaction: first, the CuI

_{2}

controlled precipitation, followed by hydrazine reduction. Biopolymers (Arabic gum and chitosan) were used as surfactants to control the size of the CuI materials and to enhance its antifungal activity. The materials (powders and colloids) were characterized by SEM-EDX and AFM. The materials exhibit a hierarchical 3D shell morphology composed of ordered nanostructures. Excellent antifungal activity is shown by the NMs against pathogenic fungal strains, due to the simultaneous and multiple mechanisms of the composites to combat fungi. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of CuI-AG and CuI-Chitosan are below 50 μg/mL (with 5 h of exposition). Optical and Atomic Force Microscopy (AFM) analyses demonstrate the capability of the materials to disrupt biofilm formation. AFM also demonstrates the ability of the materials to adhere and penetrate fungal cells, followed by their lysis and death. Following the concept of safe by design, the biocompatibility of the materials was tested. The hemolytic activity of the materials was evaluated using red blood cells. Our results indicate that the materials show an excellent antifungal activity at lower doses of hemolytic disruption. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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13 pages, 4006 KiB

Open AccessArticle

Biosynthesis of Silver Nanoparticles Using Onion Endophytic Bacterium and Its Antifungal Activity against Rice Pathogen Magnaporthe oryzae

by Ezzeldin Ibrahim, Jinyan Luo, Temoor Ahmed, Wenge Wu, Chenqi Yan and Bin Li

J. Fungi 2020, 6(4), 294; https://doi.org/10.3390/jof6040294 - 18 Nov 2020

Cited by 32 | Viewed by 3294

Abstract

Biosynthesis of silver nanoparticles (AgNPs) using endophytic bacteria is a safe alternative to the traditional chemical method. The purpose of this research is to biosynthesize AgNPs using endophytic bacterium Bacillus endophyticus strain H3 isolated from onion. The biosynthesized AgNPs with sizes from 4.17 [...] Read more.

Biosynthesis of silver nanoparticles (AgNPs) using endophytic bacteria is a safe alternative to the traditional chemical method. The purpose of this research is to biosynthesize AgNPs using endophytic bacterium Bacillus endophyticus strain H3 isolated from onion. The biosynthesized AgNPs with sizes from 4.17 to 26.9 nm were confirmed and characterized by various physicochemical techniques such as Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV-visible spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) in addition to an energy dispersive spectrum (EDS) profile. The biosynthesized AgNPs at a concentration of 40 μg/mL had a strong antifungal activity against rice blast pathogen Magnaporthe oryzae with an inhibition rate of 88% in mycelial diameter. Moreover, the biosynthesized AgNPs significantly inhibited spore germination and appressorium formation of M. oryzae. Additionally, microscopic observation showed that mycelia morphology was swollen and abnormal when dealing with AgNPs. Overall, the current study revealed that AgNPs could protect rice plants against fungal infections. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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10 pages, 1847 KiB

Open AccessArticle

Fungus Aspergillus niger Processes Exogenous Zinc Nanoparticles into a Biogenic Oxalate Mineral

by Martin Šebesta, Martin Urík, Marek Bujdoš, Marek Kolenčík, Ivo Vávra, Edmund Dobročka, Hyunjung Kim and Peter Matúš

J. Fungi 2020, 6(4), 210; https://doi.org/10.3390/jof6040210 - 08 Oct 2020

Cited by 7 | Viewed by 2462

Abstract

Zinc oxide nanoparticles (ZnO NPs) belong to the most widely used nanoparticles in both commercial products and industrial applications. Hence, they are frequently released into the environment. Soil fungi can affect the mobilization of zinc from ZnO NPs in soils, and thus they [...] Read more.

Zinc oxide nanoparticles (ZnO NPs) belong to the most widely used nanoparticles in both commercial products and industrial applications. Hence, they are frequently released into the environment. Soil fungi can affect the mobilization of zinc from ZnO NPs in soils, and thus they can heavily influence the mobility and bioavailability of zinc there. Therefore, ubiquitous soil fungus Aspergillus niger was selected as a test organism to evaluate the fungal interaction with ZnO NPs. As anticipated, the A. niger strain significantly affected the stability of particulate forms of ZnO due to the acidification of its environment. The influence of ZnO NPs on fungus was compared to the aqueous Zn cations and to bulk ZnO as well. Bulk ZnO had the least effect on fungal growth, while the response of A. niger to ZnO NPs was comparable with ionic zinc. Our results have shown that soil fungus can efficiently bioaccumulate Zn that was bioextracted from ZnO. Furthermore, it influences Zn bioavailability to plants by ZnO NPs transformation to stable biogenic minerals. Hence, a newly formed biogenic mineral phase of zinc oxalate was identified after the experiment with A. niger strain’s extracellular metabolites highlighting the fungal significance in zinc biogeochemistry. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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19 pages, 8195 KiB

Open AccessArticle

Mycosynthesis of ZnO Nanoparticles Using Trichoderma spp. Isolated from Rhizosphere Soils and Its Synergistic Antibacterial Effect against Xanthomonas oryzae pv. oryzae

by Balagangadharaswamy Shobha, Thimappa Ramachandrappa Lakshmeesha, Mohammad Azam Ansari, Ahmad Almatroudi, Mohammad A. Alzohairy, Sumanth Basavaraju, Ramesha Alurappa, Siddapura Ramachandrappa Niranjana and Srinivas Chowdappa

J. Fungi 2020, 6(3), 181; https://doi.org/10.3390/jof6030181 - 20 Sep 2020

Cited by 44 | Viewed by 5299

Abstract

The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation of Trichoderma spp. (T. harzianum (PGT4), T. reesei (PGT5) [...] Read more.

The Plant Growth Promoting Fungi (PGPF) is used as a source of biofertilizers due to their production of secondary metabolites and beneficial effects on plants. The present work is focused on the co-cultivation of Trichoderma spp. (T. harzianum (PGT4), T. reesei (PGT5) and T. reesei (PGT13)) and the production of secondary metabolites from mono and co-culture and mycosynthesis of zinc oxide nanoparticles (ZnO NPs), which were characterized by a UV visible spectrophotometer, Powder X-ray Diffraction (PXRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) with Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscope (TEM) and Selected Area (Electron) Diffraction (SAED) patterns. The fungal secondary metabolite crude was extracted from the mono and co-culture of Trichoderma spp. And were analyzed by GC-MS, which was further subjected for antibacterial activity against Xanthomonas oryzae pv. Oryzae, the causative organism for Bacterial Leaf Blight (BLB) in rice. Our results showed that the maximum zone of inhibition was recorded from the co-culture of Trichoderma spp. rather than mono cultures, which indicates that co-cultivation of beneficial fungi can stimulate the synthesis of novel secondary metabolites better than in monocultures. ZnO NPs were synthesized from fungal secondary metabolites of mono cultures of Trichoderma harzianum (PGT4), Trichoderma reesei (PGT5), Trichoderma reesei (PGT13) and co-culture (PGT4 + PGT5 + PGT13). These ZnO NPs were checked for antibacterial activity against Xoo, which was found to be of a dose-dependent manner. In summary, the biosynthesized ZnO NPs and secondary metabolites from co-culture of Trichoderma spp. are ecofriendly and can be used as an alternative for chemical fertilizers in agriculture. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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20 pages, 4807 KiB

Open AccessArticle

Copper-Chitosan Nanocomposite Hydrogels Against Aflatoxigenic Aspergillus flavus from Dairy Cattle Feed

by Kamel A. Abd-Elsalam, Mousa A. Alghuthaymi, Ashwag Shami, Margarita S. Rubina, Sergey S. Abramchuk, Eleonora V. Shtykova and Alexander Yu. Vasil’kov

J. Fungi 2020, 6(3), 112; https://doi.org/10.3390/jof6030112 - 21 Jul 2020

Cited by 22 | Viewed by 3835

Abstract

The integration of copper nanoparticles as antifungal agents in polymeric matrices to produce copper polymer nanocomposites has shown excellent results in preventing the growth of a wide variety of toxigenic fungi. Copper-chitosan nanocomposite-based chitosan hydrogels (Cu-Chit/NCs hydrogel) were prepared using a metal vapor [...] Read more.

The integration of copper nanoparticles as antifungal agents in polymeric matrices to produce copper polymer nanocomposites has shown excellent results in preventing the growth of a wide variety of toxigenic fungi. Copper-chitosan nanocomposite-based chitosan hydrogels (Cu-Chit/NCs hydrogel) were prepared using a metal vapor synthesis (MVS) and the resulting samples were described by transmission electron microscopy (TEM), X-ray fluorescence analysis (XRF), and small-angle X-ray scattering (SAXS). Aflatoxin-producing medium and VICAM aflatoxins tests were applied to evaluate their ability to produce aflatoxins through various strains of Aspergillus flavus associated with peanut meal and cotton seeds. Aflatoxin production capacity in four fungal media outlets revealed that 13 tested isolates were capable of producing both aflatoxin B1 and B2. Only 2 A. flavus isolates (Af11 and Af 20) fluoresced under UV light in the A. flavus and parasiticus Agar (AFPA) medium. PCR was completed using two specific primers targeting aflP and aflA genes involved in the synthetic track of aflatoxin. Nevertheless, the existence of aflP and aflA genes indicated some correlation with the development of aflatoxin. A unique DNA fragment of the expected 236 bp and 412 bp bands for aflP and aflA genes in A. flavus isolates, although non-PCR fragments have been observed in many other Aspergillus species. This study shows the antifungal activity of Cu-Chit/NCs hydrogels against aflatoxigenic strains of A. flavus. Our results reveal that the antifungal activity of nanocomposites in vitro can be effective depending on the type of fungal strain and nanocomposite concentration. SDS-PAGE and native proteins explain the apparent response of cellular proteins in the presence of Cu-Chit/NCs hydrogels. A. flavus treated with a high concentration of Cu-Chit/NCs hydrogels that can decrease or produce certain types of proteins. Cu-Chit/NCs hydrogel decreases the effect of G6DP isozyme while not affecting the activity of peroxidase isozymes in tested isolates. Additionally, microscopic measurements of scanning electron microscopy (SEM) showed damage to the fungal cell membranes. Cu-Chit/NC_S hydrogel is an innovative nano-biopesticide produced by MVS is employed in food and feed to induce plant defense against toxigenic fungi. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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Review

Jump to: Editorial, Research

19 pages, 3003 KiB

Open AccessReview

Antifungal Nano-Therapy in Veterinary Medicine: Current Status and Future Prospects

by Mousa A. Alghuthaymi, Atef A. Hassan, Anu Kalia, Rasha M. H. Sayed El Ahl, Ahmed A. M. El Hamaky, Patrik Oleksak, Kamil Kuca and Kamel A. Abd-Elsalam

J. Fungi 2021, 7(7), 494; https://doi.org/10.3390/jof7070494 - 22 Jun 2021

Cited by 14 | Viewed by 3385

Abstract

The global recognition for the potential of nanoproducts and processes in human biomedicine has given impetus for the development of novel strategies for rapid, reliable, and proficient diagnosis, prevention, and control of animal diseases. Nanomaterials exhibit significant antifungal and antimycotoxin activities against mycosis [...] Read more.

The global recognition for the potential of nanoproducts and processes in human biomedicine has given impetus for the development of novel strategies for rapid, reliable, and proficient diagnosis, prevention, and control of animal diseases. Nanomaterials exhibit significant antifungal and antimycotoxin activities against mycosis and mycotoxicosis disorders in animals, as evidenced through reports published over the recent decade and more. These nanoantifungals can be potentially utilized for the development of a variety of products of pharmaceutical and biomedical significance including the nano-scale vaccines, adjuvants, anticancer and gene therapy systems, farm disinfectants, animal husbandry, and nutritional products. This review will provide details on the therapeutic and preventative aspects of nanoantifungals against diverse fungal and mycotoxin-related diseases in animals. The predominant mechanisms of action of these nanoantifungals and their potential as antifungal and cytotoxicity-causing agents will also be illustrated. Also, the other theragnostic applications of nanoantifungals in veterinary medicine will be identified. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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42 pages, 6127 KiB

Open AccessReview

Lichens—A Potential Source for Nanoparticles Fabrication: A Review on Nanoparticles Biosynthesis and Their Prospective Applications

by Reham Samir Hamida, Mohamed Abdelaal Ali, Nabila Elsayed Abdelmeguid, Mayasar Ibrahim Al-Zaban, Lina Baz and Mashael Mohammed Bin-Meferij

J. Fungi 2021, 7(4), 291; https://doi.org/10.3390/jof7040291 - 12 Apr 2021

Cited by 38 | Viewed by 5781

Abstract

Green synthesis of nanoparticles (NPs) is a safe, eco-friendly, and relatively inexpensive alternative to conventional routes of NPs production. These methods require natural resources such as cyanobacteria, algae, plants, fungi, lichens, and naturally extracted biomolecules such as pigments, vitamins, polysaccharides, proteins, and enzymes [...] Read more.

Green synthesis of nanoparticles (NPs) is a safe, eco-friendly, and relatively inexpensive alternative to conventional routes of NPs production. These methods require natural resources such as cyanobacteria, algae, plants, fungi, lichens, and naturally extracted biomolecules such as pigments, vitamins, polysaccharides, proteins, and enzymes to reduce bulk materials (the target metal salts) into a nanoscale product. Synthesis of nanomaterials (NMs) using lichen extracts is a promising eco-friendly, simple, low-cost biological synthesis process. Lichens are groups of organisms including multiple types of fungi and algae that live in symbiosis. Until now, the fabrication of NPs using lichens has remained largely unexplored, although the role of lichens as natural factories for synthesizing NPs has been reported. Lichens have a potential reducible activity to fabricate different types of NMs, including metal and metal oxide NPs and bimetallic alloys and nanocomposites. These NPs exhibit promising catalytic and antidiabetic, antioxidant, and antimicrobial activities. To the best of our knowledge, this review provides, for the first time, an overview of the main published studies concerning the use of lichen for nanofabrication and the applications of these NMs in different sectors. Moreover, the possible mechanisms of biosynthesis are discussed, together with the various optimization factors influencing the biological synthesis and toxicity of NPs. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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30 pages, 1629 KiB

Open AccessReview

Fungal–Metal Interactions: A Review of Toxicity and Homeostasis

by Janelle R. Robinson, Omoanghe S. Isikhuemhen and Felicia N. Anike

J. Fungi 2021, 7(3), 225; https://doi.org/10.3390/jof7030225 - 18 Mar 2021

Cited by 64 | Viewed by 6952

Abstract

Metal nanoparticles used as antifungals have increased the occurrence of fungal–metal interactions. However, there is a lack of knowledge about how these interactions cause genomic and physiological changes, which can produce fungal superbugs. Despite interest in these interactions, there is limited understanding of [...] Read more.

Metal nanoparticles used as antifungals have increased the occurrence of fungal–metal interactions. However, there is a lack of knowledge about how these interactions cause genomic and physiological changes, which can produce fungal superbugs. Despite interest in these interactions, there is limited understanding of resistance mechanisms in most fungi studied until now. We highlight the current knowledge of fungal homeostasis of zinc, copper, iron, manganese, and silver to comprehensively examine associated mechanisms of resistance. Such mechanisms have been widely studied in Saccharomyces cerevisiae, but limited reports exist in filamentous fungi, though they are frequently the subject of nanoparticle biosynthesis and targets of antifungal metals. In most cases, microarray analyses uncovered resistance mechanisms as a response to metal exposure. In yeast, metal resistance is mainly due to the down-regulation of metal ion importers, utilization of metallothionein and metallothionein-like structures, and ion sequestration to the vacuole. In contrast, metal resistance in filamentous fungi heavily relies upon cellular ion export. However, there are instances of resistance that utilized vacuole sequestration, ion metallothionein, and chelator binding, deleting a metal ion importer, and ion storage in hyphal cell walls. In general, resistance to zinc, copper, iron, and manganese is extensively reported in yeast and partially known in filamentous fungi; and silver resistance lacks comprehensive understanding in both. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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22 pages, 3994 KiB

Open AccessReview

Fusarium as a Novel Fungus for the Synthesis of Nanoparticles: Mechanism and Applications

by Mahendra Rai, Shital Bonde, Patrycja Golinska, Joanna Trzcińska-Wencel, Aniket Gade, Kamel A. Abd-Elsalam, Sudhir Shende, Swapnil Gaikwad and Avinash P. Ingle

J. Fungi 2021, 7(2), 139; https://doi.org/10.3390/jof7020139 - 15 Feb 2021

Cited by 90 | Viewed by 6853

Abstract

Nanotechnology is a new and developing branch that has revolutionized the world by its applications in various fields including medicine and agriculture. In nanotechnology, nanoparticles play an important role in diagnostics, drug delivery, and therapy. The synthesis of nanoparticles by fungi is a [...] Read more.

Nanotechnology is a new and developing branch that has revolutionized the world by its applications in various fields including medicine and agriculture. In nanotechnology, nanoparticles play an important role in diagnostics, drug delivery, and therapy. The synthesis of nanoparticles by fungi is a novel, cost-effective and eco-friendly approach. Among fungi, Fusarium spp. play an important role in the synthesis of nanoparticles and can be considered as a nanofactory for the fabrication of nanoparticles. The synthesis of silver nanoparticles (AgNPs) from Fusarium, its mechanism and applications are discussed in this review. The synthesis of nanoparticles from Fusarium is the biogenic and green approach. Fusaria are found to be a versatile biological system with the ability to synthesize nanoparticles extracellularly. Different species of Fusaria have the potential to synthesise nanoparticles. Among these, F. oxysporum has demonstrated a high potential for the synthesis of AgNPs. It is hypothesised that NADH-dependent nitrate reductase enzyme secreted by F. oxysporum is responsible for the reduction of aqueous silver ions into AgNPs. The toxicity of nanoparticles depends upon the shape, size, surface charge, and the concentration used. The nanoparticles synthesised by different species of Fusaria can be used in medicine and agriculture. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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20 pages, 2939 KiB

Open AccessReview

Nanohybrid Antifungals for Control of Plant Diseases: Current Status and Future Perspectives

by Mousa A. Alghuthaymi, Rajkuberan C., Rajiv P., Anu Kalia, Kanchan Bhardwaj, Prerna Bhardwaj, Kamel A. Abd-Elsalam, Martin Valis and Kamil Kuca

J. Fungi 2021, 7(1), 48; https://doi.org/10.3390/jof7010048 - 13 Jan 2021

Cited by 43 | Viewed by 5355

Abstract

The changing climatic conditions have led to the concurrent emergence of virulent microbial pathogens that attack crop plants and exhibit yield and quality deterring impacts on the affected crop. To counteract, the widespread infections of fungal pathogens and post-harvest diseases it is highly [...] Read more.

The changing climatic conditions have led to the concurrent emergence of virulent microbial pathogens that attack crop plants and exhibit yield and quality deterring impacts on the affected crop. To counteract, the widespread infections of fungal pathogens and post-harvest diseases it is highly warranted to develop sustainable techniques and tools bypassing traditional agriculture practices. Nanotechnology offers a solution to the problems in disease management in a simple lucid way. These technologies are revolutionizing the scientific/industrial sectors. Likewise, in agriculture, the nano-based tools are of great promise particularly for the development of potent formulations ensuring proper delivery of agrochemicals, nutrients, pesticides/insecticides, and even growth regulators for enhanced use efficiency. The development of novel nanocomposites for improved management of fungal diseases can mitigate the emergence of resilient and persistent fungal pathogens and the loss of crop produce due to diseases they cause. Therefore, in this review, we collectively manifest the role of nanocomposites for the management of fungal diseases. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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21 pages, 3972 KiB

Open AccessReview

Pleurotus Macrofungi-Assisted Nanoparticle Synthesis and Its Potential Applications: A Review

by Kanchan Bhardwaj, Anirudh Sharma, Neeraj Tejwan, Sonali Bhardwaj, Prerna Bhardwaj, Eugenie Nepovimova, Ashwag Shami, Anu Kalia, Anil Kumar, Kamel A. Abd-Elsalam and Kamil Kuča

J. Fungi 2020, 6(4), 351; https://doi.org/10.3390/jof6040351 - 09 Dec 2020

Cited by 40 | Viewed by 5036

Abstract

Research and innovation in nanoparticles (NPs) synthesis derived from biomaterials have gained much attention due to their unique characteristics, such as low-cost, easy synthesis methods, high water solubility, and eco-friendly nature. NPs derived from macrofungi, including various mushroom species, such as Agaricus bisporus, [...] Read more.

Research and innovation in nanoparticles (NPs) synthesis derived from biomaterials have gained much attention due to their unique characteristics, such as low-cost, easy synthesis methods, high water solubility, and eco-friendly nature. NPs derived from macrofungi, including various mushroom species, such as Agaricus bisporus, Pleurotus spp., Lentinus spp., and Ganoderma spp. are well known to possess high nutritional, immune-modulatory, antimicrobial (antibacterial, antifungal and antiviral), antioxidant, and anticancerous properties. Fungi have intracellular metal uptake ability and maximum wall binding capacity; because of which, they have high metal tolerance and bioaccumulation ability. Primarily, two methods have been comprehended in the literature to synthesize metal NPs from macrofungi, i.e., the intracellular method, which refers to NP synthesis inside fungal cells by transportation of ions in the presence of enzymes; and the extracellular method, which refers to the treatment of fungal biomolecules aqueous filtrate with a metal precursor. Pleurotus derived metal NPs are known to inhibit the growth of numerous foodborne pathogenic bacteria and fungi. To the best of our knowledge, there is no such review article reported in the literature describing the synthesis and complete application and mechanism of NPs derived from macrofungi. Herein, we intend to summarize the progressive research on macrofungi derived NPs regarding their synthesis as well as applications in the area of antimicrobial (antibacterial & antifungal), anticancer, antioxidant, catalytic and food preservation. Additionally, the challenges associated with NPs synthesis will also be discussed. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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29 pages, 2294 KiB

Open AccessReview

Zinc-Based Nanomaterials for Diagnosis and Management of Plant Diseases: Ecological Safety and Future Prospects

by Anu Kalia, Kamel A. Abd-Elsalam and Kamil Kuca

J. Fungi 2020, 6(4), 222; https://doi.org/10.3390/jof6040222 - 13 Oct 2020

Cited by 52 | Viewed by 6156

Abstract

A facet of nanorenaissance in plant pathology hailed the research on the development and application of nanoformulations or nanoproducts for the effective management of phytopathogens deterring the growth and yield of plants and thus the overall crop productivity. Zinc nanomaterials represent a versatile [...] Read more.

A facet of nanorenaissance in plant pathology hailed the research on the development and application of nanoformulations or nanoproducts for the effective management of phytopathogens deterring the growth and yield of plants and thus the overall crop productivity. Zinc nanomaterials represent a versatile class of nanoproducts and nanoenabled devices as these nanomaterials can be synthesized in quantum amounts through economically affordable processes/approaches. Further, these nanomaterials exhibit potential targeted antimicrobial properties and low to negligible phytotoxicity activities that well-qualify them to be applied directly or in a deviant manner to accomplish significant antibacterial, antimycotic, antiviral, and antitoxigenic activities against diverse phytopathogens causing plant diseases. The photo-catalytic, fluorescent, and electron generating aspects associated with zinc nanomaterials have been utilized for the development of sensor systems (optical and electrochemical biosensors), enabling quick, early, sensitive, and on-field assessment or quantification of the test phytopathogen. However, the proficient use of Zn-derived nanomaterials in the management of plant pathogenic diseases as nanopesticides and on-field sensor system demands that the associated eco- and biosafety concerns should be well discerned and effectively sorted beforehand. Current and possible utilization of zinc-based nanostructures in plant disease diagnosis and management and their safety in the agroecosystem is highlighted. Full article

(This article belongs to the Special Issue Fungal Nanotechnology)

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