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Fluorescent Materials and Biological Subcellular Localization

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 4962

Special Issue Editors


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Guest Editor
1. Jiangxi Key Laboratory for Microscale Interdisciplinary Study, Institute for Advanced Study, Nanchang University, Nanchang 330031, China
2. College of Life Sciences, Nanchang University, Nanchang 330031, China
Interests: lipoproteins, gangliosides, and atherosclerosis; drug delivery systems; membrane vesicles; pharmacology of cyclodextrins; fluorescent probes/sensors
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Guest Editor
Department of Chemistry, Nanchang University, Nanchang 330031, China
Interests: synthesis of novel fluorescent materials; fluorescent nanoparticles for biomedical applications; host-guest chemistry; supra-molecular self assembly; fluorescent sensor

Special Issue Information

Dear Colleagues,

Subcellular structures or organelles, including the nucleus, mitochondria, lysosomes, endoplasmic reticulum (ER), Golgi apparatus, lipid droplets, lipid rafts, and so on, play important roles in ensuring the smooth progress of cell growth, proliferation, division, signal transduction, apoptosis, and metabolism, among others. The local properties of the microenvironments surrounding or within subcellular structures/organelles, including pH value, temperature, viscosity, electrostatic property, concentrations of specific small biomolecules, etc., can significantly influence the functions of subcellular structures. Studying the ultra-fine structure and functions of subcellular structures, as well as the biophysical or physicochemical properties of microenvironments, at a subcellular level has garnered tremendous attention in recent years. This attention has created a great demand for fluorescent materials localizing subcellular structures/organelles and sensing the changes in various properties of their microenvironments.

The application of fluorescent materials in biological system is rapidly growing. Different types of fluorescent materials, including organic small molecules, coordination compounds, polymers, inorganic nanoparticles (semiconductor quantum dots, carbon dots, MOF materials, nanoclusters), and hybrid nanoparticles have been used for bioimaging, monitoring biological species, and photodynamic therapy. However, these fluorescent materials were generally used to target specific tissues or cells. In recent years, fluorescent materials able to target subcellular structures/organelles or detect local properties of microenvironments have been rapidly developing. This Special Issue of the International Journal of Molecular Sciences aims to collect papers about the advances in fluorescent materials (fluorescent dyes or sensors) for subcellular localization or for detecting biophysical or physicochemical properties of subcellular structures/organelles or microenvironments.

Prof. Dr. Yong Chen
Prof. Dr. Qianyong Cao
Guest Editors

Manuscript Submission Information

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Keywords

  • fluorescent materials
  • fluorescent dyes
  • subcellular localization
  • MOF materials
  • nanoclusters
  • semiconductor quantum dots
  • carbon dots

Published Papers (2 papers)

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Research

13 pages, 2268 KiB  
Article
Meta-CF3-Substituted Analogues of the GFP Chromophore with Remarkable Solvatochromism
by Maxim M. Perfilov, Elvira R. Zaitseva, Nadezhda S. Baleeva, Vadim S. Kublitski, Alexander Yu. Smirnov, Yulia A. Bogdanova, Svetlana A. Krasnova, Ivan N. Myasnyanko, Alexander S. Mishin and Mikhail S. Baranov
Int. J. Mol. Sci. 2023, 24(12), 9923; https://doi.org/10.3390/ijms24129923 - 08 Jun 2023
Cited by 2 | Viewed by 1152
Abstract
In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a pronounced solvent-dependent gradation of [...] Read more.
In this work, we have shown that the introduction of a trifluoromethyl group into the me-ta-position of arylidene imidazolones (GFP chromophore core) leads to a dramatic increase in their fluorescence in nonpolar and aprotic media. The presence of a pronounced solvent-dependent gradation of fluorescence intensity makes it possible to use these substances as fluorescent polarity sensors. In particular, we showed that one of the created compounds could be used for selective labeling of the endoplasmic reticulum of living cells. Full article
(This article belongs to the Special Issue Fluorescent Materials and Biological Subcellular Localization)
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16 pages, 11690 KiB  
Article
Simple and Highly Efficient Detection of PSD95 Using a Nanobody and Its Recombinant Heavy-Chain Antibody Derivatives
by Markus Kilisch, Maja Gere-Becker, Liane Wüstefeld, Christel Bonnas, Alexander Crauel, Maja Mechmershausen, Henrik Martens, Hansjörg Götzke, Felipe Opazo and Steffen Frey
Int. J. Mol. Sci. 2023, 24(8), 7294; https://doi.org/10.3390/ijms24087294 - 14 Apr 2023
Cited by 2 | Viewed by 2648
Abstract
The post-synaptic density protein 95 (PSD95) is a crucial scaffolding protein participating in the organization and regulation of synapses. PSD95 interacts with numerous molecules, including neurotransmitter receptors and ion channels. The functional dysregulation of PSD95 as well as its abundance and localization has [...] Read more.
The post-synaptic density protein 95 (PSD95) is a crucial scaffolding protein participating in the organization and regulation of synapses. PSD95 interacts with numerous molecules, including neurotransmitter receptors and ion channels. The functional dysregulation of PSD95 as well as its abundance and localization has been implicated with several neurological disorders, making it an attractive target for developing strategies able to monitor PSD95 accurately for diagnostics and therapeutics. This study characterizes a novel camelid single-domain antibody (nanobody) that binds strongly and with high specificity to rat, mouse, and human PSD95. This nanobody allows for more precise detection and quantification of PSD95 in various biological samples. We expect that the flexibility and unique performance of this thoroughly characterized affinity tool will help to further understand the role of PSD95 in normal and diseased neuronal synapses. Full article
(This article belongs to the Special Issue Fluorescent Materials and Biological Subcellular Localization)
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