Special Issue "The Mechanism and Emerging Materials in Thiamine Catalysis"
Deadline for manuscript submissions: 15 October 2023 | Viewed by 818
Transketolase (TK) is a kind of enzyme that is encoded by the TKT gene. As a soluble enzyme, it occurs in the cellular and extra-cellular fluid of animals, plants, and microorganisms. In animals it is present in high amounts in adipose tissue, mammary glands, the adrenal cortex, and erythrocytes. The TK level in serum is of diagnostic interest for liver diseases and cancer, while changes in the activity of this enzyme are of interest for erythrocytes and tissues with thiamine deficiency. Its cofactor, thiamine diphosphate (ThDP), is required for processes of general metabolism across all organisms. TK is a homodimeric enzyme with the kinetic properties of half-of-the-sites reactivity, and negative cooperativity. These structural and kinetic features arise as a result of the reciprocal coupling of active sites located in the area of intersubunit contact.
Transketolase is investigated by structural and kinetic methods, as well as in modeling systems. Compared with the two-substrate reaction mode, its lower rate makes it possible to obtain reaction intermediates. The nature of the nonequivalence of transketolase active sites during keto substrate binding was also clarified, which most likely consists of switching the simultaneous binding and cleavage of substrates to their alternate binding (flip-flop mechanism). Furthermore, RNA seasonally and non-covalently bound to transketolase has been shown to be a ribozyme with triose phosphate isomerase activity.
This Special Issue welcomes all the research about mechanisms and materials used in transketolase catalysis.
Dr. Olga N. Solovjeva
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- thiamine diphosphate-dependent enzymes
- thiamine catalysis
- mass spectrometry
- conformational mobility of enzyme molecule