Effects of Hypoxic Preconditioning in Mesenchymal Stem Cells

A special issue of Cells (ISSN 2073-4409). This special issue belongs to the section "Stem Cells".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 2629

Special Issue Editors


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Special Issue Information

Dear Colleagues,

Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate in a wide variety of tissues in order to participate in tissue regeneration at local sites after injury or disease. MSCs have been found in different tissues and have emerged as a promising tool for cell-based therapy. They can be isolated from bone marrow, adipose tissue, oral cavity, umbilical cord blood, periosteum and other parts of the body. Several studies have demonstrated the effects of hypoxia on MSCs, including proliferation, differentiation, survival rate, cytokine or growth factor secretions and cell biosafety.

MSCs in their microenvironment can react to different cues, such as inflammation and hypoxia. Hypoxia is defined as an oxygen tension that is lower than physoxia (i.e., 3–9% oxygen in most tissues). The culturing of MSCs with low oxygen tension has recently attracted significant research interest. Several studies are necessary to demonstrate the potential beneficial effects of hypoxia on MSCs derived from a wide range of sources. To better elucidate the therapeutic benefits of hypoxic preconditioning, further investigations are still required to evaluate the cellular mechanisms triggered by hypoxia treatment. Hypoxic preconditioning could have an effective role in wound healing, cardiomyocyte regeneration, and bone and cartilage repair, indicating their great potential therapeutic application in ischemic diseases and skeletal tissue defects.

Prof. Dr. Oriana Trubiani
Dr. Francesca Diomede
Dr. Jacopo Pizzicanella
Guest Editors

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Keywords

  • hypoxia
  • mesenchymal stem cells
  • stem-cell-based therapy
  • cell differentiation
  • regenerative medicine

Published Papers (1 paper)

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19 pages, 3058 KiB  
Article
The Journey of SCAPs (Stem Cells from Apical Papilla), from Their Native Tissue to Grafting: Impact of Oxygen Concentration
by Marine Mavinga, Mathilde Palmier, Murielle Rémy, Caroline Jeannière, Solène Lenoir, Sylvie Rey, Martine Saint-Marc, Florian Alonso, Elisabeth Génot, Noélie Thébaud, Edith Chevret, Virginie Mournetas, Benoit Rousseau, Claudine Boiziau and Helene Boeuf
Cells 2022, 11(24), 4098; https://doi.org/10.3390/cells11244098 - 16 Dec 2022
Cited by 4 | Viewed by 2254
Abstract
Tissue engineering strategies aim at characterizing and at optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present the immunoMap of apical papilla, the native tissue from which SCAPs are derived. We characterized stem cell niches that [...] Read more.
Tissue engineering strategies aim at characterizing and at optimizing the cellular component that is combined with biomaterials, for improved tissue regeneration. Here, we present the immunoMap of apical papilla, the native tissue from which SCAPs are derived. We characterized stem cell niches that correspond to a minority population of cells expressing Mesenchymal stromal/Stem Cell (CD90, CD105, CD146) and stemness (SSEA4 and CD49f) markers as well as endothelial cell markers (VWF, CD31). Based on the colocalization of TKS5 and cortactin markers, we detected migration-associated organelles, podosomes-like structures, in specific regions and, for the first time, in association with stem cell niches in normal tissue. From six healthy teenager volunteers, each with two teeth, we derived twelve cell banks, isolated and amplified under 21 or 3% O2. We confirmed a proliferative advantage of all banks when cultured under 3% versus 21% O2. Interestingly, telomerase activity was similar to that of the highly proliferative hiPSC cell line, but unrelated to O2 concentration. Finally, SCAPs embedded in a thixotropic hydrogel and implanted subcutaneously in immunodeficient mice were protected from cell death with a slightly greater advantage for cells preconditioned at 3% O2. Full article
(This article belongs to the Special Issue Effects of Hypoxic Preconditioning in Mesenchymal Stem Cells)
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