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Advances in Molecular and Cellular Imaging, Microscopy, and Biomedical Spectroscopy

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 1582

Special Issue Editor

Faculty of Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan
Interests: fluorescent imaging; raman spectroscopy; nonlinear microscopy; optical biopsy; cancer metastasis; cancer diagnosis; osteoporosis; cartilage degeneration and regeneration
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Optical imaging and spectroscopic techniques are promising tools used to visualize molecular dynamics in living cells, organoids, and tissues in the fields of developmental biology, tissue engineering, immune response, tumorigenesis, and regenerative medicine. Recent advances in laser optics, imaging and microscopic technologies, and molecular probes have drastically improved sensitivity, specificity, time and spatial resolution, penetration dept, etc., for molecular and cellular imaging, microscopy, and biomedical spectroscopy. This Special Issue encourages the publication of methodologies that help to elucidate molecular mechanisms, cellular functions, and tissue morphologies in biological systems with and/or without labeling. Various research themes and topics (including nonlinear optical imaging; multiphoton fluorescence; SHG, THG, CARS, and SRS microscopy; photoacoustic imaging; NIR imaging and spectroscopy; and spontaneous Raman spectroscopy) are very welcome, in combination with modality and molecular sciences.

Dr. Yusuke Oshima
Guest Editor

Manuscript Submission Information

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Published Papers (2 papers)

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Research

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14 pages, 1613 KiB  
Article
Establishing Monoclonal Gammopathy of Undetermined Significance as an Independent Pre-Disease State of Multiple Myeloma Using Raman Spectroscopy, Dynamical Network Biomarker Theory, and Energy Landscape Analysis
Int. J. Mol. Sci. 2024, 25(3), 1570; https://doi.org/10.3390/ijms25031570 - 26 Jan 2024
Viewed by 547
Abstract
Multiple myeloma (MM) is a cancer of plasma cells. Normal (NL) cells are considered to pass through a precancerous state, such as monoclonal gammopathy of undetermined significance (MGUS), before transitioning to MM. In the present study, we acquired Raman spectra at three stages—834 [...] Read more.
Multiple myeloma (MM) is a cancer of plasma cells. Normal (NL) cells are considered to pass through a precancerous state, such as monoclonal gammopathy of undetermined significance (MGUS), before transitioning to MM. In the present study, we acquired Raman spectra at three stages—834 NL, 711 MGUS, and 970 MM spectra—and applied the dynamical network biomarker (DNB) theory to these spectra. The DNB analysis identified MGUS as the unstable pre-disease state of MM and extracted Raman shifts at 1149 and 1527–1530 cm1 as DNB variables. The distribution of DNB scores for each patient showed a significant difference between the mean values for MGUS and MM patients. Furthermore, an energy landscape (EL) analysis showed that the NL and MM stages were likely to become stable states. Raman spectroscopy, the DNB theory, and, complementarily, the EL analysis will be applicable to the identification of the pre-disease state in clinical samples. Full article
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19 pages, 2993 KiB  
Review
Pushing the Resolution Limit of Stimulated Emission Depletion Optical Nanoscopy
Int. J. Mol. Sci. 2024, 25(1), 26; https://doi.org/10.3390/ijms25010026 - 19 Dec 2023
Viewed by 705
Abstract
Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to [...] Read more.
Optical nanoscopy, also known as super-resolution optical microscopy, has provided scientists with the means to surpass the diffraction limit of light microscopy and attain new insights into nanoscopic structures and processes that were previously inaccessible. In recent decades, numerous studies have endeavored to enhance super-resolution microscopy in terms of its spatial (lateral) resolution, axial resolution, and temporal resolution. In this review, we discuss recent efforts to push the resolution limit of stimulated emission depletion (STED) optical nanoscopy across multiple dimensions, including lateral resolution, axial resolution, temporal resolution, and labeling precision. We introduce promising techniques and methodologies building on the STED concept that have emerged in the field, such as MINSTED, isotropic STED, and event-triggered STED, and evaluate their respective strengths and limitations. Moreover, we discuss trade-off relationships that exist in far-field optical microscopy and how they come about in STED optical nanoscopy. By examining the latest developments addressing these aspects, we aim to provide an updated overview of the current state of STED nanoscopy and its potential for future research. Full article
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