Enzymes in Food Processing

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Engineering and Technology".

Deadline for manuscript submissions: closed (25 June 2023) | Viewed by 4587

Special Issue Editors


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Guest Editor
Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok, Thailand
Interests: extraction, characterization, and application of plant enzyme; bioactive compounds; biodegradable polyesters

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Guest Editor
Food Technology Program, Kanchanaburi Campus, Mahidol University, Kanchanaburi, Thailand
Interests: food science and technology; enzymatic process; bioactive compounds; bio-based materials and packaging; biodegradation of polyesters

Special Issue Information

Dear Colleagues,

Both conventional and novel food products can be obtained from the use of enzymes. Natural enzymes have been employed to make foods such as cheese, beer, wine, and vinegar since the beginning of human civilization. Their role in food processing, including in the manufacturing of soy sauce, the tenderization of meat, the production of cheese, and in baking and brewing, has also been acknowledged for many centuries. Enzymes also provide a comprehensive and varied selection of specialized tools for the preparation of food and beverages. They can work incredibly effectively to reduce the usage of chemical food additives, extend shelf life, enrich flavour, improve texture, and control process time. Complex molecules are broken down by enzymes into smaller molecules, such as sugar from carbohydrates. In addition to their great reputation, enzymes have the advantages of high activity, specificity, and cost-effectiveness, which makes them the most affordable choice in those areas of food processing. Being naturally occurring substances, enzymes are, therefore, always preferred by consumers. Recently, environmental concerns and consumer demands are driving the need for alternative food production methods, including for future food.

In this Special Issue, we invite researchers to contribute their original or review articles related to enzymes in food processing. The use of enzymes to tailor the desirable physicochemical and biological properties of food products in food processing and the food industry is awaiting new research and development in this area. 

Prof. Dr. Pilanee Vaithanomsat
Prof. Dr. Amnat Jarerat
Guest Editors

Manuscript Submission Information

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Keywords

  • alcoholic and nonalcoholic beverages
  • fat and oil processing
  • cereal and grain processing
  • bakery and confectionary products
  • sugar and sweetener processing
  • fermented foods
  • marine and fishery products
  • meat and poultry products
  • fruit and vegetables
  • future food
  • enzymes

Published Papers (2 papers)

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Research

14 pages, 1766 KiB  
Article
Short-Chain Fructooligosaccharide Synthesis from Sugarcane Syrup with Commercial Enzyme Preparations and Some Physical and Antioxidation Properties of the Syrup and Syrup Powder
by Sudthida Kamchonemenukool, Warathep Buasum, Monthana Weerawatanakorn and Tipawan Thongsook
Foods 2023, 12(15), 2895; https://doi.org/10.3390/foods12152895 - 29 Jul 2023
Cited by 1 | Viewed by 1294
Abstract
Short-chain fructooligosaccharides (sc-FOS) are prebiotics beneficial to human health, which can be synthesized from raw material containing a high sucrose content. Sugarcane syrup (SS) without molasses removal contains sucrose as a major sugar and is a rich source of several bioactive compounds. The [...] Read more.
Short-chain fructooligosaccharides (sc-FOS) are prebiotics beneficial to human health, which can be synthesized from raw material containing a high sucrose content. Sugarcane syrup (SS) without molasses removal contains sucrose as a major sugar and is a rich source of several bioactive compounds. The aim of this study is to investigate factors affecting sc-FOS synthesis from SS using commercial enzyme preparations containing fructosyltransferase activity as biocatalysts. sc-FOS content increased significantly as the sucrose concentration of SS in the reaction mixture increased up to 40% (w/v). Changes in carbohydrate compositions during the transfructosylating reaction of a pure sucrose solution and SS prepared from the two sugarcane cultivars Khon Kaen 3 and Suphanburi 50, catalyzed by Pectinex Ultra SP-L and Viscozyme L, showed similar profiles. Both enzymes showed a high ability to transfer fructosyl moieties to produce sc-FOS and a plateau of the total sc-FOS concentration was observed after 4 h of reaction time. For the pure sucrose solution and SS (Suphanburi 50), Viscozyme showed a superior ability to convert sucrose to Pectinex, with a higher sc-FOS yield (g FOS/100 g of initial sucrose), GF2 or 1-kestose yield (g GF2/g of initial sucrose) and GF3 or nystose yield (g GF3/g of initial sucrose). sc-FOS syrup (FOS SS) and the foam-mat-dried syrup powder prepared from SS and FOS SS, respectively, contained a high total phenolic content and possessed higher antioxidant activities than those prepared from pure sucrose, but contained lower calories. Full article
(This article belongs to the Special Issue Enzymes in Food Processing)
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18 pages, 2545 KiB  
Article
Production of a Series of Long-Chain Isomaltooligosaccharides from Maltose by Bacillus subtilis AP-1 and Associated Prebiotic Properties
by Suratsawadee Tiangpook, Sreyneang Nhim, Pattaneeya Prangthip, Patthra Pason, Chakrit Tachaapaikoon, Khanok Ratanakhanokchai and Rattiya Waeonukul
Foods 2023, 12(7), 1499; https://doi.org/10.3390/foods12071499 - 03 Apr 2023
Cited by 3 | Viewed by 2246
Abstract
Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity, was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of polymerization (DP) ranging from 2 to 14 by direct fermentation of maltose. A total IMOs yield of 36.33 g/L without [...] Read more.
Bacillus subtilis strain AP-1, which produces α-glucosidase with transglucosidase activity, was used to produce a series of long-chain isomaltooligosaccharides (IMOs) with degree of polymerization (DP) ranging from 2 to 14 by direct fermentation of maltose. A total IMOs yield of 36.33 g/L without contabacillusmination from glucose and maltose was achieved at 36 h of cultivation using 50 g/L of maltose, with a yield of 72.7%. IMOs were purified by size exclusion chromatography with a Superdex 30 Increase column. The molecular mass and DP of IMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Subsequently, linkages in produced oligosaccharides were verified by enzymatic hydrolysis with α-amylase and oligo-α-1,6-glucosidase. These IMOs showed prebiotic properties, namely tolerance to acidic conditions and digestive enzymes of the gastrointestinal tract, stimulation of probiotic bacteria growth to produce short-chain fatty acids and no stimulating effect on pathogenic bacteria growth. Moreover, these IMOs were not toxic to mammalian cells at up to 5 mg/mL, indicating their biocompatibility. Therefore, this research demonstrated a simple and economical method for producing IMOs with DP2–14 without additional operations; moreover, the excellent prebiotic properties of the IMOs offer great prospects for their application in functional foods. Full article
(This article belongs to the Special Issue Enzymes in Food Processing)
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