Fusarium Species in Agriculture Industry

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungal Evolution, Biodiversity and Systematics".

Deadline for manuscript submissions: closed (31 October 2022) | Viewed by 10474

Special Issue Editor


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Guest Editor
Department of Plant Medicine, National Pingtung University of Science and Technology, Pingtung 912301, Taiwan
Interests: molecular diagnosis; phytopathology; biological control; disease management; plasma sterilization technology
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Special Issue Information

Dear Colleagues,

Fusarium spp. and Colletotrichum spp. are ubiquitous pathogens that cause diseases in a wide range of crops, which makes them one of the most devastating and dangerous phytopathogens worldwide. The conventional methods for the diagnosis of fungal crop diseases mostly depend on the evident symptoms and/or the isolation, culturing, and morphological observations of the causal fungal agent, as well as their pathogenicity and biochemical tests. Precise diagnosis is thus sometimes difficult, particularly when symptoms are not typical and clear. Advanced detection methods should be improved in terms of throughput, accuracy, user-friendliness, and cost-effectiveness, which should allow their use to emerge as an essential control strategy for the management of diseases caused by Fusarium spp. and Colletotrichum spp.

This Special Issue, entitled “Fusarium Species in Agriculture Industry”, aims to highlight current research on all aspects of Fusarium and Colletotrichum species. Experts specifically from the fields of molecular identification, molecular detection, molecular diagnosis, on-site detection, non-chemical control, and chemical control are invited to contribute their newest findings in the form of research articles, communications, or review articles, to provide a common platform for scientific communication, to deliver reliable information and to advance our understanding of the basis of these two important groups of fungi.

Dr. Ying-Hong Lin
Guest Editor

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Keywords

  • precise diagnosis
  • molecular identification
  • molecular detection
  • molecular diagnosis
  • on-site detection
  • non-chemical control
  • chemical control

Published Papers (4 papers)

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13 pages, 3533 KiB  
Article
Molecular Diagnosis and Vegetative Compatibility Group Analysis of Fusarium Wilt of Banana in Nepal
by Bimala Pant, Tingting Bai, Chanjuan Du, Suraj Baidya, Prem Bahadur Magar, Shrinkhala Manandhar, Jiban Shrestha, Miguel Dita, Mathieu Rouard, Gang Fu and Si-Jun Zheng
J. Fungi 2023, 9(2), 208; https://doi.org/10.3390/jof9020208 - 05 Feb 2023
Cited by 2 | Viewed by 2685
Abstract
Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. [...] Read more.
Fusarium wilt of banana (FWB), caused by Fusarium oxysporum f. sp. cubense (Foc), is the most important constraint of the banana industry globally. In Nepal, epidemics resembling FWB have been increasingly observed on the Malbhog cultivar in the past several years. However, the disease has not been officially reported yet, and consequently, little is known about the pathogen present across the country. In this study, we characterized 13 fungal strains isolated from banana plants of the Malbhog cultivar (Silk, AAB) showing symptoms similar to FWB in banana plantations in Nepal. All of the strains were typed as belonging to the F. oxysporum and caused FWB symptoms when inoculated in the Malbhog and Cachaco (Bluggoe, ABB) cultivars. No symptoms were observed in the Williams cultivar (Cavendish, AAA). Vegetative compatibility group (VCG) analysis classified the strains as VCG 0124 or VCG 0125. PCR analyses conducted with primers specific for Foc race 1 (Foc R1) or Foc tropical race 4 (TR4) revealed that all the strains reacted positively for Foc R1 and none for TR4. Altogether, our results demonstrated that the pathogen populations causing FWB of the Malbhog cultivar in Nepal were Foc R1. This work reported, for the first time, the occurrence of FWB in Nepal. Further studies with larger Foc populations are needed to better understand disease epidemiology to design sustainable disease management strategies. Full article
(This article belongs to the Special Issue Fusarium Species in Agriculture Industry)
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17 pages, 4303 KiB  
Article
Antagonistic Effect of Trichoderma longibrachiatum (TL6 and TL13) on Fusarium solani and Fusarium avenaceum Causing Root Rot on Snow Pea Plants
by Thomas Afriyie Boakye, Huixia Li, Richard Osei, Solomon Boamah, Zhang Min, Chunhui Ni, Jin Wu, Mingming Shi and Wanqiang Qiao
J. Fungi 2022, 8(11), 1148; https://doi.org/10.3390/jof8111148 - 29 Oct 2022
Cited by 2 | Viewed by 1983
Abstract
Snow pea root rot in China is caused by Fusarium solani (FSH) and Fusarium avenaceum (FAH), which affect snow pea production. The chemical control methods used against FSH and FAH are toxic to the environment and resistance may be developed in persistence applications. [...] Read more.
Snow pea root rot in China is caused by Fusarium solani (FSH) and Fusarium avenaceum (FAH), which affect snow pea production. The chemical control methods used against FSH and FAH are toxic to the environment and resistance may be developed in persistence applications. Therefore, an alternative approach is needed to control these pathogens. This study focuses on Trichoderma longibrachiatum strains (TL6 and TL13), mycoparasitic mechanisms of FSH and FAH, as well as growth-promoting potentials on snow pea seedlings under FSH and FAH stress at the physiological, biochemical, and molecular levels. The average inhibitory rates of TL6 against FSH and FAH were 54.58% and 69.16%, respectively, on day 7. Similarly, TL13 average inhibitory rates against FSH and FAH were 59.06% and 71.27%, respectively, on day 7. The combined TL13 and TL6 with FSH and FAH reduced disease severity by 86.6, 81.6, 57.60, and 60.90%, respectively, in comparison to the controls. The snow pea plants inoculated with FSH and FAH without TL6 and TL13 increased malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents in the leaves by 64.8, 66.0, 64.4 and 65.9%, respectively, compared to the control. However, the combined FSH and FAH with TL6 and TL13 decreased the MDA and H2O2 content by 75.6, 76.8, 70.0, and 76.4%, respectively, in comparison to the controls. In addition, the combined TL6 + FSH and TL6 + FAH increased the activity of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) by 60.5, 64.7, and 60.3%, respectively, and 60.0, 64.9, and 56.6%, respectively, compared to the controls. Again, compared to the controls, the combined TL13 + FSH and TL13 + FAH increased the activity of SOD, POD, and CAT by 69.7, 68.6, and 65.6%, respectively, and 70.10, 69.5, and 65.8%, respectively. Our results suggest that the pretreatment of snow pea seeds with TL6 and TL13 increases snow pea seedling growth, controls FSH and FAH root rot, increases antioxidant enzyme activity, and activates plant defense mechanisms. The TL13 strain had the greatest performance in terms of pathogen inhibition and snow pea growth promotion compared to the TL6 strain. Full article
(This article belongs to the Special Issue Fusarium Species in Agriculture Industry)
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18 pages, 8227 KiB  
Article
Fusarium and Neocosmospora Species Associated with Rot of Cactaceae and Other Succulent Plants
by Sahar Kamali-Sarvestani, Reza Mostowfizadeh-Ghalamfarsa, Fatemeh Salmaninezhad and Santa Olga Cacciola
J. Fungi 2022, 8(4), 364; https://doi.org/10.3390/jof8040364 - 01 Apr 2022
Cited by 8 | Viewed by 4193
Abstract
Infections by Fusarium and Fusarium-like species on cacti and other succulent plants cause the syndrome known as Fusarium dry rot and soft rot. There are only few records of Fusarium species as pathogens of cacti and other succulent plants from Iran. The [...] Read more.
Infections by Fusarium and Fusarium-like species on cacti and other succulent plants cause the syndrome known as Fusarium dry rot and soft rot. There are only few records of Fusarium species as pathogens of cacti and other succulent plants from Iran. The objective of this study was the identification and characterization of fusarioid species recovered from ornamental succulents in Shiraz County, Iran. Three fusarioid species, including F. oxysporum, F. proliferatum, and Neocosmospora falciformis (formerly F. falciforme), were recovered from 29 diverse species of cacti and other succulents with symptoms of Fusarium dry rot and soft rot. The three fungal species were identified on the basis of morphological characters and the phylogenetic analysis of the translation elongation factor1-α (tef1) nuclear gene. The F. oxysporum isolates were identified as F. oxysporum f. sp. opuntiarum. The pathogenicity of the three fusarioid species was tested on a range of economically important ornamental succulents, mostly in the Cactaceae family. The three species showed a broad host spectrum and induced different types of symptoms on inoculated plants, including soft and dry rot, chlorosis, necrotic spots, wilt, drying, root and crown rot. This is the first report of N. falciformis as a pathogen of succulent plants worldwide. Full article
(This article belongs to the Special Issue Fusarium Species in Agriculture Industry)
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9 pages, 1527 KiB  
Brief Report
The Retromer Subunit CfVps29 Is Involved in the Growth, Development, and Pathogenicity of Colletotrichum fructicola
by Sizheng Li, Xiya Li and He Li
J. Fungi 2022, 8(8), 835; https://doi.org/10.3390/jof8080835 - 10 Aug 2022
Viewed by 997
Abstract
Camellia oleifera is an edible oil tree species native to China. Anthracnose is a common disease of Ca. oleifera, which reduces the production of the trees and brings huge economic losses. We have previously identified the fungus Colletotrichum fructicola as the major [...] Read more.
Camellia oleifera is an edible oil tree species native to China. Anthracnose is a common disease of Ca. oleifera, which reduces the production of the trees and brings huge economic losses. We have previously identified the fungus Colletotrichum fructicola as the major pathogen of anthracnose in Ca. oleifera. The retromer complex participates in the intracellular retrograde transport of the cargos from the endosome to the trans-Golgi network in the eukaryotes. Vacuolar protein sorting 29 is a subunit of the retromer complex. Targeted CfVPS29 gene deletion revealed that CfVps29 is involved in growth, conidiation, and the response to cell wall stress. We further found that the ΔCfvps29 mutant was minimally pathogenic to Ca. oleifera leaves, as a result of its defect in appressorium formation. This study illustrated the crucial functions of CfVps29 in the development, cell wall stress response, and pathogenicity of C. fructicola and, therefore, identified it as a potential fungicide target for the control of anthracnose. Full article
(This article belongs to the Special Issue Fusarium Species in Agriculture Industry)
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