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Molecular Crop Management: Emerging Role of Nucleic Acids in Agriculture
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Molecular Crop Management: Emerging Role of Nucleic Acids in Agriculture

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 6807

Special Issue Editor

Dr. Volodymyr V. Oberemok

E-Mail Website
Guest Editor
Department of Molecular Genetics and Biotechnologies, V.I. Vernadsky Crimean Federal University, 295007 Simferopol, Crimea
Interests: oligonucleotide insecticides; DNA insecticides; antisense oligoilators; DNA synthesis; plant protection; green agriculture
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Antisense DNA and RNA oligonucleotides, microRNAs, siRNA, and long double-stranded RNA are polymers based on DNA or RNA chemistries and synthesized in vitro, which regulate gene expression by binding in a sequence-specific manner to an RNA target. When considering the variety of successful oligonucleotide modifications created for medicine with an efficient signal-to-noise ratio of action, coupled with the affordability of in vitro oligonucleotide synthesis and post-synthesis procedures, we predict that in the near future, a fruitful yield of tools for green agriculture will also be produced, created from effective nucleic acid-based end-products. The regulatory role of DNA and RNA goes beyond the cell nucleus, cytoplasm, and cell itself and can be an effective tool for molecular crop yield management. Additionally, climate change is driving the need in agriculture for safe, effective, affordable preparations with a low carbon footprint, such as nucleic-acid-based products.

This Special Issue aims to discuss the role of nucleic acids as perspective preparations for green agriculture. In particular, topics such as contact oligonucleotide insecticides (DNA insecticides) and the RNAi approach for insect pest control and oligoilators for management of accumulation of secondary metabolites in essential oil plants will be addressed in this issue. This issue aims to provide an in-depth picture of future green agriculture provided by end-products based on nucleic acids.

Dr. Volodymyr V. Oberemok
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • DNA
  • RNA
  • agriculture
  • nucleic acids
  • siRNA
  • microRNA
  • double-stranded RNA
  • RNAi
  • anti-sense oligonucleotides
  • oligonucleotide insecticides
  • DNA insecticides
  • oligoilators

Published Papers (3 papers)

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Research

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12 pages, 837 KiB  
Article
Icerya purchasi Maskell (Hemiptera: Monophlebidae) Control Using Low Carbon Footprint Oligonucleotide Insecticides
by Nikita V. Gal’chinsky, Ekaterina V. Yatskova, Ilya A. Novikov, Refat Z. Useinov, Nanan J. Kouakou, Kra F. Kouame, Kouadio D. Kra, Alexander K. Sharmagiy, Yuri V. Plugatar, Kateryna V. Laikova and Volodymyr V. Oberemok
Int. J. Mol. Sci. 2023, 24(14), 11650; https://doi.org/10.3390/ijms241411650 - 19 Jul 2023
Cited by 2 | Viewed by 1104
Abstract
Climate change creates favourable conditions for the growth of insect populations. Today, the world is seeing an increase in the number of insect pest infestations associated with a long-term increase in the average temperature of climatic systems. For example, local invasions of Icerya [...] Read more.
Climate change creates favourable conditions for the growth of insect populations. Today, the world is seeing an increase in the number of insect pest infestations associated with a long-term increase in the average temperature of climatic systems. For example, local invasions of Icerya purchasi Maskell, a citrus pest recognized worldwide, have increased in size and number in recent years. Controlling this pest is complicated because not all chemical insecticides are effective, and their use is undesirable since citrus fruit is used for food and chemical agents cumulatively harm human health. In this article, we demonstrated for the first time the successful use of a short single-stranded fragment of the 28S ribosomal RNA gene called “oligoICER-11” to control cottony cushion scale, and we propose the use of green oligonucleotide insecticides with a low carbon footprint for large-scale implementation in agriculture and forestry. Using the contact oligonucleotide insecticide oligoICER-11 at a concentration of 100 ng/μL on I. purchasi larvae resulted in a mortality of 70.55 ± 0.77% within 10 days. Thus, climate change is driving the need in both agriculture and forestry for oligonucleotide insecticides (DNA insecticides, olinscides): safe, effective, affordable insecticides with a low carbon footprint and long operational life. Full article
(This article belongs to the Special Issue Molecular Crop Management: Emerging Role of Nucleic Acids in Agriculture)
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11 pages, 2507 KiB  
Article
Oligonucleotide Insecticides for Green Agriculture: Regulatory Role of Contact DNA in Plant–Insect Interactions
by Volodymyr V. Oberemok, Refat Z. Useinov, Oleksii A. Skorokhod, Nikita V. Gal’chinsky, Ilya A. Novikov, Tatyana P. Makalish, Ekaterina V. Yatskova, Alexander K. Sharmagiy, Ilya O. Golovkin, Yuri I. Gninenko, Yelizaveta V. Puzanova, Oksana A. Andreeva, Edie E. Alieva, Emre Eken, Kateryna V. Laikova and Yuri V. Plugatar
Int. J. Mol. Sci. 2022, 23(24), 15681; https://doi.org/10.3390/ijms232415681 - 10 Dec 2022
Cited by 4 | Viewed by 2069
Abstract
Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need [...] Read more.
Insects vastly outnumber us in terms of species and total biomass, and are among the most efficient and voracious consumers of plants on the planet. As a result, to preserve crops, one of the primary tasks in agriculture has always been the need to control and reduce the number of insect pests. The current use of chemical insecticides leads to the accumulation of xenobiotics in ecosystems and a decreased number of species in those ecosystems, including insects. Sustainable development of human society is impossible without useful insects, so the control of insect pests must be effective and selective at the same time. In this article, we show for the first time a natural way to regulate the number of insect pests based on the use of extracellular double-stranded DNA secreted by the plant Pittosporum tobira. Using a principle similar to one found in nature, we show that the topical application of artificially synthesized short antisense oligonucleotide insecticides (olinscides, DNA insecticides) is an effective and selective way to control the insect Coccus hesperidum. Using contact oligonucleotide insecticide Coccus-11 at a concentration of 100 ng/μL on C. hesperidum larvae resulted in a mortality of 95.59 ± 1.63% within 12 days. Green oligonucleotide insecticides, created by nature and later discovered by humans, demonstrate a new method to control insect pests that is beneficial and safe for macromolecular insect pest management. Full article
(This article belongs to the Special Issue Molecular Crop Management: Emerging Role of Nucleic Acids in Agriculture)
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Review

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18 pages, 663 KiB  
Review
Insights into the Effects of Insecticides on Aphids (Hemiptera: Aphididae): Resistance Mechanisms and Molecular Basis
by Rana Muhammad Kaleem Ullah, Fukun Gao, Aatika Sikandar and Haiyan Wu
Int. J. Mol. Sci. 2023, 24(7), 6750; https://doi.org/10.3390/ijms24076750 - 04 Apr 2023
Cited by 4 | Viewed by 2834
Abstract
With the passage of time and indiscreet usage of insecticides on crops, aphids are becoming resistant to their effect. The different classes of insecticides, including organophosphates, carbamates, pyrethroids and neonicotinoids, have varied effects on insects. Furthermore, the molecular effects of these insecticides in [...] Read more.
With the passage of time and indiscreet usage of insecticides on crops, aphids are becoming resistant to their effect. The different classes of insecticides, including organophosphates, carbamates, pyrethroids and neonicotinoids, have varied effects on insects. Furthermore, the molecular effects of these insecticides in aphids, including effects on the enzymatic machinery and gene mutation, are resulting in aphid resistance to the insecticides. In this review, we will discuss how aphids are affected by the overuse of pesticides, how resistance appears, and which mechanisms participate in the resistance mechanisms in various aphid species as significant crop pests. Gene expression studies were analyzed using the RNA-Seq technique. The stress-responsive genes were analyzed, and their expression in response to insecticide administration was determined. Putative insecticide resistance-related genes, cytochrome P450, glutathione S-transferase, carboxylesterase CarEs, ABC transporters, cuticle protein genes, and trypsin-related genes were studied. The review concluded that if insecticide-susceptible aphids interact with ample dosages of insecticides with sublethal effects, this will result in the upregulation of genes whose primary role is to detoxify insecticides. In the past decade, certain advancements have been observed regarding insecticide resistance on a molecular basis. Even so, not much is known about how aphids detoxify the insecticides at molecular level. Thus, to attain equilibrium, it is important to observe the manipulation of pest and insect species with the aim of restoring susceptibility to insecticides. For this purpose, this review has included critical insights into insecticide resistance in aphids. Full article
(This article belongs to the Special Issue Molecular Crop Management: Emerging Role of Nucleic Acids in Agriculture)
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