Selected Papers from the 3rd International Conference “Genetically Modified Organisms: The Нistory, Achievements, Social and Environmental Risks”

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Genetics, Genomics, Breeding, and Biotechnology (G2B2)".

Deadline for manuscript submissions: closed (1 December 2023) | Viewed by 4399

Special Issue Editor


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Guest Editor
Department of Genetics and Biotechnology, Saint Petersburg State University, 199034 St Petersburg, Russia
Interests: natural GMOs; agrobacterium; horizontal gene transfer

Special Issue Information

Dear Colleagues,

We are pleased to invite you to take part in the work of the 3rd International Conference “Genetically modified organisms: the Нistory, Achievements, Social and Environmental Risks”, which will be held on October 3-5, 2023, at St. Petersburg State University in a mixed format (full-time and remote participation). https://events.spbu.ru/events/gmo-2023?lang=Eng

This Special Issue will publish selected papers from the conference.

The conference will include several sections which cover the most significant research in the genetic engineering of plants. Topics of interest include, but are not limited to:

  • GMOs for fundamental research;
  • Plant genome editing technologies;
  • GMOs for agriculture;
  • GMOs for medicine;
  • GMOs and the environment;
  • GMOs and society.

Prof. Dr. Tatiana Matveeva
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • GMO
  • naturally transgenic plants
  • genome editing
  • metabolic engineering
  • agrobiotechnology
  • “Agrobacterium”

Published Papers (4 papers)

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Research

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11 pages, 2717 KiB  
Article
Genetically Modified Legume Plants as a Basis for Studying the Signal Regulation of Symbiosis with Nodule Bacteria
by Andrey D. Bovin, Alexandra V. Dolgikh, Alina M. Dymo, Elizaveta S. Kantsurova, Olga A. Pavlova and Elena A. Dolgikh
Horticulturae 2024, 10(1), 9; https://doi.org/10.3390/horticulturae10010009 - 21 Dec 2023
Cited by 1 | Viewed by 832
Abstract
The development of legume–rhizobial symbiosis results in the formation of nitrogen-fixing root nodules. In response to rhizobial molecules, Nod factors, signal transduction is mediated by the interaction of activated receptors with downstream signaling proteins. Previously, some new regulators of the signal pathway, such [...] Read more.
The development of legume–rhizobial symbiosis results in the formation of nitrogen-fixing root nodules. In response to rhizobial molecules, Nod factors, signal transduction is mediated by the interaction of activated receptors with downstream signaling proteins. Previously, some new regulators of the signal pathway, such as phospholipases D, which regulate the level of phosphatidic acid (PA), as well as mitogen-activated protein kinases (MAPKs), have been identified in legumes. Since PA is an important signal messenger, we tested the hypothesis that increasing the level of proteins involved in the reversible binding of PA in plant tissues may have a positive effect on symbiosis. Our findings showed that overexpression of MtSPHK1-PA, encoding the PA-binding domain of sphingosine kinase 1 (SPHK1), stimulated plant growth and nodule development in legume plants. Furthermore, the influence of MAPK6 on the development of symbiosis was studied. Using genetic engineering methods, we increased MAPK6 transcriptional activity in transgenic roots, leading to an increase in the number of nodules and the biomass of pea plants. Therefore, new approaches to obtain plants with an increased efficiency of symbiosis were tested. We report here that both genes that encode signaling proteins may be used as potential targets for future modification using biotechnological approaches. Full article
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11 pages, 1896 KiB  
Article
Phloem-Expressed CLAVATA3/ESR-like Genes in Potato
by Maria S. Gancheva, Maxim R. Losev, Irina E. Dodueva and Lyudmila A. Lutova
Horticulturae 2023, 9(12), 1265; https://doi.org/10.3390/horticulturae9121265 - 24 Nov 2023
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Abstract
In potato, phloem tissues transport sugars and signal molecules to the tuber for growth and storage. The CLAVATA3/ESR-like (CLE) family of plant peptides plays an important role in regulating plant development. In this study, we identified a set of phloem-expressed CLE genes in [...] Read more.
In potato, phloem tissues transport sugars and signal molecules to the tuber for growth and storage. The CLAVATA3/ESR-like (CLE) family of plant peptides plays an important role in regulating plant development. In this study, we identified a set of phloem-expressed CLE genes in Solanum tuberosum L. (StCLEs). We analyzed the phloem transcriptome of potato and found that 10 out of 41 StCLE genes were expressed in phloem cells, with StCLE12 and StCLE19 showing the highest expression levels. StCLE12 has an identical CLE domain to the Arabidopsis TDIF peptides, which are known to play a crucial role in maintaining the vascular meristem. StCLE19 has the highest sequence similarity to the Arabidopsis CLE25 peptide, which is involved in the formation of the phloem element and signaling in response to dehydration stress. The overexpression of StCLE12 and another potato TDIF-like gene, StCLE8, promoted vascular cell proliferation and delayed leaf senescence. On the other hand, plants with overexpression of StCLE19 were unable to form adventitious roots and demonstrated the absence of ordered cambium cell layers in the vascular bundles. Full article
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Review

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21 pages, 373 KiB  
Review
Crop Improvement: Comparison of Transgenesis and Gene Editing
by Natalya V. Permyakova and Elena V. Deineko
Horticulturae 2024, 10(1), 57; https://doi.org/10.3390/horticulturae10010057 - 06 Jan 2024
Viewed by 1377
Abstract
The development and improvement of molecular biology methods have led to the creation of new technologies that make it possible to modify plant genomes by transferring and integrating into the genomes’ heterologous genes from various expression systems (genetic engineering), as well as inducing [...] Read more.
The development and improvement of molecular biology methods have led to the creation of new technologies that make it possible to modify plant genomes by transferring and integrating into the genomes’ heterologous genes from various expression systems (genetic engineering), as well as inducing knockouts of one or more target genes of interest (genomic editing). The development of genome-editing methods is a new milestone in the development of modern breeding methods and certainly relies on the knowledge and technologies developed for transgenesis. This review will discuss issues related to the advantages and disadvantages of both technologies for improving the economically valuable traits of important crops. Full article
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11 pages, 511 KiB  
Review
The Search of a Molecular “Swiss Knife” for Chloroplast Genomic Editing
by Natalya V. Dorogova and Yuriy V. Sidorchuk
Horticulturae 2023, 9(12), 1338; https://doi.org/10.3390/horticulturae9121338 - 14 Dec 2023
Cited by 1 | Viewed by 763
Abstract
In recent years, genome editing methods have become an integral part of the genetic engineering toolset that allows for making targeted changes to plant genomes, both in the case of single-gene mutations and multiplex modifications. These technologies were mostly proven effective for editing [...] Read more.
In recent years, genome editing methods have become an integral part of the genetic engineering toolset that allows for making targeted changes to plant genomes, both in the case of single-gene mutations and multiplex modifications. These technologies were mostly proven effective for editing nuclear genomes. However, plastids, the best-known example of which is chloroplasts, have their own genome (plastome), which is also available for various genetic manipulations, including editing. Despite the fact that the modification of plastomes represents a very promising task for modern biotechnology, the structure of plastids and the peculiarities of their genome organization require the specific adaptation of genome editing methods. This applies to both the design of genetic constructs and methods of their delivery to plastids. The article provides an overview of the current state of research in the field of plastid genome editing with chloroplasts taken as an example. We consider the possibilities of using programmable genome-editing technologies, analyze their effectiveness, limitations, and problems caused by the structural features of these organelles, and their genome organization. We discuss the results of the first successful experiments in this field and try to assess the prospects for the development of tools and methods for increasing the efficiency and the specificity of this biotechnological platform. Full article
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