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New Advances in Glycobiotechnology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: closed (15 March 2024) | Viewed by 1222

Special Issue Editor


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Guest Editor
Laboratory of Advanced Chemical Biology, Hokkaido University, Sapporo 001-0021, Japan
Interests: organic chemistry; glycotechnology; glycomedicine; chemical biology; inhibitor; synthetic probe; mass spectrometry; microarray; infection; immunity; cancer; lectin; glycotyping

Special Issue Information

Dear Colleagues, 

Glycans (carbohydrates) are the fundamental substance in life, starting from photosynthesis. As a result, all living organisms (cells, tissues, etc.) are covered with glycocalyx, and these glycocalyces’ physical and biochemical functions have contributed to the adaptation (evolution) and diversity of organisms. In particular, biosynthetic pathways, sites of utilization, and forms of utilization are impossible to capture solely by deciphering the genes of single organisms. For this reason, the development of technologies to understand and utilize glycan functions has been vigorously conducted from various approaches. This Special Issue focuses on the development of glycobiotechnology, and calls for original research papers and review articles. Any technology and means (analytical, synthetic, or utilization methods) can be used as long as they are related to this subject.

We look forward to your active contributions.

Prof. Dr. Hiroshi Hinou
Guest Editor

Manuscript Submission Information

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Published Papers (1 paper)

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Research

10 pages, 4974 KiB  
Article
Sodium-Doped 3-Amino-4-hydroxybenzoic Acid: Rediscovered Matrix for Direct MALDI Glycotyping of O-Linked Glycopeptides and Intact Mucins
by Shogo Urakami and Hiroshi Hinou
Int. J. Mol. Sci. 2023, 24(23), 16836; https://doi.org/10.3390/ijms242316836 - 28 Nov 2023
Viewed by 867
Abstract
3-Amino-4-hydroxybenzoic acid (AHB) was the first matrix identified by glycoprotein glycan analysis using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). However, compared to commonly used matrices, such as 2,5-dihydroxybenzoic acid (DHB), AHB is less efficient at glycan ionization and lacks the ability to ionize [...] Read more.
3-Amino-4-hydroxybenzoic acid (AHB) was the first matrix identified by glycoprotein glycan analysis using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). However, compared to commonly used matrices, such as 2,5-dihydroxybenzoic acid (DHB), AHB is less efficient at glycan ionization and lacks the ability to ionize other molecular species, such as peptides, and thus is no longer used. In this study, we focused on the glycan-selective ionization ability of AHB and its low-noise properties in the low-molecular-weight region, as we expected that these properties could be enhanced by adding sodium to AHB. Sodium-doped AHB (AHB/Na) selectively imparts sodium adduct ions onto O-glycan fragments generated by the in-source decay (ISD) of glycopeptides and glycoproteins containing O-glycans that occurs during intense laser irradiation, enabling direct O-glycan analysis. Furthermore, we demonstrated that it is possible to investigate the internal structure of each O-glycan fragment with pseudo-MS/MS/MS using the sodium adduct ion of the O-glycan-derived ISD fragments from an intact mucin mixture. Full article
(This article belongs to the Special Issue New Advances in Glycobiotechnology)
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