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Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, China
Interests: medicinal plants; plant breeding; genetic improvement for new cultivar development; plant autophagy

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Dear Colleagues,

Scientists have long sought to understand gene function in crops by mining natural plant mutants or using random mutagenesis as a tool, which is costly and inefficient. The emergence of gene editing technology has significantly enhanced the work of plant gene function research, such as TALEN, CRISPR, etc. CRISPR is undoubtedly the most powerful tool of these genome-editing technologies. It is currently the simplest and most versatile and precise method of genetic manipulation and is therefore generating a buzz in the plant research world. CRISPR technology can not only be used to study gene function but is also widely used in the directional breeding of plants, realizing the rapid domestication of crops.

Given this context, in this Special Issue, articles (original research papers, perspectives, hypotheses, opinions, reviews, modeling approaches, and methods) that focus on gene discovery, molecular methods, and molecular breeding of plant biology using CRISPR genome-editing technology are most welcome.

Dr. Weiming Hu
Guest Editor

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Keywords

genome editing
CRISPR
plant
crop
molecular breeding
gene discovery
CRISPR-mediated plant base editors

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Research

Open AccessArticle

CRISPR/Cas9-Mediated Mutagenesis of BrLEAFY Delays the Bolting Time in Chinese Cabbage (Brassica rapa L. ssp. pekinensis)

Yun-Hee Shin

and

Young-Doo Park

Int. J. Mol. Sci. 2023, 24(1), 541; https://doi.org/10.3390/ijms24010541 - 29 Dec 2022

Cited by 1 | Viewed by 1037

Abstract

Chinese cabbage has unintended bolting in early spring due to sudden climate change. In this study, late-bolting Chinese cabbage lines were developed via mutagenesis of the BrLEAFY (BrLFY) gene, a transcription factor that determines floral identity, using the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system. Double-strand break of the target region via gene editing based on nonhomologous end joining (NHEJ) was applied to acquire useful traits in plants. Based on the ‘CT001’ pseudomolecule, a single guide RNA (sgRNA) was designed and the gene-editing vector was constructed. Agrobacterium-mediated transformation was used to generate a Chinese cabbage line in which the sequence of the BrLFY paralogs was edited. In particular, single base inserted mutations occurred in the BrLFY paralogs of the LFY-7 and LFY-13 lines, and one copy of T-DNA was inserted into the intergenic region. The selected LFY-edited lines displayed continuous vegetative growth and late bolting compared to the control inbred line, ‘CT001’. Further, some LFY-edited lines showing late bolting were advanced to the next generation. The T-DNA-free E₁LFY-edited lines bolted later than the inbred line, ‘CT001’. Overall, CRISPR/Cas9-mediated mutagenesis of the BrLFY gene was found to delay the bolting time. Accordingly, CRISPR/Cas9 is considered an available method for the molecular breeding of crops. Full article

(This article belongs to the Special Issue CRISPR Base Editor for Molecular Plant Sciences)

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