Microbial Genome Engineering and Synthetic Biology

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Microbial Genetics and Genomics".

Deadline for manuscript submissions: closed (15 March 2023) | Viewed by 1842

Special Issue Editors

School of Biological Science and Engineering, South China University of Technology, Guangzhou 510640, China
Interests: antimicrobials; biofilm control; biofilm-associated proteins; exopolysaccharides
Special Issues, Collections and Topics in MDPI journals
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139 USA
Interests: genome editing; metabolic engineering; recombineering; microbial products; pathway engineering; heterologous protein expression; protein engineering
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Synthetic biology has enabled us to engineer microbial cells for useful purposes by rewriting and editing their genomes. With the emergence of a variety of  powerful genome engineering techniques such as CRISPR/Cas systems, our ability to engineer microbial genomes has substantially increased in recent years. However, rationally designing and reshaping a genome to produce the desired phenotype remains enormously difficult, and more genome engineering strategies need to be explored, especially for non-model microorganisms that have potential useful applications.

This Special issue will cover the latest trends and developments of genome engineering tools and other synthetic biology techniques, and their application to give rise to improved or novel phenotypes in microorganisms, especially in non-model species. Original research or review papers are highly welcome for submission on the following topics: CRISPR/Cas-based genome editing methods, recombineering techniques, methodologies for genome-scale engineering, development of programmable chassis, multiplex genome editing, metabolic engineering, novel genome-modification tools, genome engineering in non-model microbes.

 

Dr. Yanrui Ye
Dr. Cheng Li
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Genome editing
  • Recombineering
  • Metabolic engineering
  • Pathway refactoring
  • Microbial cell factories
  • CRISPR/Cas
  • Non-model microbes
  • Synthetic pathway
  • Microbial chassis

Published Papers (1 paper)

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Research

11 pages, 2649 KiB  
Article
Biotransformation of High Concentrations of Ginsenoside Substrate into Compound K by β-glycosidase from Sulfolobus solfataricus
by Pan Wang, Congcong Tang, Yannan Liu, Jing Yang and Daidi Fan
Genes 2023, 14(4), 897; https://doi.org/10.3390/genes14040897 - 12 Apr 2023
Cited by 4 | Viewed by 1282
Abstract
The rare ginsenoside Compound K (CK) is an attractive ingredient in traditional medicines, cosmetics, and the food industry because of its various biological activities. However, it does not exist in nature. The commonly used method for the production of CK is enzymatic conversion. [...] Read more.
The rare ginsenoside Compound K (CK) is an attractive ingredient in traditional medicines, cosmetics, and the food industry because of its various biological activities. However, it does not exist in nature. The commonly used method for the production of CK is enzymatic conversion. In order to further improve the catalytic efficiency and increase the CK content, a thermostable β-glycosidase from Sulfolobus solfataricus was successfully expressed in Pichia pastoris and secreted into fermentation broth. The recombinant SS-bgly in the supernatant showed enzyme activity of 93.96 U/mg at 120 h when using pNPG as substrate. The biotransformation conditions were optimized at pH 6.0 and 80 °C, and its activity was significantly enhanced in the presence of 3 mM Li+. When the substrate concentration was 10 mg/mL, the recombinant SS-bgly completely converted the ginsenoside substrate to CK with a productivity of 507.06 μM/h. Moreover, the recombinant SS-bgly exhibited extraordinary tolerance against high substrate concentrations. When the ginsenoside substrate concentration was increased to 30 mg/mL, the conversion could still reach 82.5% with a productivity of 314.07 μM/h. Thus, the high temperature tolerance, resistance to a variety of metals, and strong substrate tolerance make the recombinant SS-bgly expressed in P. pastoris a potential candidate for the industrial production of the rare ginsenoside CK. Full article
(This article belongs to the Special Issue Microbial Genome Engineering and Synthetic Biology)
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