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Genes
Special Issues
Fish and Shellfish Genetics and Breeding
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Fish and Shellfish Genetics and Breeding

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Animal Genetics and Genomics".

Deadline for manuscript submissions: closed (25 August 2023) | Viewed by 2751

Special Issue Editors

Prof. Dr. Xianhu Zheng

E-Mail Website
Guest Editor
National Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China
Interests: fish genetics; molecular breeding; aquatic germplasm resources evaluation and utilization
Dr. Shubo Jin

E-Mail Website
Guest Editor
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Interests: aquaculture; breeding; transcriptome; genes; SNP
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

[*] Background & history of this topic:

Aquaculture has experienced rapid growth over the past few decades, one of the major factors being the rapid development of genetic breeding techniques for aquaculture. Aquatic genetic breeding is an effective tool to analyze the genetic mechanisms of complex phenotypes in fish and shellfish, and to create new aquatic varieties with good economic traits such as high yield, disease resistance or stress resistance. It plays an important role in improving the yield and quality of aquatic products.

In recent years, with the extensive development of economic fish and shellfish genome resources, emerging technologies such as sex control, chromosome manipulation, genomic selection, genome editing and big data technology have been rapidly applied to the genetic breeding of fish and shellfish. The aquaculture seed industry has undergone dramatic and novel changes, and there is an urgent need to integrate and share these new developments with peers.

[*] Aim and scope of the Special Issue:

The aim of this Special Issue is to present innovations and improvements of modern aquatic breeding technological advances, such as genetic sex control, genomic selection, genome editing and high-throughput gene chip production. At the same time, the creation of excellent strains is also a priority, along with the preservation and innovation of germplasm resources, as well as the breakthrough of the above new technologies in fish and shellfish, in order to cultivate new strains with high yield, disease resistance, stress resistance and high quality, to meet the requirements of different farming modes and environments.

[*] Cutting-edge research:

  1. Characterization of aquatic genetic resources (AqGr) for conservation and sustainable use in breeding programs.
  2. Genetic architecture analysis of complex traits.
  3. Genome-wide association analysis.
  4. Marker-assisted selection.
  5. Genomic selection.
  6. Gene editing applied to breeding programs.
  7. Non-destructive and rapid approach of high-throughput phenotyping.
  8. Development of software tools for genetic breeding of fish and shellfish.

[*] What kinds of papers we are soliciting:

Research articles, communications, and reviews will be accepted for publication.

Prof. Dr. Xianhu Zheng
Dr. Shubo Jin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • gene function
  • genomics
  • QTL
  • GWAS
  • marker-assisted selection
  • genomic selection
  • gene editing

Published Papers (2 papers)

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Research

15 pages, 3940 KiB  
Article
Mn-XRN1 Has an Inhibitory Effect on Ovarian Reproduction in Macrobrachium nipponense
by Tianyong Chen, Huwei Yuan, Hui Qiao, Sufei Jiang, Wenyi Zhang, Yiwei Xiong, Hongtuo Fu and Shubo Jin
Genes 2023, 14(7), 1454; https://doi.org/10.3390/genes14071454 - 16 Jul 2023
Cited by 1 | Viewed by 985
Abstract
XRN1 is an exoribonuclease that degrades mRNA in the cytoplasm along the 5′–3′ direction. A previous study indicated that it may be involved in the reproduction of Macrobrachium nipponense. Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of Mn-XRN1 [...] Read more.
XRN1 is an exoribonuclease that degrades mRNA in the cytoplasm along the 5′–3′ direction. A previous study indicated that it may be involved in the reproduction of Macrobrachium nipponense. Quantitative real-time PCR was used to detect the spatiotemporal expression pattern of Mn-XRN1. At the tissue level, Mn-XRN1 was significantly expressed in the ovary. During development, Mn-XRN1 was significantly expressed at the CS stage of the embryo, on the 10th day post-larval and in the O2 stage of ovarian reproduction. The in situ hybridization results showed the location of Mn-XRN1 in the ovary. The expression of Mn-VASA was significantly increased after in vivo injection of Mn-XRN1 dsRNA. This suggests that Mn-XRN1 negatively regulates the expression of Mn-VASA. Furthermore, we counted the number of M. nipponense at various stages of ovarian reproduction on different days after RNAi. The results showed that ovarian development was significantly accelerated. In general, the results of the present study indicate that Mn-XRN1 has an inhibitory effect on the ovarian maturation of M. nipponense. The inhibitory effect might be through negative regulation of Mn-VASA. Full article
(This article belongs to the Special Issue Fish and Shellfish Genetics and Breeding)
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15 pages, 3528 KiB  
Article
Cloning, Expression Analysis and SNP Screening of the kiss1 Gene in Male Schizothorax biddulphi
by Zhulan Nie, Nianhua Zhao, He Zhao, Zhengyi Fu, Zhenhua Ma and Jie Wei
Genes 2023, 14(4), 862; https://doi.org/10.3390/genes14040862 - 03 Apr 2023
Cited by 1 | Viewed by 1194
Abstract
Schizothorax biddulphi is an endemic fish distributed only in southern Xinjiang, China. Due to overfishing, water conservancy facilities, and other factors, as well as inherent biological limitations, resource recovery is quite difficult. For endangered fish with slow growth, late sexual maturity, and insufficient [...] Read more.
Schizothorax biddulphi is an endemic fish distributed only in southern Xinjiang, China. Due to overfishing, water conservancy facilities, and other factors, as well as inherent biological limitations, resource recovery is quite difficult. For endangered fish with slow growth, late sexual maturity, and insufficient natural population supplementation, large-scale artificial reproduction and breeding are important for restoring resources. Therefore, it is urgent to optimize the reproductive regulation methods of the fish. The kiss1 gene is a key regulator of the reproductive regulation cascade, and identifying and analyzing the role of kiss1 are important for further elucidating the reproductive mechanism of S. biddulphi. To understand the characteristics of the kiss1 of S. biddulphi, the full-length cDNA sequence of kiss1 was obtained in this study, and its tissue expression specificity and association with phenotypic traits were analyzed in male fish. The full-length cDNA sequence of kiss1 in S. biddulphi was 658 bp, with an ORF of 327 bp, and encoded a 108-amino acid, unstable protein. Homology results indicated that kiss1 was highly conserved. qPCR showed kiss1 expression in different tissues in male S. biddulphi, with the highest expression in the gonads, followed by muscle, and significantly lower expression in the swim bladder, pituitary gland, heart, hypothalamus, gill, fin, liver, eye, and mid-kidney. qPCR revealed three SNP loci in the exonic region of kiss1. The c.3G>T locus was significantly correlated (p < 0.05) with gonad mass and the maturation coefficient in S. biddulphi. These results will help uncover the reproductive endocrinology network of S. biddulphi, improve artificial breeding technology for fish, and unveil new directions for breeding excellent strains of S. biddulphi and molecular marker-assisted breeding. Full article
(This article belongs to the Special Issue Fish and Shellfish Genetics and Breeding)
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