New insides in Fluorescent and Colorimetric Probes

A special issue of Chemosensors (ISSN 2227-9040).

Deadline for manuscript submissions: closed (31 January 2021) | Viewed by 14399

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BIOSCOPE Group, LAQV@REQUIMTE, Chemistry Department, Faculty of Science and Technology, NOVA University Lisbon, Caparica, Portugal
Interests: antibiotic resistance; MALDI-TOF mass spectrometry; analytical proteomics; biomarker discovery; analytical chemistry; biochemistry
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Dear Colleagues,

Light and colors are important analytical tools, useful for the detection and quantification of different analytes, since the beginning of times. In fact, fluorescence was already used by the first time for the determination of numerous and diverse organic and/or inorganic components with great sensitivity and selectivity.

Nowadays, many sensing-based emissive and colorimentric materials are effectual methods for the detection of trace and ultra-trace amounts of contaminants and inorganic/organic components in complicated matrices.

The guest editors cordially encourage you to submitted your research paper devoted to “Fluorescent and Colorimetric Chemosensors” and to contribute to this Special Issue devoted to this fascinating research topic, discussed at the BIOSCOPE Caparica Conference Series #IC3EM; #PTIM; #IC3TC.

Prof. Dr. Carlos Lodeiro
Prof. Dr. José Luis Capelo
Dr. Elisabete Oliveira
Dr. Javier Fernández-Lodeiro
Guest Editors

Manuscript Submission Information

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Keywords

  • Applications of fluorescent and colorimetric chemosensors in microscopy
  • Cell and tissue Imaging
  • Emissive probes, and labels for organic and inorganic analytes
  • Detection and imaging in Biology/Medicine/Biochemistry
  • Smart emissive liquids crystals and polymers
  • Functionalized nanomaterials for sensing
  • Chemosensors in Art
  • Environmental applications of Chemosensors

Published Papers (3 papers)

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Research

10 pages, 2527 KiB  
Article
Flavylium Dye as pH-Tunable Fluorescent and CD Probe for Double-Stranded DNA and RNA
by Ivo Crnolatac, Letícia Giestas, Gordan Horvat, António Jorge Parola and Ivo Piantanida
Chemosensors 2020, 8(4), 129; https://doi.org/10.3390/chemosensors8040129 - 13 Dec 2020
Cited by 5 | Viewed by 2570
Abstract
The interaction of 4′-(N,N-dimethylamino)-6-hydroxyflavylium cation with double stranded (ds-) DNA/RNA was studied by UV/Vis spectrophotometry, circular dichroism (CD), and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic [...] Read more.
The interaction of 4′-(N,N-dimethylamino)-6-hydroxyflavylium cation with double stranded (ds-) DNA/RNA was studied by UV/Vis spectrophotometry, circular dichroism (CD), and also steady-state and time-resolved emission spectroscopies at neutral and weakly acidic conditions. At pH 5, the studied molecule, in its flavylium cationic form, showed considerable binding affinities (5 < logKs < 6) for all ds-DNA/RNA, contrary to chalcones forms (dominant at pH 7), which did not show binding to polynucleotides. Flavylium cation intercalated into ds-DNAs at variance to dominant groove aggregation within ds-RNA, which was reported by RNA-specific bisignate induced CD spectrum (ICD) bands. The intrinsically negligible fluorescence of flavylium was strongly increased upon the addition of DNA or RNA, whereby both the fluorescence intensity and emission lifetimes of complexes differed considerably: the strongest emission increase was observed for AU-RNA (detection limit estimated to 10 nM) followed by AT-DNAs and the much weaker effect of GC-DNAs. Both fluorescence sensitivity on the ds-DNA/RNA secondary structure and sequence-selective ICD bands make the flavylium–chalcones system an intriguing pH-switchable new probe for distinguishing between various polynucleotide sequences. Full article
(This article belongs to the Special Issue New insides in Fluorescent and Colorimetric Probes)
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12 pages, 2985 KiB  
Article
Surface Functionalization of Cotton Fabric with Fluorescent Dendrimers, Spectral Characterization, Cytotoxicity, Antimicrobial and Antitumor Activity
by Ivo Grabchev, Desislava Staneva, Evgenia Vasileva-Tonkova and Radostina Alexandrova
Chemosensors 2019, 7(2), 17; https://doi.org/10.3390/chemosensors7020017 - 30 Mar 2019
Cited by 16 | Viewed by 4139
Abstract
Poly(propylenimine) dendrimers from first and third generations modified with 1,8-naphthalimide units and their Zn(II) complexes have been investigated by absorption and fluorescence spectroscopy. These dendrimers have been deposited on a cotton cloth by the extraction method, producing yellow-colored textile materials. They have been [...] Read more.
Poly(propylenimine) dendrimers from first and third generations modified with 1,8-naphthalimide units and their Zn(II) complexes have been investigated by absorption and fluorescence spectroscopy. These dendrimers have been deposited on a cotton cloth by the extraction method, producing yellow-colored textile materials. They have been characterized by defining their color coordinates L*a*b*, XYZ and xy. The antimicrobial activity of dendrimers has been investigated in vitro against model gram-positive and gram-negative bacteria and yeasts. Being deposited onto the surface of cotton fabric, the studied dendrimers reduced bacterial growth and prevented the formation of bacterial biofilm. Anticancer and cytotoxicity activities have also been performed against HeLa and Lep-3 human tumor cell lines as model systems. Full article
(This article belongs to the Special Issue New insides in Fluorescent and Colorimetric Probes)
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11 pages, 2646 KiB  
Article
Lifetime and Fluorescence Quantum Yield of Two Fluorescein-Amino Acid-Based Compounds in Different Organic Solvents and Gold Colloidal Suspensions
by Viviane Pilla, Augusto C. Gonçalves, Alcindo A. Dos Santos and Carlos Lodeiro
Chemosensors 2018, 6(3), 26; https://doi.org/10.3390/chemosensors6030026 - 30 Jun 2018
Cited by 10 | Viewed by 6812
Abstract
Fluorescein and its derivatives are yellowish-green emitting dyes with outstanding potential in advanced molecular probes for different applications. In this work, two fluorescent compounds containing fluorescein and an amino acid residue (compounds 1 and 2) were photophysically explored. Compounds 1 and 2 [...] Read more.
Fluorescein and its derivatives are yellowish-green emitting dyes with outstanding potential in advanced molecular probes for different applications. In this work, two fluorescent compounds containing fluorescein and an amino acid residue (compounds 1 and 2) were photophysically explored. Compounds 1 and 2 were previously synthesized via ester linkage between fluorescein ethyl ester and Boc-ser (TMS)-OH or Boc-cys(4-methyl benzyl)-OH. Studies on the time-resolved fluorescence lifetime and relative fluorescence quantum yield (φ) were performed for 1 and 2 diluted in acetonitrile, ethanol, dimethyl sulfoxide, and tetrahydrofuran solvents. The discussion considered the dielectric constants of the studied solvents (range between 7.5 and 47.2) and the photophysical parameters. The results of the lifetime and φ were compared with those obtained for compounds 1, 2 and fluorescein without an amino acid residue in alkaline aqueous solutions. Moreover, as a preliminary result compound 2 (S-cysteine) was tested in the presence of gold nanoparticles as an aggregation colorimetric probe. Full article
(This article belongs to the Special Issue New insides in Fluorescent and Colorimetric Probes)
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