ijms-logo

Journal Browser

Journal Browser

Human Keratinocytes Stem Cells

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: closed (31 October 2022) | Viewed by 4342

Special Issue Editors

Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Zurich, Switzerland
Interests: human skin; keratinocytes; endothelial cells; skin substitutes; stem cells; melanocytes; adipose dervied stem cells; fat tissue; skin inflammation; immune cells; skin adipocyte progenitors

Special Issue Information

Dear Colleagues,

The epidermis is the outermost skin layer that protects the body from the outer environment. The epidermis of the skin constantly renews itself throughout adult life, and skin appendages such as hair follicles undergo a perpetual cycle of growth and degeneration. Stem cells and their progeny are responsible for this self-renewal and play a pivotal role in this skin homeostasis and in wound healing. Keratinocyte stem cells reside in a special microenvironment called niche in the basal layer of the epidermis, in the hair follicle bulge, and in sweat glands.

For this Special Issue on “Human Keratinocyte Stem Cells”, we encourage authors to contribute original research articles and comprehensive state-of-the-art reviews related to various aspects of human keratinocyte stem cell biology on cellular and molecular levels, including but not limited to such topics as stem cell markers, stem cell localization, stem cell characterization, stem cell potential, role of stem cells in health and disease, and use of stem cells for clinical applications.

Dr. Thomas Biedermann
Dr. Agnes Klar
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • keratinocyte stem cells
  • human skin
  • hair follicle stem cells
  • sweat gland stem cells
  • nail stem cells
  • integrins
  • cell adhesion molecules
  • genetic mutation
  • molecular biology
  • clinical translation
  • biomarker
  • skin tissue engineering
  • stem cell biology
  • intermediate filaments
  • keratin
  • gene mutation

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

13 pages, 4439 KiB  
Article
Investigating PEGDA and GelMA Microgel Models for Sustained 3D Heterotypic Dermal Papilla and Keratinocyte Co-Cultures
by Justin J.Y. Tan, Duc-Viet Nguyen, John E. Common, Chunyong Wu, Paul C.L. Ho and Lifeng Kang
Int. J. Mol. Sci. 2021, 22(4), 2143; https://doi.org/10.3390/ijms22042143 - 21 Feb 2021
Cited by 10 | Viewed by 3274
Abstract
Hair follicle morphogenesis is heavily dependent on reciprocal, sequential, and epithelial-mesenchymal interaction (EMI) between epidermal stem cells and the specialized cells of the underlying mesenchyme, which aggregate to form the dermal condensate (DC) and will later become the dermal papilla (DP). Similar models [...] Read more.
Hair follicle morphogenesis is heavily dependent on reciprocal, sequential, and epithelial-mesenchymal interaction (EMI) between epidermal stem cells and the specialized cells of the underlying mesenchyme, which aggregate to form the dermal condensate (DC) and will later become the dermal papilla (DP). Similar models were developed with a co-culture of keratinocytes and DP cells. Previous studies have demonstrated that co-culture with keratinocytes maintains the in vivo characteristics of the DP. However, it is often challenging to develop three-dimensional (3D) DP and keratinocyte co-culture models for long term in vitro studies, due to the poor intercellular adherence between keratinocytes. Keratinocytes exhibit exfoliative behavior, and the integrity of the DP and keratinocyte co-cultured spheroids cannot be maintained over prolonged culture. Short durations of culture are unable to sufficiently allow the differentiation and re-programming of the keratinocytes into hair follicular fate by the DP. In this study, we explored a microgel array approach fabricated with two different hydrogel systems. Using poly (ethylene glycol) diacrylate (PEGDA) and gelatin methacrylate (GelMA), we compare their effects on maintaining the integrity of the cultures and their expression of important genes responsible for hair follicle morphogenesis, namely Wnt10A, Wnt10B, and Shh, over prolonged duration. We discovered that low attachment surfaces such as PEGDA result in the exfoliation of keratinocytes and were not suitable for long-term culture. GelMA, on the hand, was able to sustain the integrity of co-cultures and showed higher expression of the morphogens overtime. Full article
(This article belongs to the Special Issue Human Keratinocytes Stem Cells)
Show Figures

Figure 1

Back to TopTop