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Diagnostics
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The Role of Non-coding RNAs (ncRNAs) in Molecular Diagnosis of...
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The Role of Non-coding RNAs (ncRNAs) in Molecular Diagnosis of Diseases

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Pathology and Molecular Diagnostics".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 3058

Special Issue Editors

Dr. Manal S. Fawzy

E-Mail Website
Guest Editor
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt
Interests: molecular biology; non-coding RNA; gene expression; DNA polymorphism; cancer biology
Dr. Eman A. Toraih

E-Mail Website
Guest Editor
Division of Endocrine and Oncologic Surgery, Department of Surgery, Tulane University School of Medicine, New Orleans, LA 70112, USA
Interests: endocrine surgery; thyroid cancer; parathyroids; adrenals; melanoma; big data

Special Issue Information

Dear Colleagues,

Recent studies have unveiled the several roles of non-coding RNAs (ncRNAs), highlighting the biological significance of these previously 'overlooked' RNA families. ncRNAs and especially microRNAs (miRNAs), with an average of 22 nucleotides in length, and more recently, long ncRNAs (lncRNAs), more than 200 nucleotides in length, have arrived at the center of biological research, involved in a plethora of biological processes, including gene expression, chromatin remodeling, and cell cycle regulation, among others.

Given the high stability of ncRNAs in various body fluids and ease of access with a minimally invasive nature, these types of RNAs create good opportunities for the development of novel diagnostic strategies for various human diseases. Further, the essential role of ncRNAs-related variants is increasingly being demonstrated and proven to be implicated in disease risk/susceptibility.

This Special Issue will comprise original research and review articles focused on the role of all types of ncRNAs in the molecular diagnosis of human diseases.

Dr. Manal S. Fawzy
Dr. Eman A. Toraih
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • non-coding RNAs
  • lncRNA
  • miRNA
  • diagnosis
  • molecular profiling
  • gene expression
  • gene variants
  • In silico analysis

Published Papers (2 papers)

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Research

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21 pages, 5209 KiB  
Article
Molecular Characterization of Patients with Cryptorchidism: Preliminary Search for an Expression Profile Related to That of Testicular Germ-Cell Tumors
by Fabiola García-Andrade, Rosa María Vigueras-Villaseñor, Margarita Dolores Chávez-Saldaña, Julio César Rojas-Castañeda, Ivan Uriel Bahena-Ocampo, Elena Aréchaga-Ocampo, Mauricio Flores-Fortis, José Díaz-Chávez, Luis Alonso Herrera and Daniel Adrian Landero-Huerta
Diagnostics 2023, 13(18), 3020; https://doi.org/10.3390/diagnostics13183020 - 21 Sep 2023
Cited by 1 | Viewed by 1375
Abstract
Cryptorchidism (CO) is a risk factor for the development of testicular germ-cell tumors (TGCT). This is supported by reports showing the persistence of gonocytes in CO patients. These cells are proposed to be related to the development of germ-cell neoplasia in situ (GCNIS), [...] Read more.
Cryptorchidism (CO) is a risk factor for the development of testicular germ-cell tumors (TGCT). This is supported by reports showing the persistence of gonocytes in CO patients. These cells are proposed to be related to the development of germ-cell neoplasia in situ (GCNIS), which is considered the precursor stage/lesion of TGCT. Therefore, it is proposed that some patients with CO could express some molecular markers related to TGCT. In this study, we analyzed testicular tissue samples from CO, TGCT, and controls. We determined the expression of POU5F1, PLAP, and KIT by immunohistochemistry and that of the hsa-miR-371-373 cluster, hsa-miR-367, and LATS2, PTEN, and IGFR1 genes by RT-qPCR. We then carried out a bioinformatic analysis to identify other possible candidate genes as tumor biomarkers. We found that 16.7% (2/12) of the CO patients presented increased expression of POU5F1, KIT, PLAP, hsa-miR-371-373, and hsa-miR-367 and decreased expression of LATS2 and IGF1R. Finally, the genes ARID4B, GALNT3, and KPNA6 were identified as other possible candidate tumor biomarkers. This is the first report describing the expression of the hsa-miR-371-373 cluster, hsa-miR-367, LATS2, and IGF1R in the testicular tissues of two CO patients with cells immune-positive to POU5F1, PLAP, and KIT, which is similar to what is observed in TGCT. Full article
(This article belongs to the Special Issue The Role of Non-coding RNAs (ncRNAs) in Molecular Diagnosis of Diseases)
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Review

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16 pages, 349 KiB  
Review
PiRNA Obtained through Liquid Biopsy as a Possible Cancer Biomarker
by Piotr Limanówka, Błażej Ochman and Elżbieta Świętochowska
Diagnostics 2023, 13(11), 1895; https://doi.org/10.3390/diagnostics13111895 - 29 May 2023
Cited by 1 | Viewed by 1151
Abstract
In recent years PIWI-interacting RNAs (piRNAs) have gained the interest of scientists, mainly because of their possible implications in cancer. Many kinds of research showed how their expression can be linked to malignant diseases. However, most of them evaluated the expression of piRNAs [...] Read more.
In recent years PIWI-interacting RNAs (piRNAs) have gained the interest of scientists, mainly because of their possible implications in cancer. Many kinds of research showed how their expression can be linked to malignant diseases. However, most of them evaluated the expression of piRNAs in tumor tissues. It was shown how these non-coding RNAs can interfere with many signaling pathways involved in the regulation of proliferation or apoptosis. A comparison of piRNA expression in tumor tissue and adjacent healthy tissues has demonstrated they can be used as biomarkers. However, this way of obtaining samples has a significant drawback, which is the invasiveness of such a procedure. Liquid biopsy is an alternative for acquiring biological material with little to no harm to a patient. Several different piRNAs in various types of cancer were shown to be expressed in bodily fluids such as blood or urine. Furthermore, their expression significantly differed between cancer patients and healthy individuals. Hence, this review aimed to assess the possible use of liquid biopsy for cancer diagnosis with piRNAs as biomarkers. Full article
(This article belongs to the Special Issue The Role of Non-coding RNAs (ncRNAs) in Molecular Diagnosis of Diseases)
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