Advances of Genetically Encoded Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: closed (31 October 2021) | Viewed by 4650

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Guest Editor
Department of Anatomy and Structural Biology and Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Interests: protein engineering; near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs); genetically encoded biosensors and optogenetic tools
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Special Issue Information

Dear Colleagues,

Genetically Encoded Biosensors (GEBs) have revolutionized modern science. It was always a challenge for scientists to sense and measure biochemical changes within a cellular compartment or within a specific cell type in a transgenic organism without perturbing the metabolism of the experimental subject. After the discovery of fluorescent and bioluminescent proteins, as well as bringing microscopy and directed molecular evolution techniques to a new level, the development of advanced genetically encoded biosensors became possible. Scientists from around the world have put tremendous effort into the creation of the GEBs that measure concentrations and activity levels of molecules or ions. Steadily, the research arrives to a point when genetically encoded biosensors will be routinely used to monitor long-term signaling processes during the development, disease progression, and aging of a transgenic organism.

We encourage you to share your findings and ideas in the field of genetically encoded biosensors. This Special Issue will cover all possible types of current GEBs, challenges in their design, a plethora of their applications across scales, their multiplexing, tools required to work with GEBs, and future outlooks. In light of the COVID-19 pandemic, researchers are welcome to share their ideas and discoveries on how genetically encoded biosensors could be used in drug discovery.

Dr. Mikhail Baloban
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • genetically encoded biosensors (GEBs)
  • fluorescent proteins (FPs)
  • bioluminescent proteins (BPs)
  • sensing unit (SU)

Published Papers (1 paper)

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Research

11 pages, 1687 KiB  
Article
FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor
by Alexander S. Goryashchenko, Alexey A. Pakhomov, Anastasia V. Ryabova, Igor D. Romanishkin, Eugene G. Maksimov, Alexander N. Orsa, Oxana V. Serova, Andrey A. Mozhaev, Margarita A. Maksimova, Vladimir I. Martynov, Alexander G. Petrenko and Igor E. Deyev
Biosensors 2021, 11(9), 340; https://doi.org/10.3390/bios11090340 - 15 Sep 2021
Cited by 11 | Viewed by 3731
Abstract
The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH [...] Read more.
The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extracellular media pH in a mildly alkaline range. SypHerExtra is a protein created by fusing the previously described pH sensor SypHer3s with the neurexin transmembrane domain that targets its expression to the cytoplasmic membrane. We showed that with excitation at 445 nm, the fluorescence lifetime of both SypHer3s and SypHerExtra strongly depend on pH. Using FLIM microscopy in live eukaryotic cells, we demonstrated that SypHerExtra can be successfully used to determine extracellular pH, while SypHer3s can be applied to measure intracellular pH. Thus, these two sensors are suitable for quantitative measurements using the FLIM method, to determine intracellular and extracellular pH in a range from pH 7.5 to 9.5 in different biological systems. Full article
(This article belongs to the Special Issue Advances of Genetically Encoded Biosensors)
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