New Technologies in Agricultural and Plant Biotechnology

A special issue of BioTech (ISSN 2673-6284). This special issue belongs to the section "Agricultural and Food Biotechnology".

Deadline for manuscript submissions: closed (1 September 2023) | Viewed by 11629

Special Issue Editors


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Guest Editor
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 11884 Nasr City, Cairo, Egypt
Interests: nanomaterials; bioinformatics; plant disease resistance; medicinal plants; biotechnology

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Guest Editor
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, 11884 Nasr City, Cairo, Egypt
Interests: plant physiology; symbiotic relationships in plants; enzymology; bioinformatics; tissue culture
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Special Issue Information

Dear Colleagues,

Nanotechnology has recently been used as a tool in industries including agriculture, industry, and medicine. These composites have improved the plant pathogen defense, drought resistance, salt resistance, and heavy-metal resistance in agricultural fields. In addition, nanotechnology was employed to make herbicides and insecticides more effective, enabling the use of lower dosages while lowering pollution and cleaning up already-existing contaminants. Numerous agricultural uses, such as tissue culture and antibacterial compounds, depend on nanotechnology. In addition to the scientific novelty, technologies in which new tools sometimes reflect fundamentally new methods of genome alteration offer lawmakers a fresh issue by providing genetically modified creatures new meanings in both the biochemical/molecular and legal senses (GMOs). Although all of these techniques use biotechnology to develop new plant types, it is debatable whether they all produce GMOs as defined by the law at the moment. The responsibility of remarkable weight for the authority is the official risk evaluation of these technologies.

Prof. Dr. Ahmed R. Sofy
Prof. Dr. Mahmoud R. Sofy
Guest Editors

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Keywords

  • biotechnology
  • tissue culture
  • plant disease
  • biotic and abiotic stress
  • bioinformatics

Published Papers (3 papers)

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Research

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14 pages, 2881 KiB  
Article
Application of Chlorella vulgaris Beijerinck as a Biostimulant for Growing Cucumber Seedlings in Hydroponics
by Galiya I. Vildanova, Rezeda Z. Allaguvatova, Dina F. Kunsbaeva, Natalia V. Sukhanova and Lira A. Gaysina
BioTech 2023, 12(2), 42; https://doi.org/10.3390/biotech12020042 - 22 May 2023
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Abstract
Hydroponics is a promising method for growing agricultural plants and is especially relevant in the context of global climate change. Microscopic algae, including Chlorella vulgaris, have great potential for use in hydroponic systems as natural growth stimulators. The effect of the suspension [...] Read more.
Hydroponics is a promising method for growing agricultural plants and is especially relevant in the context of global climate change. Microscopic algae, including Chlorella vulgaris, have great potential for use in hydroponic systems as natural growth stimulators. The effect of the suspension of an authentic strain of Chlorella vulgaris Beijerinck on the length of cucumber shoots and roots, as well as its dry biomass, was studied. During cultivation in a Knop medium with the addition of Chlorella suspension, the length of the shoots was shortened from 11.30 to 8.15 cm, while the length of the roots also decreased from 16.41 to 10.59 cm. At the same time, the biomass of the roots increased from 0.04 to 0.05 g. The data obtained indicate the positive effect of the suspension of the Chlorella vulgaris authentic strain on the dry biomass of cucumber plants in hydroponic conditions and make it possible to recommend this strain for use when growing plants in hydroponic systems. Full article
(This article belongs to the Special Issue New Technologies in Agricultural and Plant Biotechnology)
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14 pages, 1047 KiB  
Article
Molecular and Physiological Variability in Bread Wheat and Its Wild Relative (Aegilops tauschii Coss.) Species under Water-Deficit Stress Conditions
by Zahra Khodadadi, Mansoor Omidi, Alireza Etminan, Asa Ebrahimi and Alireza Pour-Aboughadareh
BioTech 2023, 12(1), 3; https://doi.org/10.3390/biotech12010003 - 28 Dec 2022
Cited by 1 | Viewed by 1915
Abstract
Aegilops and Triticum spp. are two ideal gene pools for the breeding purposes of wheat. In this study, a set of Iranian accessions of Aegilops tauschii Coss. and Triticum aestivum L. species were evaluated in terms of some physiological and biochemical features under [...] Read more.
Aegilops and Triticum spp. are two ideal gene pools for the breeding purposes of wheat. In this study, a set of Iranian accessions of Aegilops tauschii Coss. and Triticum aestivum L. species were evaluated in terms of some physiological and biochemical features under control and water-deficit stress conditions. Moreover, several simple sequence repeat (SSR) markers were employed to identify marker loci associated with the measured traits. The results indicated that water-deficit stress significantly affected all measured traits and the highest reductions due to water-deficit were recorded for shoot fresh and dry biomasses (SFB and SDB), stomatal conductance (Gs), leaf relative water content (RWC), and chlorophyll b content (Chl b). In molecular analysis, 25 SSR markers generated 50 fragments, out of which 49 fragments (98%) were polymorphic. Furthermore, the genetic variation observed within species is more than between species. The results of cluster and Bayesian model analysis classified all evaluated accessions into three main clusters. Under control and water-deficit stress conditions, 28 and 27 significant marker-trait associations (MTAs) were identified, respectively. Furthermore, 10 MTAs showed sufficiently stable expression across both growth conditions. Of these, the markers Xgwm-111, Xgwm-44, Xgwm-455, Xgwm-272, and Xgwm-292 were associated with multiple traits. Hence, these markers could serve as useful molecular tools for population characterization, gene tagging, and other molecular breeding studies. Full article
(This article belongs to the Special Issue New Technologies in Agricultural and Plant Biotechnology)
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Review

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22 pages, 1340 KiB  
Review
Biotechnological Interventions in Tomato (Solanum lycopersicum) for Drought Stress Tolerance: Achievements and Future Prospects
by Ram Krishna, Waquar Akhter Ansari, P. S. Soumia, Akhilesh Yadav, Durgesh Kumar Jaiswal, Sudhir Kumar, Achuit Kumar Singh, Major Singh and Jay Prakash Verma
BioTech 2022, 11(4), 48; https://doi.org/10.3390/biotech11040048 - 19 Oct 2022
Cited by 11 | Viewed by 5917
Abstract
Tomato production is severely affected by abiotic stresses (drought, flood, heat, and salt) and causes approximately 70% loss in yield depending on severity and duration of the stress. Drought is the most destructive abiotic stress and tomato is very sensitive to the drought [...] Read more.
Tomato production is severely affected by abiotic stresses (drought, flood, heat, and salt) and causes approximately 70% loss in yield depending on severity and duration of the stress. Drought is the most destructive abiotic stress and tomato is very sensitive to the drought stress, as cultivated tomato lack novel gene(s) for drought stress tolerance. Only 20% of agricultural land worldwide is irrigated, and only 14.51% of that is well-irrigated, while the rest is rain fed. This scenario makes drought very frequent, which restricts the genetically predetermined yield. Primarily, drought disturbs tomato plant physiology by altering plant–water relation and reactive oxygen species (ROS) generation. Many wild tomato species have drought tolerance gene(s); however, their exploitation is very difficult because of high genetic distance and pre- and post-transcriptional barriers for embryo development. To overcome these issues, biotechnological methods, including transgenic technology and CRISPR-Cas, are used to enhance drought tolerance in tomato. Transgenic technology permitted the exploitation of non-host gene/s. On the other hand, CRISPR-Cas9 technology facilitated the editing of host tomato gene(s) for drought stress tolerance. The present review provides updated information on biotechnological intervention in tomato for drought stress management and sustainable agriculture. Full article
(This article belongs to the Special Issue New Technologies in Agricultural and Plant Biotechnology)
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