Research

12 pages, 1994 KiB

Open AccessFeature PaperArticle

Functional Characterization of Pheromone Receptors in the Beet Webworm, Loxostege sticticalis (Lepidoptera: Pyralidae)

by Yu Zhang, Hai-Bin Han, Yan-Yan Li, Lin-Bo Xu, Li-Fen Hao, Hui Wang, Wen-He Wang, Shu-Jing Gao and Ke-Jian Lin

Insects 2023, 14(7), 584; https://doi.org/10.3390/insects14070584 - 27 Jun 2023

Viewed by 1076

Abstract

Lepidopteran insects mainly rely on sex pheromones to complete sexual communications. Pheromone receptors (PRs) are expressed on the olfactory receptor neurons (ORNs) of the sensilla trichodea and play an essential role in sexual communication. Despite extensive investigations into the mechanisms of peripheral recognition [...] Read more.

Lepidopteran insects mainly rely on sex pheromones to complete sexual communications. Pheromone receptors (PRs) are expressed on the olfactory receptor neurons (ORNs) of the sensilla trichodea and play an essential role in sexual communication. Despite extensive investigations into the mechanisms of peripheral recognition of sex pheromones in Lepidoptera, knowledge about these mechanisms in L. sticticalis remains limited. In this study, five candidate LstiPRs were analyzed in a phylogenetic tree with those of other Lepidopteran insects. Electroantennography (EAG) assays showed that the major sex pheromone component E11-14:OAc elicited a stronger antennal response than other compounds in male moths. Moreover, two types of neurons in sensilla trichodea were classified by single sensillum recordings, of which the “a” neuron specifically responded to E11-14:OAc. Five candidate PRs were functionally assayed by the heterologous expression system of Xenopus oocytes, and LstiPR2 responded to the major sex pheromone E11-14:OAc. Our findings suggest that LstiPR2 is a PR sensitive to L. sticticalis’s major sex pheromone compound, E11-14:OAc. Furthermore, this study offers valuable insights into the sexual communication behavior of L. sticticalis, forming a foundation for further analysis of the species’ central nervous system. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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13 pages, 6235 KiB

Open AccessArticle

Identification and Functional Characterization of General Odorant Binding Proteins in Orthaga achatina

by Yu Ma, Yu Li, Zhi-Qiang Wei, Jing-Hao Hou, Yu-Xiao Si, Jin Zhang, Shuang-Lin Dong and Qi Yan

Insects 2023, 14(3), 216; https://doi.org/10.3390/insects14030216 - 22 Feb 2023

Cited by 3 | Viewed by 1586

Abstract

The olfactory system in insects are crucial for recognition of host plants and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in detecting odorants released by host plants. Orthaga achatina (Lepidoptera: Pyralidae) is one of the most serious pests [...] Read more.

The olfactory system in insects are crucial for recognition of host plants and oviposition sites. General odorant binding proteins (GOBPs) are thought to be involved in detecting odorants released by host plants. Orthaga achatina (Lepidoptera: Pyralidae) is one of the most serious pests of camphor trees, Cinnamomum camphora (L.) Presl, an important urban tree species in southern China. In this study, we study the GOBPs of O. achatina. Firstly, two full-length GOBP genes (OachGOBP1 and OachGOBP2) were successfully cloned according to transcriptome sequencing results, and real-time quantitative PCR measurements showed that both GOBP genes were specifically expressed in the antennae of both sexes, proposing their important roles in olfaction. Then, both GOBP genes were heterologous expressed in Escherichia coli and fluorescence competitive binding assays were conducted. The results showed that OachGOBP1 could bind Farnesol (K_i = 9.49 μM) and Z11-16: OH (K_i = 1.57 μM). OachGOBP2 has a high binding affinity with two camphor plant volatiles (Farnesol, K_i = 7.33 μM; α-Phellandrene, K_i = 8.71 μM) and two sex pheromone components (Z11-16: OAc, K_i = 2.84 μM; Z11-16: OH, K_i = 3.30 μM). These results indicate that OachGOBP1 and OachGOBP2 differ in terms of odorants and other ligands. Furthermore, key amino acid residues that bind to plant volatiles were identified in GOBPs using 3-D structure modeling and ligand molecular docking, predicting the interactions between the GOBPs and the host plant volatiles. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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16 pages, 3052 KiB

Open AccessFeature PaperArticle

Characterization of Two Aldehyde Oxidases from the Greater Wax Moth, Galleria mellonella Linnaeus. (Lepidoptera: Pyralidae) with Potential Role as Odorant-Degrading Enzymes

by Ricardo Godoy, Ignacio Arias, Herbert Venthur, Andrés Quiroz and Ana Mutis

Insects 2022, 13(12), 1143; https://doi.org/10.3390/insects13121143 - 12 Dec 2022

Cited by 4 | Viewed by 1543

Abstract

Odorant-degrading enzymes (ODEs) are proposed to degrade/inactivate volatile organic compounds (VOCs) on a millisecond timescale. Thus, ODEs play an important role in the insect olfactory system as a reset mechanism. The inhibition of these enzymes could incapacitate the olfactory system and, consequently, disrupt [...] Read more.

Odorant-degrading enzymes (ODEs) are proposed to degrade/inactivate volatile organic compounds (VOCs) on a millisecond timescale. Thus, ODEs play an important role in the insect olfactory system as a reset mechanism. The inhibition of these enzymes could incapacitate the olfactory system and, consequently, disrupt chemical communication, promoting and complementing the integrated pest management strategies. Here, we report two novel aldehyde oxidases, AOX-encoding genes GmelAOX2 and GmelAOX3, though transcriptomic analysis in the greater wax moth, Galleria mellonella. GmelAOX2 was clustered in a clade with ODE function, according to phylogenetic analysis. Likewise, to unravel the profile of volatiles that G. mellonella might face besides the sex pheromone blend, VOCs were trapped from honeycombs and the identification was made by gas chromatography–mass spectrometry. Semi-quantitative RT-PCR showed that GmelAXO2 has a sex-biased expression, and qRT-PCR indicated that both GmelAOX2 and GmelAOX3 have a higher relative expression in male antennae rather than female antennae. A functional assay revealed that antennal extracts had the strongest enzymatic activity against undecanal (4-fold) compared to benzaldehyde (control). Our data suggest that these enzymes have a crucial role in metabolizing sex pheromone compounds as well as plant-derived aldehydes, which are related to honeycombs and the life cycle of G. mellonella. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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18 pages, 6539 KiB

Open AccessArticle

Transcriptomic Identification and Expression Profile Analysis of Odorant-Degrading Enzymes from the Asian Corn Borer Moth, Ostrinia furnacalis

by Liya Zhang, Yidan Shen, Xingchuan Jiang and Su Liu

Insects 2022, 13(11), 1027; https://doi.org/10.3390/insects13111027 - 06 Nov 2022

Cited by 1 | Viewed by 1561

Abstract

The Asian corn borer moth Ostrinia furnacalis is an important lepidopteran pest of maize in Asia. Odorant-degrading enzymes (ODEs), including carboxylesterases (CCEs), glutathione S-transferases (GSTs), cytochrome P450s (CYPs), UDP-glycosyltransferases (UGTs), and aldehyde oxidases (AOXs), are responsible for rapid inactivation of odorant signals [...] Read more.

The Asian corn borer moth Ostrinia furnacalis is an important lepidopteran pest of maize in Asia. Odorant-degrading enzymes (ODEs), including carboxylesterases (CCEs), glutathione S-transferases (GSTs), cytochrome P450s (CYPs), UDP-glycosyltransferases (UGTs), and aldehyde oxidases (AOXs), are responsible for rapid inactivation of odorant signals in the insect antennae. In this study, we performed a transcriptome assembly for the antennae of O. furnacalis to identify putative ODE genes. Transcriptome sequencing revealed 35,056 unigenes, and 21,012 (59.94%) of these were annotated by searching against the reference sequences in the NCBI non-redundant (NR) protein database. For functional classification, these unigenes were subjected to Gene Ontology (GO), Eukaryotic Orthologous Groups (KOG), and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. We identified 79 genes encoding putative ODEs: 19 CCEs, 17 GSTs, 24 CYPs, 13 UGTs, and 6 AOXs. BLASTX best hit results indicated that these genes shared quite high amino acid identities with their respective orthologs from other lepidopteran species. Reverse transcription-quantitative PCR showed that OfurCCE2, OfurCCE5, and OfurCCE18 were enriched in male antennae, while OfurCCE7 and OfurCCE10 were enriched in female antennae. OfurCCE14 and OfurCCE15 were expressed at near-equal amounts in the antennae of both sexes. Our findings establish a solid foundation for future studies aimed at understanding the olfactory functions of these genes in O. furnacalis. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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16 pages, 2904 KiB

Open AccessArticle

Identification and Expression Analysis of Odorant-Binding and Chemosensory Protein Genes in Virus Vector Nephotettix cincticeps

by Xuefei Chang, Yaluan Bi, Haipeng Chi, Qi Fang, Zhaozhi Lu, Fang Wang and Gongyin Ye

Insects 2022, 13(11), 1024; https://doi.org/10.3390/insects13111024 - 05 Nov 2022

Cited by 1 | Viewed by 1433

Abstract

The insect odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the perception and discrimination of insects to host odor cues. Nephotettix cincticeps, one of the destructive pests of rice plants, not only directly damages hosts by sucking, but also [...] Read more.

The insect odorant binding proteins (OBPs) and chemosensory proteins (CSPs) are involved in the perception and discrimination of insects to host odor cues. Nephotettix cincticeps, one of the destructive pests of rice plants, not only directly damages hosts by sucking, but also indirectly transmits plant viruses in the field. Previous study found that two rice volatiles ((E)-β-caryophyllene and 2-heptanol) induced by rice dwarf virus (RDV) mediated the olfactory behavior of N. cincticeps, which may promote virus dispersal. However, the OBPs and CSPs in N. cincticeps are still unknown. In this study, to identify the OBP and CSP genes in N. cincticeps, transcriptomic analyses were performed. In total, 46,623 unigenes were obtained. Twenty putative OBP and 13 CSP genes were discovered and identified. Phylogenetic analyses revealed that five putative OBPs belonged to the plus-C OBP family, and the other classic OBPs and CSPs were distributed among other orthologous groups. A total of 12 OBP and 10 CSP genes were detected, and nine OBP and three CSP genes were highly expressed in N. cincticeps antennae compared with other tissues. This study, for the first time, provides a valuable resource to well understand the molecular mechanism of N. cincticeps in the perception and discrimination of the two volatiles induced by RDV infection. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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14 pages, 4237 KiB

Open AccessArticle

Foreleg Transcriptomic Analysis of the Chemosensory Gene Families in Plagiodera versicolora (Coleoptera: Chrysomelidae)

by Zheran Wu, Na Tong, Yang Li, Jinmeng Guo, Min Lu and Xiaolong Liu

Insects 2022, 13(9), 763; https://doi.org/10.3390/insects13090763 - 24 Aug 2022

Cited by 5 | Viewed by 1580

Abstract

Plagiodera versicolora (Coleoptera: Chrysomelidae) is a worldwide leaf-eating forest pest in salicaceous trees. The forelegs play important roles in the chemoreception of insects. In this study, we conducted a transcriptome analysis of adult forelegs in P. versicolora and identified a total of 53 [...] Read more.

Plagiodera versicolora (Coleoptera: Chrysomelidae) is a worldwide leaf-eating forest pest in salicaceous trees. The forelegs play important roles in the chemoreception of insects. In this study, we conducted a transcriptome analysis of adult forelegs in P. versicolora and identified a total of 53 candidate chemosensory genes encoding 4 chemosensory proteins (CSPs), 19 odorant binding proteins (OBPs), 10 odorant receptors (ORs), 10 gustatory receptors (GRs), 6 ionotropic receptors (IRs), and 4 sensory neuron membrane proteins (SNMPs). Compared with the previous antennae transcriptome data, 1 CSP, 4 OBPs, 1 OR, 3 IRs, and 4 GRs were newly identified in the forelegs. Subsequently, the tissue expression profiles of 10 P. versicolora chemosensory genes were performed by real-time quantitative PCR. The results showed that PverOBP25, PverOBP27, and PverCSP6 were highly expressed in the antennae of both sexes. PverCSP11 and PverIR9 are predominately expressed in the forelegs than in the antennae. In addition, the expression levels of PverGR15 in female antennae and forelegs were significantly higher than those in the male antennae, implying that it may be involved in some female-specific behaviors such as oviposition site seeking. This work would greatly further the understanding of the chemoreception mechanism in P. versicolora. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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17 pages, 6411 KiB

Open AccessArticle

Identification and Expression Analysis of Chemosensory Genes in the Antennal Transcriptome of Chrysanthemum Aphid Macrosiphoniella sanborni

by Jian Zhong, Yuxin Wang, Yufan Lu, Xiaoou Ma, Qian Zhang, Xiaoyue Wang, Qixiang Zhang and Ming Sun

Insects 2022, 13(7), 597; https://doi.org/10.3390/insects13070597 - 29 Jun 2022

Cited by 6 | Viewed by 1949

Abstract

As one of the most destructive oligophagous pests, the chrysanthemum aphid (Macrosiphoniella sanborni) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about [...] Read more.

As one of the most destructive oligophagous pests, the chrysanthemum aphid (Macrosiphoniella sanborni) has seriously restricted the sustainable development of the chrysanthemum industry. Olfaction plays a critical role in the environmental perception of aphids, but very little is currently known about the chemosensory mechanism of M. sanborni. In this study, four MsanOBPs, four MsanCSPs, eight MsanORs, two MsanIRs and one MsanSNMP were identified among the 28,323 unigenes derived from the antennal transcriptome bioinformatic analysis of M. sanborni adults. Then, comprehensive phylogenetic analyses of these olfactory-related proteins in different aphid species were performed using multiple sequence alignment. Subsequently, the odor-specific and wing-specific expression profiles of these candidate chemosensory genes were investigated using quantitative real-time PCR. The data showed that most of these chemosensory genes exhibited higher expression levels in alate aphids. Among them, MsanOBP9, MsanOR2, MsanOR4, MsanOR43b-1, MsanCSP1, MsanCSP2, MsanCSP4, MsanIR25a and MsanIR40a in alate aphids showed remarkably higher expression levels than in apterous aphids under the effect of the host plant volatiles, indicating that these genes may take part in the specific behaviors of alate adults, such as host recognition, oviposition site selection and so on. This study lays the groundwork for future research into the molecular mechanism of olfactory recognition in M. sanborni. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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12 pages, 11139 KiB

Open AccessArticle

Diversity and Molecular Evolution of Odorant Receptor in Hemipteran Insects

by Jiahui Tian, Youssef Dewer, Haoyuan Hu, Fengqi Li, Shiyong Yang and Chen Luo

Insects 2022, 13(2), 214; https://doi.org/10.3390/insects13020214 - 21 Feb 2022

Cited by 8 | Viewed by 2499

Abstract

Olfaction is a critical physiologic process for insects to interact with the environment, especially plant-emitted volatiles, during which odorant receptors (ORs) play an essential role in host recognition. Although OR gene evolution has been studied in many insect orders, a comprehensive evolutionary analysis [...] Read more.

Olfaction is a critical physiologic process for insects to interact with the environment, especially plant-emitted volatiles, during which odorant receptors (ORs) play an essential role in host recognition. Although OR gene evolution has been studied in many insect orders, a comprehensive evolutionary analysis and expression of OR gene gain and loss events among diverse hemipteran species are still required. In this study, we identified and analyzed 887 OR genes from 11 hemipteran species. The number of OR genes discovered in each species ranged from less than ten to hundreds. Phylogenetic analysis revealed that all identified Hemiptera OR genes were classified into seven major clades. Gene gain and loss events of OR have occurred in several species. Then, by positive selection, we discovered the amino acid differences between species to understand the molecular evolution of OR in the order Hemiptera. Additionally, we discussed how evolutionary analysis can aid the study of insect–plant communication. This study lays a foundation for subsequent investigations into the molecular mechanisms of Hemiptera olfactory receptors involved in host recognition. Full article

(This article belongs to the Special Issue Chemosensory Genes in Insects)

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