Novel Sensing System for Biomedical Applications

A topical collection in Biosensors (ISSN 2079-6374).

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Editors


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Collection Editor
Department of Biological Science and Technology, National Chiao Tung University, 75 Bo-Ai Street, Hsinchu 30068, Taiwan
Interests: biointerface science and technology; biomaterials; biophotonics; biosensors; bioelectronics; soft condensed matter; biological physics

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Collection Editor
National Chiao Tung University, 75 Bo-Ai street, Hsinchu 30068, Taiwan
Interests: electrochemistry; interfacial technology; biosensors and biosensing systems; biomimetic and nanobiomedical material

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Collection Editor
National Cheng Kung University, No.1, University Road, Tainan City 701, Taiwan
Interests: optical nanomaterials; SERS biodiagnostic platform; biomedical imaging; optical analysis-guided PDT/PTT; green biosensors

Topical Collection Information

Dear Colleague,

Diagnostics is important to discover, verify, and justify the occurrence, progression, and recovery from human diseases. It is also being increasingly adopted for the guidance of patient management from diagnosis to treatment in many human diseases, such as cardiovascular diseases, cancers, infectious diseases, congenital abnormalities, and much more. For example, from last winter to date, the emerging pathogen, i.e., SARS-CoV-2, spread rapidly across the world. Sensitive, accurate, rapid, and cost-effective sensing methods are being highly demanded to prevent the further spreading of virus or pathogens. The increasing need for information and to understand the mechanisms underlying disease development have led to rapid expansion in the development of biomarkers and sensing technologies in clinics and laboratories. Biosensing technology has been proven to play pivotal roles in a variety of biomedical applications and fuel the growth of telemedicine, e-Health, and e-Hospitals. Modern biosensors also exhibit great potential to be integrated into electronic healthcare monitoring systems to increase healthcare workforce scarcity.

The aims and scope of this Special Issue of Biosensors will focus on themes captured in the title, Novel Sensing Systems for Biomedical Applications. This issue is intended to cover but not limited to the aspects of modern development of biosensing technologies, such as photoacoustic imaging (PAI), optical coherence tomography (OCT), fluorescent diagnosis and Raman spectroscopy, interfacial technologies, electronic devices, point-of-care devices and systems and their biomedical applications in laboratories or in clinics, etc. However, there are still many challenges to applying these analysis techniques to clinical or even point-of-care testing. Therefore, we strongly encourage the submission of articles on biosensing applications in biomedical analytical sensors research to promote the development of early diagnosis and precision medicine.

Prof. Dr. Chia-Ching Chang
Prof. Dr. Chiun-Jye Yuan
Prof. Chih-Chia Huang
Collection Editors

Manuscript Submission Information

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Keywords

  • Biomarkers sensing
  • Bioimaging
  • Antibody for virus detection
  • Aptamer for protein or biomolecules detection
  • Novel biosensing instrumental developments
  • Biosensing for novel diagnosis method
  • Flexible, printable, and biocompatible sensors and systems
  • New optical analysis technology
  • Nanoparticle-enhanced selectivity and sensitivity
  • Nanoparticles-assisted signal enrichment system
  • Biosensor for real-time monitoring
  • Single molecular detection
  • Biosensor for point-of-care analysis

Published Papers (7 papers)

2021

Jump to: 2020

12 pages, 2153 KiB  
Article
Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System
by Hoibin Jeong, Song-Rae Kim, Yujung Kang, Huisu Kim, Seo-Young Kim, Su-Hyeon Cho and Kil-Nam Kim
Biosensors 2021, 11(12), 471; https://doi.org/10.3390/bios11120471 - 23 Nov 2021
Cited by 2 | Viewed by 2215
Abstract
Tumor angiogenesis is enhanced in all types of tumors to supply oxygen and nutrients for their growth and metastasis. With the development of anti-angiogenic drugs, the importance of technology that closely monitors tumor angiogenesis has also been emerging. However, to date, the technology [...] Read more.
Tumor angiogenesis is enhanced in all types of tumors to supply oxygen and nutrients for their growth and metastasis. With the development of anti-angiogenic drugs, the importance of technology that closely monitors tumor angiogenesis has also been emerging. However, to date, the technology for observing blood vessels requires specialized skills with expensive equipment, thereby limiting its applicability only to the laboratory setting. Here, we used a preclinical optical imaging system for small animals and, for the first time, observed, in real time, the entire process of blood vessel development in tumor-bearing mice injected with indocyanine green. Time-lapse sequential imaging revealed blood vessel volume and blood flow dynamics on a microscopic scale. Upon analyzing fluorescence dynamics at each stage of tumor progression, vessel volume and blood flow were found to increase as the tumor developed. Conversely, these vascular parameters decreased when the mice were treated with angiogenesis inhibitors, which suggests that the effects of drugs targeting angiogenesis can be rapidly and easily screened. The results of this study may help evaluate the efficacy of angiogenesis-targeting drugs by facilitating the observation of tumor blood vessels easily in a laboratory unit without large and complex equipment. Full article
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14 pages, 3083 KiB  
Article
Accuracy of the Electronic Nose Breath Tests in Clinical Application: A Systematic Review and Meta-Analysis
by Hsiao-Yu Yang, Wan-Chin Chen and Rodger-Chen Tsai
Biosensors 2021, 11(11), 469; https://doi.org/10.3390/bios11110469 - 22 Nov 2021
Cited by 8 | Viewed by 2702
Abstract
(1) Background: An electronic nose applies a sensor array to detect volatile biomarkers in exhaled breath to diagnose diseases. The overall diagnostic accuracy remains unknown. The objective of this review was to provide an estimate of the diagnostic accuracy of sensor-based breath tests [...] Read more.
(1) Background: An electronic nose applies a sensor array to detect volatile biomarkers in exhaled breath to diagnose diseases. The overall diagnostic accuracy remains unknown. The objective of this review was to provide an estimate of the diagnostic accuracy of sensor-based breath tests for the diagnosis of diseases. (2) Methods: We searched the PubMed and Web of Science databases for studies published between 1 January 2010 and 14 October 2021. The search was limited to human studies published in the English language. Clinical trials were not included in this review. (3) Results: Of the 2418 records identified, 44 publications were eligible, and 5728 patients were included in the final analyses. The pooled sensitivity was 90.0% (95% CI, 86.3–92.8%, I2 = 47.7%), the specificity was 88.4% (95% CI, 87.1–89.5%, I2 = 81.4%), and the pooled area under the curve was 0.93 (95% CI 0.91–0.95). (4) Conclusion: The findings of our review suggest that a standardized report of diagnostic accuracy and a report of the accuracy in a test set are needed. Sensor array systems of electronic noses have the potential for noninvasiveness at the point-of-care in hospitals. Nevertheless, the procedure for reporting the accuracy of a diagnostic test must be standardized. Full article
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14 pages, 3153 KiB  
Article
A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection
by Ping Ouyang, Chenxin Fang, Jialun Han, Jingjing Zhang, Yuxing Yang, Yang Qing, Yubing Chen, Wenyan Shang and Jie Du
Biosensors 2021, 11(11), 451; https://doi.org/10.3390/bios11110451 - 13 Nov 2021
Cited by 3 | Viewed by 2416
Abstract
The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy [...] Read more.
The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal protecting effects of small-molecule-linked DNA. This strategy is demonstrated using the small molecule biotin and its receptor protein streptavidin (SA). We immobilized DNA with a designed structure and sequence on the surface of the gold electrode, and we named it M1-Biotin DNA. M1-Biotin DNA selectively combines with SA to generate M1-Biotin-SA DNA and protects M1-Biotin DNA from digestion by EXO III; therefore, M1-Biotin DNA remains intact on the electrode surface. M1-Biotin-SA DNA was modified with methylene blue (MB); the MB reporter molecule is located near the surface of the gold electrode, which generates a substantial electrochemical signal during the detection of SA. Through this strategy, we can exploit the presence or absence of an electrochemical signal to provide qualitative target protein determination as well as the strength of the electrochemical signal to quantitatively analyze the target protein concentration. This strategy has been proven to be used for the quantitative analysis of the interaction between biotin and streptavidin (SA). Under optimal conditions, the detection limit of the proposed biosensor is as low as 18.8 pM, and the linear range is from 0.5 nM to 5 μM, showing high sensitivity. The detection ability of this DNA biosensor in complex serum samples has also been studied. At the same time, we detected the folate receptor (FR) to confirm that this strategy can be used to detect other proteins. Therefore, this electrochemical DNA biosensor provides a sensitive, low-cost, and fast target protein detection platform, which may provide a reliable and powerful tool for early disease diagnosis. Full article
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11 pages, 25787 KiB  
Article
Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
by Tania Pomili, Paolo Donati and Pier Paolo Pompa
Biosensors 2021, 11(11), 443; https://doi.org/10.3390/bios11110443 - 10 Nov 2021
Cited by 18 | Viewed by 3166
Abstract
In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing [...] Read more.
In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing a pre-treatment and test zone. Its one-step fabrication is realized by CO2 laser cutting, providing remarkable parallelization and rapidity (ca. 5 s/device). The colorimetric detection is based on the sensitive and selective target-induced reshaping of plasmonic multibranched gold nanoparticles, which exhibit a clear spectral shift (and blue-to-pink color change) in case of non-physiological concentrations of the three salivary biomarkers. A rapid and multiplexed naked-eye or smartphone-based readout of the colorimetric response is achieved within 10 min. A prototype kit for POCT testing is also reported, providing robustness and easy handling of the device. Full article
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26 pages, 5558 KiB  
Review
Recent Advances in Novel Lateral Flow Technologies for Detection of COVID-19
by Wesley Wei-Wen Hsiao, Trong-Nghia Le, Dinh Minh Pham, Hui-Hsin Ko, Huan-Cheng Chang, Cheng-Chung Lee, Neha Sharma, Cheng-Kang Lee and Wei-Hung Chiang
Biosensors 2021, 11(9), 295; https://doi.org/10.3390/bios11090295 - 25 Aug 2021
Cited by 60 | Viewed by 16025
Abstract
The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as [...] Read more.
The development of reliable and robust diagnostic tests is one of the most efficient methods to limit the spread of coronavirus disease 2019 (COVID-19), which is caused by the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). However, most laboratory diagnostics for COVID-19, such as enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase-polymerase chain reaction (RT-PCR), are expensive, time-consuming, and require highly trained professional operators. On the other hand, the lateral flow immunoassay (LFIA) is a simpler, cheaper device that can be operated by unskilled personnel easily. Unfortunately, the current technique has some limitations, mainly inaccuracy in detection. This review article aims to highlight recent advances in novel lateral flow technologies for detecting SARS-CoV-2 as well as innovative approaches to achieve highly sensitive and specific point-of-care testing. Lastly, we discuss future perspectives on how smartphones and Artificial Intelligence (AI) can be integrated to revolutionize disease detection as well as disease control and surveillance. Full article
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11 pages, 1665 KiB  
Article
Development of the Sensing Platform for Protein Tyrosine Kinase Activity
by Lan-Yi Wei, Wei Lin, Bey-Fen Leo, Lik-Voon Kiew, Chia-Ching Chang and Chiun-Jye Yuan
Biosensors 2021, 11(7), 240; https://doi.org/10.3390/bios11070240 - 15 Jul 2021
Viewed by 2177
Abstract
A miniature tyrosinase-based electrochemical sensing platform for label-free detection of protein tyrosine kinase activity was developed in this study. The developed miniature sensing platform can detect the substrate peptides for tyrosine kinases, such as c-Src, Hck and Her2, in a low sample volume [...] Read more.
A miniature tyrosinase-based electrochemical sensing platform for label-free detection of protein tyrosine kinase activity was developed in this study. The developed miniature sensing platform can detect the substrate peptides for tyrosine kinases, such as c-Src, Hck and Her2, in a low sample volume (1–2 μL). The developed sensing platform exhibited a high reproducibility for repetitive measurement with an RSD (relative standard deviation) of 6.6%. The developed sensing platform can detect the Hck and Her2 in a linear range of 1–200 U/mL with the detection limit of 1 U/mL. The sensing platform was also effective in assessing the specificity and efficacies of the inhibitors for protein tyrosine kinases. This is demonstrated by the detection of significant inhibition of Hck (~88.1%, but not Her2) by the Src inhibitor 1, an inhibitor for Src family kinases, as well as the significant inhibition of Her2 (~91%, but not Hck) by CP-724714 through the platform. These results suggest the potential of the developed miniature sensing platform as an effective tool for detecting different protein tyrosine kinase activity and for accessing the inhibitory effect of various inhibitors to these kinases. Full article
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2020

Jump to: 2021

19 pages, 1326 KiB  
Review
Universal Glia to Neurone Lactate Transfer in the Nervous System: Physiological Functions and Pathological Consequences
by Carolyn L. Powell, Anna R. Davidson and Angus M. Brown
Biosensors 2020, 10(11), 183; https://doi.org/10.3390/bios10110183 - 19 Nov 2020
Cited by 18 | Viewed by 3468
Abstract
Whilst it is universally accepted that the energy support of the brain is glucose, the form in which the glucose is taken up by neurones is the topic of intense debate. In the last few decades, the concept of lactate shuttling between glial [...] Read more.
Whilst it is universally accepted that the energy support of the brain is glucose, the form in which the glucose is taken up by neurones is the topic of intense debate. In the last few decades, the concept of lactate shuttling between glial elements and neural elements has emerged in which the glial cells glycolytically metabolise glucose/glycogen to lactate, which is shuttled to the neural elements via the extracellular fluid. The process occurs during periods of compromised glucose availability where glycogen stored in astrocytes provides lactate to the neurones, and is an integral part of the formation of learning and memory where the energy intensive process of learning requires neuronal lactate uptake provided by astrocytes. More recently sleep, myelination and motor end plate integrity have been shown to involve lactate shuttling. The sequential aspect of lactate production in the astrocyte followed by transport to the neurones is vulnerable to interruption and it is reported that such disparate pathological conditions as Alzheimer’s disease, amyotrophic lateral sclerosis, depression and schizophrenia show disrupted lactate signalling between glial cells and neurones. Full article
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