Research

12 pages, 1596 KiB

Open AccessArticle

Ectomycorrhizal Fungal Community and Ascoma Production in a Declining Tuber borchii Plantation

by Francesca Ori, Marco Leonardi, Federico Puliga, Enrico Lancellotti, Giovanni Pacioni, Mirco Iotti and Alessandra Zambonelli

J. Fungi 2023, 9(6), 678; https://doi.org/10.3390/jof9060678 - 15 Jun 2023

Cited by 1 | Viewed by 2089

Abstract

Tuber borchii is an edible ectomycorrhizal mushroom of considerable economic value. Its cultivation has become popular in recent years, but there are few studies on the factors affecting its productivity. In this work, the ascoma production and the ectomycorrhizal (ECM) community of a [...] Read more.

Tuber borchii is an edible ectomycorrhizal mushroom of considerable economic value. Its cultivation has become popular in recent years, but there are few studies on the factors affecting its productivity. In this work, the ascoma production and the ectomycorrhizal (ECM) community of a T. borchii plantation, established in an intensive farming area where this truffle is not naturally present, were studied. Tuber borchii production drastically declined from 2016 to 2021, and ascomata of other Tuber species (T. maculatum and T. rufum) were found from 2017. Molecular characterization of ectomycorrhizae carried out in 2016 identified 21 ECM fungal species, of which T. maculatum (22%) and Tomentella coerulea (19%) were the most abundant. Tuber borchii ectomycorrizae (16%) were almost entirely confined to the fruiting points. The diversity and structure of the ECM community on Pinus pinea were significantly different from those observed on hardwood trees. The obtained results suggest that T. maculatum (a native of the study site) tends to replace T. borchii through a mechanism of competitive exclusion. Although T. borchii cultivation is possible in suboptimal environments, particular care should be taken to limit competition with ECM fungi more suitable for local conditions. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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16 pages, 2829 KiB

Open AccessArticle

Beech Leaf Disease Severity Affects Ectomycorrhizal Colonization and Fungal Taxa Composition

by Claudia Bashian-Victoroff, Alexis Brown, Andrew L. Loyd, Sarah R. Carrino-Kyker and David J. Burke

J. Fungi 2023, 9(4), 497; https://doi.org/10.3390/jof9040497 - 21 Apr 2023

Cited by 2 | Viewed by 2297

Abstract

Beech leaf disease (BLD) is an emerging forest infestation affecting beech trees (Fagus spp.) in the midwestern and northeastern United States and southeastern Canada. BLD is attributed to the newly recognized nematode Litylenchus crenatae subsp. mccannii. First described in Lake County, [...] Read more.

Beech leaf disease (BLD) is an emerging forest infestation affecting beech trees (Fagus spp.) in the midwestern and northeastern United States and southeastern Canada. BLD is attributed to the newly recognized nematode Litylenchus crenatae subsp. mccannii. First described in Lake County, Ohio, BLD leads to the disfigurement of leaves, canopy loss, and eventual tree mortality. Canopy loss limits photosynthetic capacity, likely impacting tree allocation to belowground carbon storage. Ectomycorrhizal fungi are root symbionts, which rely on the photosynthesis of autotrophs for nutrition and growth. Because BLD limits tree photosynthetic capacity, ECM fungi may receive less carbohydrates when associating with severely affected trees compared with trees without BLD symptoms. We sampled root fragments from cultivated F. grandifolia sourced from two provenances (Michigan and Maine) at two timepoints (fall 2020 and spring 2021) to test whether BLD symptom severity alters colonization by ectomycorrhizal fungi and fungal community composition. The studied trees are part of a long-term beech bark disease resistance plantation at the Holden Arboretum. We sampled from replicates across three levels of BLD symptom severity and compared fungal colonization via visual scoring of ectomycorrhizal root tip abundance. Effects of BLD on fungal communities were determined through high-throughput sequencing. We found that ectomycorrhizal root tip abundance was significantly reduced on the roots of individuals of the poor canopy condition resulting from BLD, but only in the fall 2020 collection. We found significantly more ectomycorrhizal root tips from root fragments collected in fall 2020 than in spring 2021, suggesting a seasonal effect. Community composition of ectomycorrhizal fungi was not impacted by tree condition but did vary between provenances. We found significant species level responses of ectomycorrhizal fungi between levels of both provenance and tree condition. Of the taxa analyzed, two zOTUs had significantly lower abundance in high-symptomatology trees compared with low-symptomatology trees. These results provide the first indication of a belowground effect of BLD on ectomycorrhizal fungi and contribute further evidence to the role of these root symbionts in studies of tree disease and forest pathology. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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13 pages, 1953 KiB

Open AccessArticle

Assessing the Effect of Slope Position on the Community Assemblage of Soil Diazotrophs and Root Arbuscular Mycorrhizal Fungi

by Dan Xiao, Tao Hong, Meifeng Chen, Xunyang He and Kelin Wang

J. Fungi 2023, 9(4), 394; https://doi.org/10.3390/jof9040394 - 23 Mar 2023

Cited by 3 | Viewed by 1100

Abstract

Considering the crucial role of soil diazotrophs and root arbuscular mycorrhizal fungi (AMF) in soil nutrient cycling during ecosystem restoration, diazotroph and AMF communities may be determined by slope position. However, the effect of slope position on diazotroph and AMF abundance, diversity, and [...] Read more.

Considering the crucial role of soil diazotrophs and root arbuscular mycorrhizal fungi (AMF) in soil nutrient cycling during ecosystem restoration, diazotroph and AMF communities may be determined by slope position. However, the effect of slope position on diazotroph and AMF abundance, diversity, and community composition of karst ecosystems remains unknown. In this study, soil diazotrophs and root AMF characteristics on varying slope positions were assessed in a karst shrub ecosystem. The results displayed that the abundance of soil diazotrophs and root AMF diversity were significantly affected by slope position. Diazotroph abundance accompanied by soil nutrient and plant richness was higher on the lower slopes than the upper slopes, whereas root AMF diversity displayed the opposite trend. The soil diazotroph and root AMF community composition differed among the upper, middle, and lower slopes. The dominant taxa of soil diazotrophs and root AMF at the order level were Rhizobiales and Glomerales, respectively. Moreover, the diazotroph order of Nostocales and the AMF order of Paraglomerales were richer on the upper slopes than on the lower slopes. The slope position directly affected the plant diversity and soil nutrient distribution, indirectly affecting the diazotroph and AMF communities. Increased available nitrogen on the lower slope caused great diazotroph abundance by stimulating plant growth with sufficient carbohydrates. However, low soil nutrients and plant diversity but high plant root biomass induced more root AMF diversity on the upper slope than on the lower slope. Therefore, this study expands the knowledge of soil diazotroph and root AMF ecological functions along different slope positions during vegetation recovery for the successive stages of grass and shrub in the karst region. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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14 pages, 36403 KiB

Open AccessArticle

Plant Growth Promoting Bacteria and Arbuscular Mycorrhizae Improve the Growth of Persea americana var. Zutano under Salt Stress Conditions

by Richard Solórzano-Acosta, Marcia Toro and Doris Zúñiga-Dávila

J. Fungi 2023, 9(2), 233; https://doi.org/10.3390/jof9020233 - 10 Feb 2023

Cited by 4 | Viewed by 1962

Abstract

In Peru, almost 50% of the national agricultural products come from the coast, highlighting the production of avocado. Much of this area has saline soils. Beneficial microorganisms can favorably contribute to mitigating the effect of salinity on crops. Two trials were carried out [...] Read more.

In Peru, almost 50% of the national agricultural products come from the coast, highlighting the production of avocado. Much of this area has saline soils. Beneficial microorganisms can favorably contribute to mitigating the effect of salinity on crops. Two trials were carried out with var. Zutano to evaluate the role of native rhizobacteria and two Glomeromycota fungi, one from a fallow (GFI) and the other from a saline soil (GWI), in mitigating salinity in avocado: (i) the effect of plant growth promoting rhizobacteria, and (ii) the effect of inoculation with mycorrhizal fungi on salt stress tolerance. Rhizobacteria P. plecoglissicida, and B. subtilis contributed to decrease the accumulation of chlorine, potassium and sodium in roots, compared to the uninoculated control, while contributing to the accumulation of potassium in the leaves. Mycorrhizae increased the accumulation of sodium, potassium, and chlorine ions in the leaves at a low saline level. GWI decreased the accumulation of sodium in the leaves compared to the control (1.5 g NaCl without mycorrhizae) and was more efficient than GFI in increasing the accumulation of potassium in leaves and reducing chlorine root accumulation. The beneficial microorganisms tested are promising in the mitigation of salt stress in avocado. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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17 pages, 2929 KiB

Open AccessArticle

Beta Diversity of Arbuscular Mycorrhizal Communities Increases in Time after Crop Establishment of Peruvian Sacha Inchi (Plukenetia volubilis)

by Ana Maria de la Sota Ricaldi, Sofía Rengifo del Águila, Raúl Blas Sevillano, Álvaro López-García and Mike Anderson Corazon-Guivin

J. Fungi 2023, 9(2), 194; https://doi.org/10.3390/jof9020194 - 02 Feb 2023

Cited by 2 | Viewed by 1726

Abstract

(1) Background: Beta diversity, i.e., the variance in species compositions across communities, has been pointed out as a main factor for explaining ecosystem functioning. However, few studies have directly tested the effect of crop establishment on beta diversity. We studied beta diversity patterns [...] Read more.

(1) Background: Beta diversity, i.e., the variance in species compositions across communities, has been pointed out as a main factor for explaining ecosystem functioning. However, few studies have directly tested the effect of crop establishment on beta diversity. We studied beta diversity patterns of arbuscular mycorrhizal (AM) fungal communities associated to sacha inchi (Plukenetia volubilis) after crop establishment. (2) Methods: We molecularly characterized the AM fungal communities associated to roots of sacha inchi in plots after different times of crop establishment, from less than one year to older than three. We analyzed the patterns of alpha, beta, and phylogenetic diversity, and the sources of variation of AM fungal community composition. (3) Results: Beta diversity increased in the older plots, but no temporal effect in alpha or phylogenetic diversity was found. The AM fungal community composition was driven by environmental factors (altitude and soil conditions). A part of this variation could be attributed to differences between sampled locations (expressed as geographic coordinates). Crop age, in turn, affected the composition with no interactions with the environmental conditions or spatial location. (4) Conclusions: These results point out towards a certain recovery of the soil microbiota after sacha inchi establishment. This fact could be attributed to the low-impact management associated to this tropical crop. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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14 pages, 2430 KiB

Open AccessArticle

The Potential of Ectomycorrhizal Fungi to Modulate below and Aboveground Communities May Be Mediated by 1-Octen-3-ol

by Inês Ferreira, Teresa Dias and Cristina Cruz

J. Fungi 2023, 9(2), 180; https://doi.org/10.3390/jof9020180 - 29 Jan 2023

Cited by 4 | Viewed by 1448

Abstract

It is known that ectomycorrhizal (ECM) fungi can modulate below and aboveground communities. They are a key part of belowground communication as they produce a vast array of metabolites, including volatile organic compounds (VOCs) such as 1-octen-3-ol. Here, we tested if the VOC [...] Read more.

It is known that ectomycorrhizal (ECM) fungi can modulate below and aboveground communities. They are a key part of belowground communication as they produce a vast array of metabolites, including volatile organic compounds (VOCs) such as 1-octen-3-ol. Here, we tested if the VOC 1-octen-3-ol may be involved in the ECM fungal mechanisms that modulate below and aboveground communities. For that, we conducted three in vitro assays with ECM fungi and the 1-octen-3-ol volatile to (i) explore the effects of mycelium growth of three ECM species, (ii) investigate the impact on the germination of six host Cistaceae species, and (iii) study the impact on host plant traits. The effects of 1-octen-3-ol on mycelium growth of the three ECM species depended on the dose and species: Boletus reticulatus was the most sensitive species to the low (VOC) dose, while T. leptoderma was the most tolerant. In general, the presence of the ECM fungi resulted in higher seed germination, while 1-octen-3-ol resulted in lower seed germination. The combined application of the ECM fungus and the volatile further inhibited seed germination, possibly due to the accumulation of 1-octen-3-ol above the plant species’ threshold. Seed germination and plant development of Cistaceae species were influenced by ECM fungal volatiles, suggesting that 1-octen-3-ol may mediate changes in below and aboveground communities. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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15 pages, 22248 KiB

Open AccessArticle

Effects of Arbuscular Mycorrhizal Fungi on the Growth and Root Cell Ultrastructure of Eucalyptus grandis under Cadmium Stress

by Yuxuan Kuang, Xue Li, Zhihao Wang, Xinyang Wang, Hongjian Wei, Hui Chen, Wentao Hu and Ming Tang

J. Fungi 2023, 9(2), 140; https://doi.org/10.3390/jof9020140 - 19 Jan 2023

Cited by 7 | Viewed by 1760

Abstract

Eucalyptus grandis (E. grandis) has been reported to form a symbiosis with arbuscular mycorrhizal fungi (AMF), which plays an important role in improving plant tolerance of heavy metal. However, the mechanism of how AMF intercept and transport cadmium (Cd) at [...] Read more.

Eucalyptus grandis (E. grandis) has been reported to form a symbiosis with arbuscular mycorrhizal fungi (AMF), which plays an important role in improving plant tolerance of heavy metal. However, the mechanism of how AMF intercept and transport cadmium (Cd) at the subcellular level in E. grandis still remains to be researched. In this study, a pot experiment was conducted to investigate the growth performance of E. grandis under Cd stress and Cd absorption resistance of AMF and explored the Cd localization in the root by using transmission electron microscopy and energy dispersive X-ray spectroscopy. The results showed that AMF colonization could enhance plant growth and photosynthetic efficiency of E. grandis and reduce the translocation factor of Cd under Cd stress. After being treated with 50, 150, 300, and 500 μM Cd, the translocation factor of Cd in E. grandis with AMF colonization decreased by 56.41%, 62.89%, 66.67%, and 42.79%, respectively. However, the mycorrhizal efficiency was significant only at low Cd concentrations (50, 150, and 300 μM). Under 500 μM Cd concentration condition, the colonization of AMF in roots decreased, and the alleviating effect of AMF was not significant. Ultrastructural observations showed that Cd is abundant in regular lumps and strips in the cross-section of E. grandis root cell. AMF protected plant cells by retaining Cd in the fungal structure. Our results suggested that AMF alleviated Cd toxicity by regulating plant physiology and altering the distribution of Cd in different cell sites. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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18 pages, 4476 KiB

Open AccessArticle

The Transcriptional Responses of Ectomycorrhizal Fungus, Cenococcum geophilum, to Drought Stress

by Mingtao Li, Chao Yuan, Xiaohui Zhang, Wenbo Pang, Panpan Zhang, Rongzhang Xie, Chunlan Lian and Taoxiang Zhang

J. Fungi 2023, 9(1), 15; https://doi.org/10.3390/jof9010015 - 21 Dec 2022

Cited by 6 | Viewed by 1646

Abstract

With global warming, drought has become one of the major environmental pressures that threaten the development of global agricultural and forestry production. Cenococcum geophilum (C. geophilum) is one of the most common ectomycorrhizal fungi in nature, which can form mycorrhiza with [...] Read more.

With global warming, drought has become one of the major environmental pressures that threaten the development of global agricultural and forestry production. Cenococcum geophilum (C. geophilum) is one of the most common ectomycorrhizal fungi in nature, which can form mycorrhiza with a large variety of host trees of more than 200 tree species from 40 genera of both angiosperms and gymnosperms. In this study, six C. geophilum strains with different drought tolerance were selected to analyze their molecular responses to drought stress with treatment of 10% polyethylene glycol. Our results showed that drought-sensitive strains absorbed Na and K ions to regulate osmotic pressure and up-regulated peroxisome pathway genes to promote the activity of antioxidant enzymes to alleviate drought stress. However, drought-tolerant strains responded to drought stress by up-regulating the functional genes involved in the ubiquinone and other terpenoid-quinone biosynthesis and sphingolipid metabolism pathways. The results provided a foundation for studying the mechanism of C. geophilum response to drought stress. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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15 pages, 1707 KiB

Open AccessArticle

Effects of Arbuscular Mycorrhizal Fungi and Biochar on Growth, Nutrient Absorption, and Physiological Properties of Maize (Zea mays L.)

by Jiahua Sun, Qiong Jia, Yi Li, Ting Zhang, Jiayuan Chen, Yanan Ren, Kanglong Dong, Shuai Xu, Nan-Nan Shi and Shenglei Fu

J. Fungi 2022, 8(12), 1275; https://doi.org/10.3390/jof8121275 - 05 Dec 2022

Cited by 35 | Viewed by 3511

Abstract

Arbuscular mycorrhizal fungi (AMFs) and biochar are two common alternatives to chemical fertilizers applied to soil to improve crop growth. However, their interactive effects on maize (Zea mays L.) growth, nutrient absorption, and physiological properties remain poorly understood. In this study, maize [...] Read more.

Arbuscular mycorrhizal fungi (AMFs) and biochar are two common alternatives to chemical fertilizers applied to soil to improve crop growth. However, their interactive effects on maize (Zea mays L.) growth, nutrient absorption, and physiological properties remain poorly understood. In this study, maize plants were grown in pots treated with biochar and AMFs Diversispora eburnea, alone or in combination. The results showed that the individual application of AMFs or biochar increased maize growth and mineral contents in shoots and roots (including P, K, Ca, Na, Mg, Fe, Mn, and Zn). The chlorophyll a, chlorophyll b, and total chlorophyll contents in AMF-treated leaves were significantly higher than those in the control treatment group. However, AMFs had no synergistic effects with biochar on maize growth, nutrient absorption, nor photosynthetic pigments. The application of biochar to the soil significantly reduced mycorrhizal colonization by 40.58% in the root tissues, accompanied by a significant decline in mycorrhizal dependency from 80.57% to −28.67%. We conclude that the application of biochar and AMFs can affect maize growth, nutrient uptake, and physiological properties. Our study can provide vital information for further resource use optimization in agroecosystems. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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14 pages, 1859 KiB

Open AccessArticle

Impacts of Biogas Slurry Fertilization on Arbuscular Mycorrhizal Fungal Communities in the Rhizospheric Soil of Poplar Plantations

by Xing-Ye Yu, Bao-Teng Wang, Long Jin, Hong-Hua Ruan, Hyung-Gwan Lee and Feng-Jie Jin

J. Fungi 2022, 8(12), 1253; https://doi.org/10.3390/jof8121253 - 27 Nov 2022

Cited by 1 | Viewed by 1346

Abstract

The majority of terrestrial plants are symbiotic with arbuscular mycorrhizal fungi (AMF). Plants supply carbohydrates to microbes, whereas AMF provide plants with water and other necessary nutrients—most typically, phosphorus. Understanding the response of the AMF community structure to biogas slurry (BS) fertilization is [...] Read more.

The majority of terrestrial plants are symbiotic with arbuscular mycorrhizal fungi (AMF). Plants supply carbohydrates to microbes, whereas AMF provide plants with water and other necessary nutrients—most typically, phosphorus. Understanding the response of the AMF community structure to biogas slurry (BS) fertilization is of great significance for sustainable forest management. This study aimed to look into the effects of BS fertilization at different concentrations on AMF community structures in rhizospheric soil in poplar plantations. We found that different fertilization concentrations dramatically affected the diversity of AMF in the rhizospheric soil of the poplar plantations, and the treatment with a high BS concentration showed the highest Shannon diversity of AMF and OTU richness (Chao1). Further analyses revealed that Glomerales, as the predominant order, accounted for 36.2–42.7% of the AMF communities, and the relative abundance of Glomerales exhibited negligible changes with different BS fertilization concentrations, whereas the order Paraglomerales increased significantly in both the low- and high-concentration treatments in comparison with the control. Furthermore, the addition of BS drastically enhanced the relative abundance of the dominant genera, Glomus and Paraglomus. The application of BS could also distinguish the AMF community composition in the rhizospheric soil well. An RDA analysis indicated that the dominant genus Glomus was significantly positively correlated with nitrate reductase activity, while Paraglomus showed a significant positive correlation with available P. Overall, the findings suggest that adding BS fertilizer to poplar plantations can elevate the diversity of AMF communities in rhizospheric soil and the relative abundance of some critical genera that affect plant nutrient uptake. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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13 pages, 2282 KiB

Open AccessArticle

Symbiotic Fungi Alter the Acquisition of Phosphorus in Camellia oleifera through Regulating Root Architecture, Plant Phosphate Transporter Gene Expressions and Soil Phosphatase Activities

by Ming-Ao Cao, Rui-Cheng Liu, Zhi-Yan Xiao, Abeer Hashem, Elsayed Fathi Abd_Allah, Mashail Fahad Alsayed, Wiwiek Harsonowati and Qiang-Sheng Wu

J. Fungi 2022, 8(8), 800; https://doi.org/10.3390/jof8080800 - 29 Jul 2022

Cited by 12 | Viewed by 1927

Abstract

Plant roots can be colonized by many symbiotic fungi, whereas it is unclear whether and how symbiotic fungi including arbuscular mycorrhizal fungi and endophytic fungi promote phosphorus (P) uptake in Camellia oleifera plants. The objective of the present study was to analyze the [...] Read more.

Plant roots can be colonized by many symbiotic fungi, whereas it is unclear whether and how symbiotic fungi including arbuscular mycorrhizal fungi and endophytic fungi promote phosphorus (P) uptake in Camellia oleifera plants. The objective of the present study was to analyze the effect of inoculation with a culturable endophytic fungus (Piriformospora indica), three arbuscular mycorrhizal fungi (Funneliformis mosseae, Diversispora versiformis, and Rhizophagus intraradices), and mixture of F. mosseae, D. versiformis and R. intraradices on plant growth, root architecture, soil Olsen-P, soil phosphatase activities, leaf and root P concentrations, and phosphate transporter gene expressions, in order to explore the potential and mechanism of these symbiotic fungi on P acquisition. All the symbiotic fungi colonized roots of C. oleifera after 16 weeks, with P. indica showing the best effect on fungal colonization. All the symbiotic fungi significantly increased acid, neutral, and total phosphatase activities in the soil, accompanied with an elevation of soil Olsen-P, of which P. indica presented the best effect. All symbiotic fungal treatments, except D. versiformis, significantly promoted plant growth, coupled with an increase in root total length, area, and volume. Symbiotic fungi almost up-regulated root CoPHO1-3 expressions as well as leaf CoPHO1-1, CoPHO1-3, and CoPHT1;4 expressions. Correlation analysis showed that P concentrations in leaves and roots were significantly positively correlated with root morphological variables (length, volume, and surface area) and soil acid, neutral and total phosphatase activities. It is concluded that symbiotic fungi, especially P. indica, played an important role in P uptake of C. oleifera plants through regulating root architecture, part plant phosphate transporter gene expressions and soil phosphatase activities. Full article

(This article belongs to the Special Issue Friends of Plants: Mycorrhizal Fungi)

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