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Fermentation
Special Issues
Separation and Purification of Biosynthetic Products
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Separation and Purification of Biosynthetic Products

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Fermentation Process Design".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 5038

Special Issue Editor

Dr. Lenuta Kloetzer

E-Mail Website
Guest Editor
"Cristofor Simionescu" Faculty of Chemical Engineering and Environmental Protection, “Gheorghe Asachi” Technical University, D. Mangeron 73, 700050 Iasi, Romania
Interests: extraction; membranes; separation science; solvent extraction; high pressure liquid chromatography; method development; fermentation

Special Issue Information

Dear Colleagues,

Growing demand of obtaining natural or biosynthetic compounds with applications in various fields, like pharmaceutical, food, cosmetics, chemical processes, fuel, and environmental engineering, led to continuous research for the development of separation and purification technologies. Biosynthesis or enzymatic processes, involving the use of microorganisms or enzymes for biochemical transformations, allow the production of a wide range of essential compounds, biologically active. The current economic impact of chemical products obtained by fermentation is still limited, mainly due to difficulties in recovering the product and the efficiency of a biotechnological process is correlated with the stages of product separation.

This Special Issue titled “Separation and Purification of Biosynthetic Products” aims to cover recent advances in methods and compounds used for the separation and purification of natural or biosynthetic products.

In this Special Issue, original research articles and reviews are welcome.

We look forward to receiving your contributions.

Dr. Lenuta Kloetzer
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • liquid-liquid extraction
  • biosynthetic products
  • bioactive compounds
  • membrane separation
  • downstream processing
  • bioseparation
  • ionic liquids
  • biotechnology
  • fermentation

Published Papers (3 papers)

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Research

15 pages, 1911 KiB  
Article
Application of Aqueous Two-Phase Systems with Thermoseparating Polymers (EOPO) as a Method for Extractive Fermentation with Neochloris oleoabundans
by Ana Patricia Garza-Chapa, Carlos Iván Ávila-Velasco, José González-Valdez and Alma Gómez-Loredo
Fermentation 2024, 10(3), 130; https://doi.org/10.3390/fermentation10030130 - 25 Feb 2024
Cited by 1 | Viewed by 914
Abstract
Extractive fermentation is an in situ method for the production and recovery of biomolecules of interest. Aqueous two-phase systems (ATPS) allow the product to be recovered in one phase of the system, reducing unit operations in the bioprocess. Thermosensitive polymers such as EOPOs [...] Read more.
Extractive fermentation is an in situ method for the production and recovery of biomolecules of interest. Aqueous two-phase systems (ATPS) allow the product to be recovered in one phase of the system, reducing unit operations in the bioprocess. Thermosensitive polymers such as EOPOs are an interesting alternative to be applied in ATPS. In this work, different EOPOs were tested in an extractive fermentation strategy with the green microalgae Neochloris oleoabundans to provide a basis for future implementations of these systems in microalgae bioprocesses. Extractive fermentations were carried out with two EOPOs of different molecular weights (3900 and 12,000 g/mol) at concentrations of 10% and 15% (w/v). The microalga was incubated axenically under two different sets of conditions for 21 and 45 days, respectively. Cell counts were performed, and cell growth curves were obtained. Additionally, a semi-continuous and batch extractive fermentation assay was performed. The extractive fermentation with EOPO showed lower cell growth and a longer adaptation time of the microalgae in the fermentation, and EPS production yields of up to 8–23 g/L were obtained. Extractive fermentation is an interesting method to be implemented in microalgae cultures; however, further conditions need to be explored to achieve an appropriate bioprocess. Full article
(This article belongs to the Special Issue Separation and Purification of Biosynthetic Products)
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15 pages, 2266 KiB  
Article
Efficient Purification of 2′-Fucosyllactose by Membrane Filtration and Activated Carbon Adsorption
by Yuanyifei Wang, Jiaqiang Hu, Mengyu Sun, Bo Peng and Shuo Wang
Fermentation 2022, 8(11), 655; https://doi.org/10.3390/fermentation8110655 - 19 Nov 2022
Cited by 1 | Viewed by 1643
Abstract
With the rapid development of synthetic biology, the production of 2′-fucosyllactose by biological fermentation gradually has the basis for industrialization. However, the lack of efficient downstream technology of biological fermentation, especially purification technology, has become the main factor limiting its commercialization. In this [...] Read more.
With the rapid development of synthetic biology, the production of 2′-fucosyllactose by biological fermentation gradually has the basis for industrialization. However, the lack of efficient downstream technology of biological fermentation, especially purification technology, has become the main factor limiting its commercialization. In this study, based on the general E. coli biosynthesis of 2′-fucosyllactose fermentation broth, most of the impurities were removed and concentrated using membrane filtration technology after simple flocculation. The target 2′-fucosyllactose was eluted in a targeted manner using activated carbon adsorption and ethanol gradient elution technology. The 2′-fucosyllactose product with 90% or even higher purity could be prepared efficiently. This study explored a new direction for the industrial production of 2′-fucosyllactose. Full article
(This article belongs to the Special Issue Separation and Purification of Biosynthetic Products)
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Graphical abstract

14 pages, 2090 KiB  
Article
Gallic Acid Reactive Extraction with and without 1-Octanol as Phase Modifier: Experimental and Modeling
by Alexandra Cristina Blaga, Elena Niculina Dragoi, Raluca Elena Munteanu, Dan Cascaval and Anca Irina Galaction
Fermentation 2022, 8(11), 633; https://doi.org/10.3390/fermentation8110633 - 12 Nov 2022
Cited by 2 | Viewed by 1460
Abstract
Gallic acid (GA) is a naturally occurring phenolic acid that can be found in the leaves, roots, flowers, or stems of a wide variety of plant species. It has a broad range of uses in the food and pharmaceutical industries. The objective of [...] Read more.
Gallic acid (GA) is a naturally occurring phenolic acid that can be found in the leaves, roots, flowers, or stems of a wide variety of plant species. It has a broad range of uses in the food and pharmaceutical industries. The objective of this research is to investigate the GA reactive extraction process employing dichloromethane and n-heptane as solvents, 1-octanol as a phase-modifier, and Amberlite LA-2 as an amine extractant dissolved in the organic phase. The separation yield and distribution coefficient data were discussed, along with the analysis of the extraction conditions and the extraction mechanism. Dichloromethane employed as the solvent, 80 g/L Amberlite LA2 used as the extractant, and 10% phase modifier were determined to be the ideal conditions for the reactive extraction onto a biphasic organic-aqueous system. Statistical regression and artificial neural networks (ANNs) established with the differential evolution (DE) algorithm were also used to model and optimize the process. Full article
(This article belongs to the Special Issue Separation and Purification of Biosynthetic Products)
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