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New Insights into Gene Expression Regulation in the Next-Generation Sequencing (NGS) Era

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 130

Special Issue Editor

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Guest Editor
Department of Pathology, Faculty of Medicine, University of Thessaly, 41500 Larissa, Greece
Interests: urothelial carcinomas genetics and genomics; epigenetics; cancer biology; molecular subtypes; mutations; signaling pathways; miRNAs

Special Issue Information

Dear Colleagues,

Somatic cells exhibit different phenotypes, though they share the same DNA sequences. Gene expression regulation is achieved through transcriptional, post-transcriptional, translational, and post-translational mechanisms. Epigenetic factors modulate gene expression through DNA methylation, histone protein modifications, and RNA-molecule interaction processes. Next-generation sequencing (NGS) is a high-throughput technique that has boosted gene regulation studies. RNA sequencing (RNA-seq) allows transcriptome analysis and provides evidence about gene regulation, alternative splicing, and non-coding RNA molecules. Tissue heterogeneity and the microenvironment also add to the complexity. NGS-based approaches, such as single-cell or spatial RNA sequencing, can evaluate the mRNA expressions of all cells included in a specific microenvironment, providing evidence about cell–cell interactions. Methyl-seq or CHIP-seq NGS approaches will permit epigenome understanding through studies on DNA methylation patterns and histone modifications. The human microbiome is also a key player in gene expression regulation. Thus, the use of the NGS technique in metagenomic studies will highlight the role and mechanisms of microbiome interaction with human host cells.

I propose that the scope of this Special Issue of IJMS include a wide range of studies that use NGS-based approaches to investigate various aspects of gene expression regulation. Submission formats may include research articles, reviews, perspectives, and communications.

Dr. Maria Samara
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • alternative splicing
  • DNA methylation
  • histone modification
  • RNA sequencing
  • epigenome
  • metagenomics
  • single-cell sequencing
  • spatial sequencing
  • microenvironment
  • microbiome profile

Published Papers

This special issue is now open for submission.
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