Special Issue "Current Glycoproteomics: Theory, Methods and Applications"
A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".
Deadline for manuscript submissions: closed (31 December 2020) | Viewed by 21854
Interests: protein glycosylation; mitochondrial disease; proteoforms; proteomics; mass spectrometry; ion mobility
Special Issues, Collections and Topics in MDPI journals
Interests: hydrogen sulfide; metabolomics; mitochondrial biochemistry; neurodegenerative diseases; oxidative stress; proteomics; PTMs; redox dysregulation; sulfur species
Special Issues, Collections and Topics in MDPI journals
Protein glycosylation is a key mediator of protein biology and a major contributor to protein diversity in bacteria, archaea and eukaryota. Glycoprotein characterization by mass spectrometry has become a powerful tool to elucidate glycan structures on proteins and to investigate alterations in the glycosylation status of proteins in response to cellular changes. Aberrant protein glycosylation has been linked to various genetic and acquired human diseases which provides exciting opportunities for biomarker applications and rationale treatment strategies that modulate protein glycosylation. The glycosylation status of (poly)peptides are also important in industrial processes since protein glycosylation may impact a.o. production yields, functionality and stability of manufactured biologics (e.g. antibodies and glycoprotein conjugates), or trigger immunological responses following food intake or drug administration. Comprehensive analytical characterization of glycoproteins is therefore of great interest in (glyco)biology, healthcare and biotechnology.
Recent developments in mass-spectrometry-based glycoproteomics have enabled in-depth characterization of the glycan micro-heterogeneity (different glycan structures on a singly glycosylation site) and macro-heterogeneity (different combinations of glycan structures on a single protein molecule) for proteins in both simple and complex matrices such as biologics and blood plasma, respectively. Understanding the advantages and limitations of current mass spectrometry methods to characterize glycoproteins is essential to maximize the potential of glycoproteomics to support biological research, advance healthcare applications, and support industrial processes.
This special issue aims to provide essential background information and application examples to both glycoproteomics scientists and non-experts. We look for manuscripts to cover glycobiology, glycoproteomics technology and glycoproteomics applications in biological research, healthcare and industry. Both review and article manuscript types are welcomed that focus on any of the topics listed below. Please note that we will also accept manuscripts that make use of available (published) data to illustrate technological aspects and applications for educative purposes.
Main topics of this special issue include, but are not limited to:
- N- and O-glycosylation pathways and associated processes in human and other species (e.g. glycoprotein trafficking, glycoprotein export mechanisms, and sugar metabolism)
- Protein glycosylation in health and disease
- Glycoproteomics strategies: top-down/middle-down/bottom-up workflows*
- Sample preparation techniques
- Analytical separation technologies (e.g. liquid chromatography and capillary electrophoresis)
- ESI and MALDI of glycoproteins and glycopeptides
- Analysis of glycopeptides and glycoproteins by mass spectrometry
- Glycopeptide and glycoprotein characterization by complementary techniques (in relationship with mass spectrometry) such as ion mobility spectrometry or infrared ion spectroscopy
- Gas phase fragmentation techniques and mechanisms
- Interpretation of gas phase fragmentation data
- Computational approaches to (pre-)process and analyze glycoproteomics MSn data
- Bioinformatics to summarize, visualize, and interpret glycoproteomics results
- Methods to model analytical parameters of glyco(poly)peptides (e.g. chromatographic retention time, collisional cross section/ion mobility, ionization efficiency)
- Analysis of glycoproteins or glycoproteomes in health and disease
- Biomarker discovery
- Characterization/ QC of biologics (e.g. glycoprotein conjugates, antibodies and antibody drug conjugates)
* Note: Only approaches that use released glycan analysis to characterize purified glycoproteins will be accepted as this special issue specifically targets glycoprotein analysis in which glycan information is linked to its respective peptide- or protein-moiety.
Dr. Hans J.C.T. Wessels
Manuscript Submission Information
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