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Biosensors
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Novel Biosensors for Cell Analysis
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Novel Biosensors for Cell Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 10419

Special Issue Editor

Dr. Chongli Yuan

E-Mail Website
Guest Editor
1. Davidson School of Chemical Engineering, Purdue University, IN 47906, USA
2. Purdue University Center for Cancer Research, Purdue University, IN 47906, USA
Interests: quantitative single-cell analysis; FRET; BiFC; live cell tracking; chromatin dynamics; cell signaling dynamics

Special Issue Information

Dear Colleagues,

Cellular events such as changes in chromatin during cell replication, neurotransmitter uptakes, and the formation of signaling complexes occur at vastly different time and length scales inside a cell. Tracking these dynamic events is crucial for understanding fundamental biological mechanisms and providing useful insights for engineering novel cell types and/or devising cellular therapy. Recent advances in synthetic biology have enabled the development of an array of biosensors to track cellular events. This Issue will focus on the latest developments in biosensors to track in situ cellular behavior enabling single-cell-based analysis to reveal cellular heterogeneity and dynamics.

Dr. Chongli Yuan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • quantitative single-cell analysis
  • FRET
  • BiFC
  • live cell tracking
  • chromatin dynamics
  • cell signaling dynamics

Published Papers (5 papers)

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Research

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14 pages, 6259 KiB  
Article
Enhancing the Study of Quantal Exocytotic Events: Combining Diamond Multi-Electrode Arrays with Amperometric PEak Analysis (APE) an Automated Analysis Code
by Giulia Tomagra, Alice Re, Veronica Varzi, Pietro Aprà, Adam Britel, Claudio Franchino, Sofia Sturari, Nour-Hanne Amine, Remco H. S. Westerink, Valentina Carabelli and Federico Picollo
Biosensors 2023, 13(12), 1033; https://doi.org/10.3390/bios13121033 - 16 Dec 2023
Viewed by 1385
Abstract
MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them [...] Read more.
MicroGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) can be successfully used to reveal, in real time, quantal exocytotic events occurring from many individual neurosecretory cells and/or from many neurons within a network. As μG-D-MEAs arrays are patterned with up to 16 sensing microelectrodes, each of them recording large amounts of data revealing the exocytotic activity, the aim of this work was to support an adequate analysis code to speed up the signal detection. The cutting-edge technology of microGraphited-Diamond-Multi Electrode Arrays (μG-D-MEAs) has been implemented with an automated analysis code (APE, Amperometric Peak Analysis) developed using Matlab R2022a software to provide easy and accurate detection of amperometric spike parameters, including the analysis of the pre-spike foot that sometimes precedes the complete fusion pore dilatation. Data have been acquired from cultured PC12 cells, either collecting events during spontaneous exocytosis or after L-DOPA incubation. Validation of the APE code was performed by comparing the acquired spike parameters with those obtained using Quanta Analysis (Igor macro) by Mosharov et al. Full article
(This article belongs to the Special Issue Novel Biosensors for Cell Analysis)
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14 pages, 3376 KiB  
Article
Cell Patterning Technology on Polymethyl Methacrylate through Controlled Physicochemical and Biochemical Functionalization
by Enrique Azuaje-Hualde, Job Komen, Juncal A. Alonso-Cabrera, Albert van den Berg, Marian M. de Pancorbo, Andries D. van der Meer, Fernando Benito-Lopez and Lourdes Basabe-Desmonts
Biosensors 2023, 13(10), 904; https://doi.org/10.3390/bios13100904 - 23 Sep 2023
Viewed by 1271
Abstract
In recent years, innovative cell-based biosensing systems have been developed, showing impact in healthcare and life science research. Now, there is a need to design mass-production processes to enable their commercialization and reach society. However, current protocols for their fabrication employ materials that [...] Read more.
In recent years, innovative cell-based biosensing systems have been developed, showing impact in healthcare and life science research. Now, there is a need to design mass-production processes to enable their commercialization and reach society. However, current protocols for their fabrication employ materials that are not optimal for industrial production, and their preparation requires several chemical coating steps, resulting in cumbersome protocols. We have developed a simplified two-step method for generating controlled cell patterns on PMMA, a durable and transparent material frequently employed in the mass manufacturing of microfluidic devices. It involves air plasma and microcontact printing. This approach allows the formation of well-defined cell arrays on PMMA without the need for blocking agents to define the patterns. Patterns of various adherent cell types in dozens of individual cell cultures, allowing the regulation of cell–material and cell–cell interactions, were developed. These cell patterns were integrated into a microfluidic device, and their viability for more than 20 h under controlled flow conditions was demonstrated. This work demonstrated the potential to adapt polymeric cytophobic materials to simple fabrication protocols of cell-based microsystems, leveraging the possibilities for commercialization. Full article
(This article belongs to the Special Issue Novel Biosensors for Cell Analysis)
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9 pages, 2019 KiB  
Communication
Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells
by Valber A. Pedrosa, Kangfu Chen, Thomas J. George and Z. Hugh Fan
Biosensors 2023, 13(7), 706; https://doi.org/10.3390/bios13070706 - 04 Jul 2023
Cited by 1 | Viewed by 1397
Abstract
Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating [...] Read more.
Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% ± 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our method and platform enables the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs for biological assays. Full article
(This article belongs to the Special Issue Novel Biosensors for Cell Analysis)
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12 pages, 2550 KiB  
Article
Real-Time Monitoring the Cytotoxic Effect of Andrographolide on Human Oral Epidermoid Carcinoma Cells
by Heng-Yi Liao, Chun-Chung Huang, Shih-Chi Chao, Chien-Ping Chiang, Bo-Hsuan Tang, Shiao-Pieng Lee and Jehng-Kang Wang
Biosensors 2022, 12(5), 304; https://doi.org/10.3390/bios12050304 - 06 May 2022
Cited by 5 | Viewed by 1927
Abstract
Andrographolide is an active diterpenoid compound extracted from Andrographis paniculata. It exhibits antiinflammatory and anticancer effects. Previous studies show that it is non-toxic to experimental animals. The leading causes of cancer are chronic inflammation and high blood glucose. This study determines the [...] Read more.
Andrographolide is an active diterpenoid compound extracted from Andrographis paniculata. It exhibits antiinflammatory and anticancer effects. Previous studies show that it is non-toxic to experimental animals. The leading causes of cancer are chronic inflammation and high blood glucose. This study determines the cytotoxic effect of andrographolide on cellular morphology, viability, and migration for human oral epidermoid carcinoma cell Meng-1 (OEC-M1). We use electric cell-substrate impedance sensing (ECIS) to measure the subsequent overall impedance changes of the cell monolayer in response to different concentrations of andrographolide for 24 h (10–100 µM). The results for exposure of OEC-M1 cells to andrographolide (10–100 µM) for 24 h show a concentration-dependent decrease in the overall measured resistance at 4 kHz. AlamarBlue cell viability assay and annexin V also show the apoptotic effect of andrographolide on OEC-M1 cells. A reduction in wound-healing recovery rate is observed for cells treated with 30 μM andrographolide. This study demonstrates that ECIS can be used for the in vitro screening of anticancer drugs. ECIS detects the cytotoxic effect of drugs earlier than traditional biochemical assays, and it is more sensitive and shows more detail. Full article
(This article belongs to the Special Issue Novel Biosensors for Cell Analysis)
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Review

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19 pages, 3012 KiB  
Review
Introduction of Nanomaterials to Biosensors for Exosome Detection: Case Study for Cancer Analysis
by Myoungro Lee, Jinmyeong Kim, Moonbong Jang, Chulhwan Park, Jin-Ho Lee and Taek Lee
Biosensors 2022, 12(8), 648; https://doi.org/10.3390/bios12080648 - 17 Aug 2022
Cited by 6 | Viewed by 3713
Abstract
Exosomes have been gaining attention for early cancer diagnosis owing to their biological functions in cells. Several studies have reported the relevance of exosomes in various diseases, including pancreatic cancer, retroperitoneal fibrosis, obesity, neurodegenerative diseases, and atherosclerosis. Particularly, exosomes are regarded as biomarkers [...] Read more.
Exosomes have been gaining attention for early cancer diagnosis owing to their biological functions in cells. Several studies have reported the relevance of exosomes in various diseases, including pancreatic cancer, retroperitoneal fibrosis, obesity, neurodegenerative diseases, and atherosclerosis. Particularly, exosomes are regarded as biomarkers for cancer diagnosis and can be detected in biofluids, such as saliva, urine, peritoneal fluid, and blood. Thus, exosomes are advantageous for cancer liquid biopsies as they overcome the current limitations of cancer tissue biopsies. Several studies have reported methods for exosome isolation, and analysis for cancer diagnosis. However, further clinical trials are still required to determine accurate exosome concentration quantification methods. Recently, various biosensors have been developed to detect exosomal biomarkers, including tumor-derived exosomes, nucleic acids, and proteins. Among these, the exact quantification of tumor-derived exosomes is a serious obstacle to the clinical use of liquid biopsies. Precise detection of exosome concentration is difficult because it requires clinical sample pretreatment. To solve this problem, the use of the nanobiohybrid material-based biosensor provides improved sensitivity and selectivity. The present review will discuss recent progress in exosome biosensors consisting of nanomaterials and biomaterial hybrids for electrochemical, electrical, and optical-based biosensors. Full article
(This article belongs to the Special Issue Novel Biosensors for Cell Analysis)
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