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Biotechnology of Non-conventional Yeasts 2.0

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 1731

Special Issue Editor

Special Issue Information

Dear Colleagues,

In this Special Issue we encourage the submission of papers (research papers or reviews) on the biotechnology of non-conventional yeasts. Non-conventional yeasts have stimulated increasing interest with the development of molecular means that have made it possible to know the genomic profile of several yeast species with promising biotechnological application.

The present Special Issue is focused on recent developments in non-conventional yeasts biotechnology, with topics including recent techniques for characterizing yeasts and their physiology (including omics and nanobiotechnology techniques), methods to adapt industrial strains (including metabolic, synthetic, and evolutionary engineering), and the molecular aspects on the  use of yeasts as microbial cell factories to produce biopharmaceuticals, enzymes, alcohols, organic acids, flavors, and fine chemicals. There is a growing interest in the applications of non-conventional yeasts as starters for functional foods or fermented beverages, as well as producers of antimicrobial compounds. In this regard, further investigations on the molecular characteristics of these non-conventional yeasts needed.

Prof. Dr. Maurizio Ciani
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • non-conventional yeasts
  • yeast biotechnology
  • omics techniques
  • nanobiotechnology
  • industrial yeasts
  • cell factory
  • yeast metabolites
  • antimicrobial compounds
  • metabolic profile
  • yeast fermentation

Published Papers (1 paper)

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Research

14 pages, 3687 KiB  
Article
Comparative Zymocidial Effect of Three Different Killer Toxins against Brettanomyces bruxellensis Spoilage Yeasts
by Alice Agarbati, Maurizio Ciani, Semih Esin, Monica Agnolucci, Fabio Marcheggiani, Luca Tiano and Francesca Comitini
Int. J. Mol. Sci. 2023, 24(2), 1309; https://doi.org/10.3390/ijms24021309 - 09 Jan 2023
Cited by 1 | Viewed by 1249
Abstract
Three killer toxins that were previously investigated, one excreted by Kluyveromyces wickerhamii and two by different strains of Wickerhamomyces anomalus, were produced at the pilot scale, lyophilized and characterized, and the formulates were assessed for their zymocidial effect against Brettanomyces bruxellensis spoilage yeast. [...] Read more.
Three killer toxins that were previously investigated, one excreted by Kluyveromyces wickerhamii and two by different strains of Wickerhamomyces anomalus, were produced at the pilot scale, lyophilized and characterized, and the formulates were assessed for their zymocidial effect against Brettanomyces bruxellensis spoilage yeast. A comparative analysis allowed the evaluation of the minimum inhibitory concentration (MIC) against a sensitive strain. Fungicidal and fungistatic concentrations were used to evaluate the cytocidal effect using a cytofluorimetric approach that confirmed the lethal effect of all lyophilized formulates against B. bruxellensis spoilage yeasts. Moreover, the potential killer toxins’ cytotoxicity against human intestinal cells (Caco-2) were evaluated to exclude any possible negative effect on the consumers. Finally, the effective lethal effect of all three lyophilized killer toxins toward B. bruxellensis sensitive strain were tested. The results indicated that all of them acted without dangerous effects on the human epithelial cells, opening the way for their possible commercial application. In particular, D15 showed the lowest MIC and the highest activity, was evaluated also in wine, revealing a strong reduction of Brettamonyces yeast growth and, at the same time, a control of ethyl phenols production. Full article
(This article belongs to the Special Issue Biotechnology of Non-conventional Yeasts 2.0)
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