Special Issue "C1 Based Microbial Cell Factory"

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Microbial Metabolism, Physiology & Genetics".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 2075

Special Issue Editors

College of Food and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China
Interests: synthetic biology; microbial consortium; genetic system development; metabolic engineering; biochemicals
Environmental Sciences Department, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia
Interests: synthetic biology; Clostridum; biofuel; metabolic engineering; butanol

Special Issue Information

Dear Colleagues,

In the past two years, the coronavirus disease 2019 pandemic led to a huge negative impact on global crude oil production and transportation; on the other hand, the economic recovery of some countries continued to increase the demand for crude oil. Most recently, the international political situation has led to a sharp rise in crude oil prices. In addition, as many countries’ policies to address climate change come into effect, carbon emission have gradually tightened. Therefore, the cheap and renewable one carbon (C1) substrates (including CO, CO2, methane, methanal, methanoic acid, methanol) have generated increasing attention as abundantly available feedstock for biotransformation to produce biofuels and valuable chemicals. Developing C1-based microbial cell factories, such as Escherichia coli, Corynebacterium, methanogens, brewer's yeast, clostridia, oleaginous yeast, and algae, is meaningful and of great significance. 

Unfortunately, the performance of the C1-based microbial cell factory, including substrate utilization capacity, product synthesis efficiency (i.e., titer, yield, and productivity), and tolerance to inhibitors and products are still putative bottlenecks toward industrial production and commercialization.

We have noted recent breakthroughs of synthetic biotechnology in genome editing tools development, and the acquisition and analysis of massive omics data have made it possible to recruit genetic elements across species and reshape chassis cells at the molecular level. Accordingly, the metabolic pathways or functional modules could be restructured, debugged, and applied in classic and even some non-model strains. More importantly, the synthetic microbial community learning from natural symbiosis phenomena holds great promise in syngas biofixation and C1 compound utilization through the division of metabolic labor and construction of cross-species metabolic pathways.

The overall goal of this Special Issue is to gather recent progress in C1-based microbial cell factory development driven by synthetic biotechnology and other technique. The scope of the special issue covers new discoveries, strategies, and methods to accelerate product development, feedstock utilization, strains performance optimization, and synthetic consortium regulation. This topic is committed to the publication of original research and review articles.

Dr. Zhiqiang Wen
Dr. Naief H. Al Makishah
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • C1 compounds
  • fermentation
  • metabolic engineering
  • genetic system
  • microbial cell factory
  • synthetic biology
  • bioreactor
  • syngas
  • biofixation
  • carbon-neutral

Published Papers (1 paper)

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Conversion of Syngas from Entrained Flow Gasification of Biogenic Residues with Clostridium carboxidivorans and Clostridium autoethanogenum
Fermentation 2022, 8(9), 465; https://doi.org/10.3390/fermentation8090465 - 17 Sep 2022
Cited by 1 | Viewed by 1682
Synthesis gas fermentation is a microbial process, which uses anaerobic bacteria to convert CO-rich gases to organic acids and alcohols and thus presents a promising technology for the sustainable production of fuels and platform chemicals from renewable sources. Clostridium carboxidivorans and Clostridium autoethanogenum [...] Read more.
Synthesis gas fermentation is a microbial process, which uses anaerobic bacteria to convert CO-rich gases to organic acids and alcohols and thus presents a promising technology for the sustainable production of fuels and platform chemicals from renewable sources. Clostridium carboxidivorans and Clostridium autoethanogenum are two acetogenic bacteria, which have shown their high potential for these processes by their high tolerance toward CO and in the production of industrially relevant products such as ethanol, 1-butanol, 1-hexanol, and 2,3-butanediol. A promising approach is the coupling of gasification of biogenic residues with a syngas fermentation process. This study investigated batch processes with C. carboxidivorans and C. autoethanogenum in fully controlled stirred-tank bioreactors and continuous gassing with biogenic syngas produced by an autothermal entrained flow gasifier on a pilot scale >1200 °C. They were then compared to the results of artificial gas mixtures of pure gases. Because the biogenic syngas contained 2459 ppm O2 from the bottling process after gasification of torrefied wood and subsequent syngas cleaning for reducing CH4, NH3, H2S, NOX, and HCN concentrations, the oxygen in the syngas was reduced to 259 ppm O2 with a Pd catalyst before entering the bioreactor. The batch process performance of C. carboxidivorans in a stirred-tank bioreactor with continuous gassing of purified biogenic syngas was identical to an artificial syngas mixture of the pure gases CO, CO2, H2, and N2 within the estimation error. The alcohol production by C. autoethanogenum was even improved with the purified biogenic syngas compared to reference batch processes with the corresponding artificial syngas mixture. Both acetogens have proven their potential for successful fermentation processes with biogenic syngas, but full carbon conversion to ethanol is challenging with the investigated biogenic syngas. Full article
(This article belongs to the Special Issue C1 Based Microbial Cell Factory)
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