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Special Issue "Plant Genetic and Epigenetic Engineering: New Advances and Molecular Tools"

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Dear Colleagues,

Plant biotechnology is considered a collection of breakthrough technologies that can help meet challenges in agriculture, horticulture, and forestry to obain plants with improved production properties and resilience to environmental cues. Plant biotechnology is an intensively developing field aimed at modifying plant properties with the assistance of modern molecular and physiolgical techniques in order to reach new or improved desirable plant characteristics. This Special Issue welcomes original research papers, reviews, communications, and opinion papers presenting new advances in the field of plant biotechnology and genetic engineering.

We welcome papers including, but not limited to, the following specific topics:

New advances in plant genetic engineering;
New advances using CRISPR–Cas technology;
Plant cell culture-based biotechnological approaches;
Creation, conservation, and utilization of genetic crop variability;
RNAi-based plant gene regulation techniques;
Host-induced gene silencing (HIGS);
Virus-induced gene silencing (VIGS);
Spray-induced gene silencing (SIGS);
Plant endophytes and their biotechnological applications;
Modern breeding methods;
Social, environmental, and global aspects of plant biotechnology.

Dr. Konstantin V. Kiselev
Dr. Alexandra S. Dubrovina
Guest Editors

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Keywords

plant biotechnolgoy
genetic engineering
plant cell cultures
transgenic plants
CRISPR–Cas
plant gene regulation
RNA interference
gene silencing
crop variability
stress resistance

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Research

Open AccessArticle

Impact of Intron and Retransformation on Transgene Expression in Leaf and Fruit Tissues of Field-Grown Pear Trees

Vadim Lebedev

Int. J. Mol. Sci. 2023, 24(16), 12883; https://doi.org/10.3390/ijms241612883 - 17 Aug 2023

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Abstract

Stable and high expression of introduced genes is a prerequisite for using transgenic trees. Transgene stacking enables combining several valuable traits, but repeated transformation increases the risk of unintended effects. This work studied the stability and intron-mediated enhancement of uidA gene expression in leaves and different anatomical parts of pear fruits during field trials over 14 years. The stability of reporter and herbicide resistance transgenes in retransformed pear plants, as well as possible unintended effects using high-throughput phenotyping tools, were also investigated. The activity of β-glucuronidase (GUS) varied depending on the year, but silencing did not occur. The uidA gene was expressed to a maximum in seeds, slightly less in the peel and peduncles, and much less in the pulp of pear fruits. The intron in the uidA gene stably increased expression in leaves and fruits by approximately twofold. Retransformants with the bar gene showed long-term herbicide resistance and exhibited no consistent changes in leaf size and shape. The transgenic pear was used as rootstock and scion, but grafted plants showed no transport of the GUS protein through the graft in the greenhouse and field. This longest field trial of transgenic fruit trees demonstrates stable expression under varying environmental conditions, the expression-enhancing effect of intron and the absence of unintended effects in single- and double-transformed woody plants. Full article

(This article belongs to the Special Issue Plant Genetic and Epigenetic Engineering: New Advances and Molecular Tools)

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Special Issue "Plant Genetic and Epigenetic Engineering: New Advances and Molecular Tools"

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