ijms-logo

Journal Browser

Journal Browser

Application of Fluorescence Imaging and Super-Resolution Imaging in Molecular Biology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biophysics".

Deadline for manuscript submissions: 16 September 2024 | Viewed by 1290

Special Issue Editor


E-Mail Website
Guest Editor
KMU Biobank Center, Institute of Biomedical Science, Kansai Medical University, 573-1010 Hirakata, Japan
Interests: super-resolution imaging; molecular biology; neuroscience; hippocampus; new CA2 region; new technologies; BDNF

Special Issue Information

Dear Colleagues,

Recent inventions of super-resolution microscopies have induced a revolution in molecular biology and enabled us to see various biological molecules, organelles, cells, and disease events at the nanoscale. In addition, evolutions of fluorescence imaging technologies have also contributed to progress in various areas of molecular biology. International Journal of Molecular Sciences will publish a Special Issue in 2023 to highlight studies in molecular biology that use fluorescence imaging and super-resolution microscopy.

We therefore invite reviews and research articles on the application of fluorescence imaging in molecular biology and super-resolution imaging in molecular biology.

We will collect a series of articles that include fluorescence imaging and super-resolution imaging in molecular biology, molecular disease biology, and methodologies of fluorescence and super-resolution imaging.

The related topics include, but are not limited to:

  • Fluorescence imaging of biomolecules or organelles;
  • Fluorescence imaging of the morphologies of cells;
  • Fluorescence imaging in iPS cells or organoids;
  • Fluorescence imaging in animal disease models;
  • Methodologies of fluorescence imaging for molecular biology;
  • Super-resolution imaging of biomolecules or organelles;
  • Super-resolution imaging of the morphologies of cells;
  • Super-resolution imaging in animal disease models;
  • Super-resolution imaging in iPS cells or organoids;
  • Methodologies of super-resolution microscopies for molecular biology.

Dr. Keigo Kohara
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescence imaging
  • super-resolution imaging
  • molecular biology
  • disease
  • biomolecules
  • organelles
  • morphologies of cells
  • iPS cells
  • organoids
  • molecular biology

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

21 pages, 20175 KiB  
Article
From Cell Populations to Molecular Complexes: Multiplexed Multimodal Microscopy to Explore p53-53BP1 Molecular Interaction
by Simone Pelicci, Laura Furia, Pier Giuseppe Pelicci and Mario Faretta
Int. J. Mol. Sci. 2024, 25(9), 4672; https://doi.org/10.3390/ijms25094672 - 25 Apr 2024
Viewed by 608
Abstract
Surpassing the diffraction barrier revolutionized modern fluorescence microscopy. However, intrinsic limitations in statistical sampling, the number of simultaneously analyzable channels, hardware requirements, and sample preparation procedures still represent an obstacle to its widespread diffusion in applicative biomedical research. Here, we present a novel [...] Read more.
Surpassing the diffraction barrier revolutionized modern fluorescence microscopy. However, intrinsic limitations in statistical sampling, the number of simultaneously analyzable channels, hardware requirements, and sample preparation procedures still represent an obstacle to its widespread diffusion in applicative biomedical research. Here, we present a novel pipeline based on automated multimodal microscopy and super-resolution techniques employing easily available materials and instruments and completed with open-source image-analysis software developed in our laboratory. The results show the potential impact of single-molecule localization microscopy (SMLM) on the study of biomolecules’ interactions and the localization of macromolecular complexes. As a demonstrative application, we explored the basis of p53-53BP1 interactions, showing the formation of a putative macromolecular complex between the two proteins and the basal transcription machinery in situ, thus providing visual proof of the direct role of 53BP1 in sustaining p53 transactivation function. Moreover, high-content SMLM provided evidence of the presence of a 53BP1 complex on the cell cytoskeleton and in the mitochondrial space, thus suggesting the existence of novel alternative 53BP1 functions to support p53 activity. Full article
Show Figures

Figure 1

Back to TopTop