Special Issue "Recent Trends in High-Cell-Density Fermentation Strategies for Microbial Lipids"

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Microbial Metabolism, Physiology & Genetics".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 1130

Special Issue Editors

Director of Colin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Science, Shandong University of Technology, 266 Xincun Rd., Zibo, China
Interests: molecular mechanisms of lipid biosynthesis; biochemistry; microbial lipids; M. circinelloides; genetic engineering; fatty acids synthesis; biomass and value-added products; chemical engineering; fermentation biotechnology; food chemistry; functional foods; applied microbiology; natural products
Special Issues, Collections and Topics in MDPI journals
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Interests: genetics

Special Issue Information

Dear Colleagues,

Recently, there has been considerable concern over the traditional fermentation process and nutritional problems accompanying the rapid growth of the world population and the current economic situation. Thus, it is necessary to find an effective and suitable fermentation process for lipid production. Oleaginous microorganisms, including fungi, yeasts, algae, bacteria, etc., are widely used in the fermentation industry, and they have been considered powerful oil sources for lipid and biodiesel production based on their ability to accumulate large amounts of lipids. The major challenges in fermentation production systems are low reaction rates and high capital costs. High-cell-density culture is an effective method to enhance the volumetric productivity of the fermentation process, thus making the process faster and more robust. High levels of cell biomass in defined media can be easily achieved; therefore, large quantities of recombinant products with enhanced activities at lower cost can be obtained through high-cell-density fermentation technology. The development of microbial strains and their fermentation process has been implemented to produce significant lipids and multiple significant fatty acids with nutritional importance.

However, to develop efficient fermentation technologies for biomass, lipid, and biofuel production, more significant research attention needs to be directed toward biotechnological and genetic improvement of the potential organisms utilized in the process by using useful, varied fermentation strategies.

In this Special Issue, we will cover some recent progress focused on novel fermentation methods, molecular techniques, and the development of additional microbial models for varied biotechnological applications and their ability to modulate, regulate, and maximize production through high-cell-density fermentation strategies. We aim to (a) provide an introductory overview of microbial lipids and to show the current attention being paid to their production and the use of oleaginous microorganisms as powerful sources of oils and fats, (b) high-throughput screening technologies for industrially important lipids and fatty-acid-producing microorganisms under special fermentation models, (c) highlight novel fermentation methods and trends proposed to produce high biomass and lipids and lipid derivatives in sufficient quantities by targeting microorganisms, (d) provide an overview of discovered model species and their fermentation approaches, and (e) give insights into the molecular mechanisms of stress response and tolerance during various fermentation strategies.

Prof. Dr. Yuanda Song
Dr. Wanwipa Vongsangnak
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • oleaginous microorganisms
  • microbial lipids
  • lipid biosynthesis
  • fungi
  • yeasts
  • bacteria
  • fed-batch fermentation
  • batch fermentation
  • genetic manipulation
  • lipid biochemistry
  • fatty acids
  • lipid accumulation
  • biofuels
  • biotechnology

Published Papers (1 paper)

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14 pages, 1976 KiB  
Construction of Eicosatetraenoic Acid Producing Cell Factory by Genetic Engineering of Mucor circinelloides
Fermentation 2023, 9(7), 653; https://doi.org/10.3390/fermentation9070653 - 12 Jul 2023
Viewed by 829
Eicosatetraenoic acid (ETA, 20:4, ω-3) is the desaturation product of dihomo-gamma linolenic acid (DGLA, 20:3, ω-6) catalyzed by delta-17 desaturase, which is considered as a healthy product that helps to lower risk of heart diseases. The oleaginous filamentous fungus, Mucor circinelloides, has [...] Read more.
Eicosatetraenoic acid (ETA, 20:4, ω-3) is the desaturation product of dihomo-gamma linolenic acid (DGLA, 20:3, ω-6) catalyzed by delta-17 desaturase, which is considered as a healthy product that helps to lower risk of heart diseases. The oleaginous filamentous fungus, Mucor circinelloides, has been used for a long time as a model micro-organism for GLA production at industrial scales. However, M. circinelloides lacks the key enzymes to synthesize C20 polyunsaturated fatty acids (PUFAs). M. circinelloides could produce DGLA by overexpressing the D6E(GLELO) gene, which could be a useful tool to produce ETA due to the availability of established genetic manipulation tools. Therefore, in this study, delta-17 desaturase (PpD17 and PaD17) genes from Phytophthora parasitica and Pythium aphanidermatum, respectively, were introduced into M. circinelloides to construct an ETA-producing cell factory. Our results showed that the PaD17 and PpD17 overexpression strains’ biomass increased by 25.98 and 23.34 g/L (39.98 and 25.75%), respectively, compared with the control strain. Meanwhile, the lipid contents of the recombinant strains also increased and reached up to 28.88% in Mc-PaD17 and 30.95% in Mc-PpD17, respectively, compared with the control strain (23.38% in Mc-2076). The RT-qPCR results showed that overexpression of delta-17 desaturase genes promoted the expression of cme2, fas2, and D6E, thereby contributing to lipid biosynthesis in M. circinelloides. Meanwhile, the content of ETA reached up to 1.95%, and the yield of ETA was up to 114.69 mg/L in PpD17 overexpression mutants at 96 h. This study provided the first report on the construction of an ETA-producing cell factory by heterologous overexpression of the PpD17 gene in M. circinelloides, which established a new scope for further research in the production of ETA in oleaginous fungi. Full article
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