Lab on a Chip Technology for Pathogen Detection and Disease Diagnosis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 March 2023) | Viewed by 9834

Special Issue Editors

Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA
Interests: micro/nanofluidics; micro–nanofabrication; lab on a chip; cell separation; microfluidic technology; acousto/magneto/electro-microfluidics
Special Issues, Collections and Topics in MDPI journals
ZJU-UIUC Institute, International Campus, Zhejiang University, Haining 314400, China
Interests: advanced nanomanufacturing; bio-inspired sensing; micro/nano-sensors; lab on chip
Special Issues, Collections and Topics in MDPI journals
Beijing Advanced Innovation Center for Biomedical Engineering, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, China
Interests: single cell nanoengineering; biosensor and POCT devices; wearable flexible biosensors

Special Issue Information

Dear Colleagues,

The past two years have witnessed the battle between humans and pandemics and the urgency and importance of detecting pathogens rapidly has become clear. The ability to detect disease in the early stages can also result in higher chances of survival. Benefitting from miniaturization and automation, lab-on-a-chip technology is playing an increasingly fundamental role in pathogen detection and disease diagnosis by offering biosensors with higher sensitivity, lower costs, and the potential to be mass-produced.

This Special Issue calls for papers about new sensing mechanisms, new materials, new micro/nanofabrication processes, new devices, and new applications in lab-on-a-chip technology that are helpful for detecting pathogens such as viruses and bacteria, as well as for enabling disease diagnosis via detecting antibodies, DNA, RNA, exosomes, and cells. All original research or review articles are welcome on both the fundamentals, fabrication, and applications of micro/nanoscale biosensors. Potential topics include (but are not limited to):

  • Micro/nano biosensing;
  • Microfluidics and nanofluidic biosensors;
  • Micro and nanofabrication;
  • Lab-on-a-chip sensors;
  • Electrochemical sensing;
  • Plasmonic biosensors;
  • Physiological signal detection;
  • Biosensors for cells, viruses, and bacteria;
  • Exosome isolation and detection;
  • Flexible and wearable electronic biosensors;
  • Novel materials, e.g., 2D metamaterials and liquid metals, for biosensing;
  • Field effect transistors (FET) for biosensing.

Dr. Cheng Wang
Dr. Huan Hu
Prof. Dr. Lingqian Chang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lab on a chip sensors
  • micro/nano-fluidic biosensors
  • optofluidic sensing
  • micro/nano biosensing
  • flexible wearable sensors
  • new materials
  • point of care sensors
  • rapid disease detection
  • pathogen sensors

Published Papers (3 papers)

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Research

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22 pages, 8641 KiB  
Article
Interrogation on the Cellular Nano-Interface and Biosafety of Repeated Nano-Electroporation by Nanostraw System
by Aihua Zhang, Jiaru Fang, Ji Wang, Xi Xie, Hui-Jiuan Chen and Gen He
Biosensors 2022, 12(7), 522; https://doi.org/10.3390/bios12070522 - 13 Jul 2022
Cited by 2 | Viewed by 1722
Abstract
Cell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system could allow [...] Read more.
Cell perforation is a critical step for intracellular drug delivery and real-time biosensing of intracellular signals. In recent years, the nanostraws system has been developed to achieve intracellular drug delivery with minimal invasiveness to the cells. Repeated cell perforation via nano-system could allow delivery of multiple drugs into cells for cell editing, but the biosafety is rarely explored. In this work, a nanostraw-mediated nano-electroporation system was developed, which allowed repeated perforation of the same set of cells in a minimally invasive manner, while the biosafety aspect of this system was investigated. Highly controllable fabrication of Al2O3 nanostraw arrays based on a porous polyethylene terephthalate (PET) membrane was integrated with a microfluidic device to construct the nanostraw-electroporation system. The pulse conditions and intervals of nano-electroporation were systematically optimized to achieve efficient cells perforation and maintain the viability of the cells. The cells proliferation, the early apoptosis activities after nanostraw-electroporation and the changes of gene functions and gene pathways of cells after repeated nano-electroporation were comprehensively analyzed. These results revealed that the repeated nanostraw-electroporation did not induce obvious negative effects on the cells. This work demonstrates the feasibility of repeated nano-electroporation on cells and provides a promising strategy for future biomedical applications. Full article
(This article belongs to the Special Issue Lab on a Chip Technology for Pathogen Detection and Disease Diagnosis)
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13 pages, 1963 KiB  
Article
Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification
by Yu-Ting Kao, Silvia Calabrese, Nadine Borst, Michael Lehnert, Yu-Kai Lai, Franziska Schlenker, Peter Juelg, Roland Zengerle, Piotr Garstecki and Felix von Stetten
Biosensors 2022, 12(4), 237; https://doi.org/10.3390/bios12040237 - 12 Apr 2022
Cited by 3 | Viewed by 3570
Abstract
We demonstrate detection and quantification of bacterial load with a novel microfluidic one-pot wash-free fluorescence in situ hybridization (FISH) assay in droplets. The method offers minimal manual workload by only requiring mixing of the sample with reagents and loading it into a microfluidic [...] Read more.
We demonstrate detection and quantification of bacterial load with a novel microfluidic one-pot wash-free fluorescence in situ hybridization (FISH) assay in droplets. The method offers minimal manual workload by only requiring mixing of the sample with reagents and loading it into a microfluidic cartridge. By centrifugal microfluidic step emulsification, our method partitioned the sample into 210 pL (73 µm in diameter) droplets for bacterial encapsulation followed by in situ permeabilization, hybridization, and signal detection. Employing locked nucleic acid (LNA)/DNA molecular beacons (LNA/DNA MBs) and NaCl-urea based hybridization buffer, the assay was characterized with Escherichia coli, Klebsiella pneumonia, and Proteus mirabilis. The assay performed with single-cell sensitivity, a 4-log dynamic range from a lower limit of quantification (LLOQ) at ~3 × 103 bacteria/mL to an upper limit of quantification (ULOQ) at ~3 × 107 bacteria/mL, anda linearity R2 = 0.976. The total time-to-results for detection and quantification was around 1.5 hours. Full article
(This article belongs to the Special Issue Lab on a Chip Technology for Pathogen Detection and Disease Diagnosis)
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15 pages, 31741 KiB  
Perspective
Preparation and Applications of Electrospun Nanofibers for Wearable Biosensors
by Tengzhou Xu, Guojing Ji, Hui Li, Jiaduo Li, Zhou Chen, Desire Emefa Awuye and Jie Huang
Biosensors 2022, 12(3), 177; https://doi.org/10.3390/bios12030177 - 17 Mar 2022
Cited by 9 | Viewed by 3539
Abstract
The emergence of nanotechnology has provided many new ideas and innovations in the field of biosensors. Electrospun nanofibers have many excellent properties such as high specific surface area, high porosity, low cost, high efficiency, and they can be combined with a variety of [...] Read more.
The emergence of nanotechnology has provided many new ideas and innovations in the field of biosensors. Electrospun nanofibers have many excellent properties such as high specific surface area, high porosity, low cost, high efficiency, and they can be combined with a variety of sensors. These remarkable features have a wide range of applications in the field of sensors such as monitoring air pollutants, highly sensitive pressure sensors, and biosensors for monitoring the pulse of the body. This paper summarizes the working principle and influencing factors of electrospinning nanofibers, and illustrates their applications in wearable biosensors. Full article
(This article belongs to the Special Issue Lab on a Chip Technology for Pathogen Detection and Disease Diagnosis)
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