Research on Chlamydomonas Cell Biology

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Plant, Algae and Fungi Cell Biology".

Deadline for manuscript submissions: closed (15 December 2021) | Viewed by 3483

Special Issue Editors

К.А. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., 12 7276 Moscow, Russia
Interests: photosynthesis; plant physiology; environmental stress; abiotic stress; UV radiation; photoreceptor signalling; cyanobacteria; algal; ROS; nonphotochemical quenching (NPQ); chlorophyll fluorescence; salt stress; hydrogen energy; artificial photosynthesis
Special Issues, Collections and Topics in MDPI journals
Department of Plant Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, India
Interests: Chloroplast Bioenergestics; Abiotic Stress; Omics; Light signalling

Special Issue Information

Dear Colleagues,

The unicellular haploid green alga Chlamydomonas reinhardtii is a powerful model organism for investigating basic biological processes in photosynthetic eukaryotes, including chloroplast biogenesis, photosynthesis, motility, light perception, stress biology and cell cycle control. In addition, this organism is used extensively for bioresource technology. The availability of an annotated nuclear genome and a growing array of tools and techniques for molecular genetic studies have boosted research on fundamental questions in the cell and molecular biology of this model organism. For this Special Issue of Cells, we request your contributions in the form of reviews, original research articles or shorter “perspective” articles on all aspects related to Chlamydomonas cell biology, such as plastid biogenesis, cell signaling, biofuel production, carbon metabolism, nutrient assimilation, stress biology, autophagy, flagella biology, organelle structure or vacuole function.

Prof. Dr. Suleyman Allakhverdiev
Prof. Dr. Rajagopal Subramanyam
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chlamydomonas
  • chloroplast biogenesis
  • photosynthesis
  • carbon source, cell signaling
  • light perception
  • cell cycle
  • autophagy
  • bioresource
  • mutagenesis
  • omics
  • abiotic and biotic stress

Published Papers (1 paper)

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Research

14 pages, 2836 KiB  
Article
Microfluidic Platforms Designed for Morphological and Photosynthetic Investigations of Chlamydomonas reinhardtii on a Single-Cell Level
by Eszter Széles, Krisztina Nagy, Ágnes Ábrahám, Sándor Kovács, Anna Podmaniczki, Valéria Nagy, László Kovács, Péter Galajda and Szilvia Z. Tóth
Cells 2022, 11(2), 285; https://doi.org/10.3390/cells11020285 - 14 Jan 2022
Cited by 6 | Viewed by 2852
Abstract
Chlamydomonas reinhardtii is a model organism of increasing biotechnological importance, yet, the evaluation of its life cycle processes and photosynthesis on a single-cell level is largely unresolved. To facilitate the study of the relationship between morphology and photochemistry, we established microfluidics in combination [...] Read more.
Chlamydomonas reinhardtii is a model organism of increasing biotechnological importance, yet, the evaluation of its life cycle processes and photosynthesis on a single-cell level is largely unresolved. To facilitate the study of the relationship between morphology and photochemistry, we established microfluidics in combination with chlorophyll a fluorescence induction measurements. We developed two types of microfluidic platforms for single-cell investigations: (i) The traps of the “Tulip” device are suitable for capturing and immobilizing single cells, enabling the assessment of their photosynthesis for several hours without binding to a solid support surface. Using this “Tulip” platform, we performed high-quality non-photochemical quenching measurements and confirmed our earlier results on bulk cultures that non-photochemical quenching is higher in ascorbate-deficient mutants (Crvtc2-1) than in the wild-type. (ii) The traps of the “Pot” device were designed for capturing single cells and allowing the growth of the daughter cells within the traps. Using our most performant “Pot” device, we could demonstrate that the FV/FM parameter, an indicator of photosynthetic efficiency, varies considerably during the cell cycle. Our microfluidic devices, therefore, represent versatile platforms for the simultaneous morphological and photosynthetic investigations of C. reinhardtii on a single-cell level. Full article
(This article belongs to the Special Issue Research on Chlamydomonas Cell Biology)
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