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Current Insights into Nucleic Acids

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: 25 July 2024 | Viewed by 500

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Guest Editor
1. Department of Medicine and Immunology, Division of Rheumatology and Immunology, Duke University Medical Center, Durham, NC 27710, USA
2. Medical Research Service, Veterans Administration Medical Center, Durham, NC 27705, USA
Interests: immune properties of DNA; immunochemical properties of anti-DNA antibodies; the properties of antinuclear antibodies; the role of DNA in the pathogenesis of systemic lupus erythematosus; the role of microparticles as a source of extracellular DNA; immune properties of HMGB1
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Special Issue Information

Dear Colleagues,

Nucleic acids are a diverse group of macromolecules that are essential for all life. While the basic principles of heredity and differentiated cell function have long been established, recent studies have revealed exciting insights into the various roles of DNA and RNA, both intracellular and extracellular, in biology, genomic structure, regulatory RNA, and the immune activities of nucleic acids. This Special Issue of IJMS will focus on the structural and functional roles of DNA and RNA, including advances in delineating higher-order interactions in gene function. The role of different enzymes in DNA metabolism will also be considered. Genetic studies are of interest if they illustrate important aspects of genomic structure or regulation. Papers for this Special Issue can encompass studies of both eukaryotic and prokaryotic systems as well as viruses and other infecting organisms. This Special Issue will also consider papers on methodologies including novel approaches for gene sequencing and structural analysis.

Prof. Dr. David Stephen Pisetsky
Guest Editor

Manuscript Submission Information

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Keywords

  • nucleic acids
  • DNA
  • RNA
  • genome
  • gene function
  • gene regulation
  • gene structure

Published Papers (1 paper)

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Research

19 pages, 3934 KiB  
Article
The ArgR-Regulated ADI Pathway Facilitates the Survival of Vibrio fluvialis under Acidic Conditions
by Qian Cheng, Yu Han, Yue Xiao, Zhe Li, Aiping Qin, Saisen Ji, Biao Kan and Weili Liang
Int. J. Mol. Sci. 2024, 25(11), 5679; https://doi.org/10.3390/ijms25115679 - 23 May 2024
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Abstract
Vibrio fluvialis is an emerging foodborne pathogenic bacterium that can cause severe cholera-like diarrhea and various extraintestinal infections, posing challenges to public health and food safety worldwide. The arginine deiminase (ADI) pathway plays an important role in bacterial environmental adaptation and pathogenicity. However, [...] Read more.
Vibrio fluvialis is an emerging foodborne pathogenic bacterium that can cause severe cholera-like diarrhea and various extraintestinal infections, posing challenges to public health and food safety worldwide. The arginine deiminase (ADI) pathway plays an important role in bacterial environmental adaptation and pathogenicity. However, the biological functions and regulatory mechanisms of the pathway in V. fluvialis remain unclear. In this study, we demonstrate that L-arginine upregulates the expression of the ADI gene cluster and promotes the growth of V. fluvialis. The ADI gene cluster, which we proved to be comprised of two operons, arcD and arcACB, significantly enhances the survival of V. fluvialis in acidic environments both in vitro (in culture medium and in macrophage) and in vivo (in mice). The mRNA level and reporter gene fusion analyses revealed that ArgR, a transcriptional factor, is necessary for the activation of both arcD and arcACB transcriptions. Bioinformatic analysis predicted the existence of multiple potential ArgR binding sites at the arcD and arcACB promoter regions that were further confirmed by electrophoretic mobility shift assay, DNase I footprinting, or point mutation analyses. Together, our study provides insights into the important role of the ArgR-ADI pathway in the survival of V. fluvialis under acidic conditions and the detailed molecular mechanism. These findings will deepen our understanding of how environmental changes and gene expression interact to facilitate bacterial adaptations and virulence. Full article
(This article belongs to the Special Issue Current Insights into Nucleic Acids)
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