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Biosensors
Special Issues
Electrochemical Biosensors for Biomedical Applications
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Electrochemical Biosensors for Biomedical Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (20 June 2023) | Viewed by 15179

Special Issue Editors

Prof. Dr. Yun Seok Heo

E-Mail Website
Guest Editor
Department of Biomedical Enginering, School of Medicine, Keimyung University, Daegu 42601, Republic of Korea
Interests: electrochemical biosensor; microfluidics; cell culture and assay, in vitro fertilization (IVF); point of care testing (POCT)
Dr. Yoo Min Park

E-Mail Website
Guest Editor
Center for NanoBio Development, National NanoFab Center, Daejeon 34141, Korea
Interests: electrochemical biosensor; immunoassay; PCR; microfluidics; nano-based electrode; photolithography; point of care testing (POCT)

Special Issue Information

Dear Colleagues,

The electrochemical biosensor is a typical sensing device that transduces biochemical events such as enzyme substrate reaction, DNA/RNA or aptamer conjugation, and antigen–antibody interaction in electrical signals (e.g., current, voltage, impedance). For the last few decades, electrochemical biosensors have played an important role in the improvement of public health by monitoring various biochemical compounds or biomarkers in body. For example, biomarkers can be any metabolites such as protein, lipid, nucleic acid, and carbohydrate present in easily collectable clinical samples. If any biometabolites change their status from the normal range in the presence of any disease conditions, they can be used as a biomarker for a given disease condition. Thus, we believe that electrochemical biosensors will make health tests more affordable and easily accessible and offer promising technological options to biomedical diagnostics. This Special Issue seeks to address the latest and most important advances in electrochemical biosensors for biomedical applications. Manuscripts including original research articles and reviews are welcomed.

This Special Issue of Biosensors will focus on the following topics related to electrochemical biosensors but not limited to:

  • Detection of cancer, metabolic or any disease biomarkers;
  • Diagnosis for clinical applications;
  • Immunosensors;
  • Label-free biosensors;
  • Enzyme-based biosensors;
  • DNA-based biosensors;
  • Employing functional nanomaterials;
  • Miniaturized systems;
  • Biosensors in lab on a chip (LOC);
  • Biosensors in organ on a chip (OOC);
  • Point-of-care testing (POCT);
  • Implantable biomedical devices;
  • Immobilization of biomolecules (enzyme, antibody and nucleic acid, etc.);
  • Chemical modification methods on electrodes;
  • Signal-enhancing technologies.

Prof. Dr. Yun Seok Heo
Dr. Yoo Min Park
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Electrochemcial biosensors
  • Biomarkers
  • Diease diagnosis
  • Clinical applications
  • Immunosensors
  • Label free
  • Lab on a Chip (LOC)
  • Point-Of-Care-Testing (POCT)
  • Functional nanomaterials
  • Immobilization of biomolecules
  • Signal Enhancements

Published Papers (5 papers)

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Research

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15 pages, 3057 KiB  
Article
A Flexible and Transparent PtNP/SWCNT/PET Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released from Living Cells with Real-Time Monitoring Capability
by Da Eun Oh, Chang-Seuk Lee, Tae Wan Kim, Seob Jeon and Tae Hyun Kim
Biosensors 2023, 13(7), 704; https://doi.org/10.3390/bios13070704 - 03 Jul 2023
Cited by 3 | Viewed by 1327
Abstract
We developed a transparent and flexible electrochemical sensor using a platform based on a network of single-walled carbon nanotubes (SWCNTs) for the non-enzymatic detection of hydrogen peroxide (H2O2) released from living cells. We decorated the SWCNT network on a [...] Read more.
We developed a transparent and flexible electrochemical sensor using a platform based on a network of single-walled carbon nanotubes (SWCNTs) for the non-enzymatic detection of hydrogen peroxide (H2O2) released from living cells. We decorated the SWCNT network on a poly(ethylene terephthalate) (PET) substrate with platinum nanoparticles (PtNPs) using a potentiodynamic method. The PtNP/SWCNT/PET sensor synergized the advantages of a flexible PET substrate, a conducting SWCNT network, and a catalytic PtNP and demonstrated good biocompatibility and flexibility, enabling cell adhesion. The PtNP/SWCNT/PET-based sensor demonstrated enhanced electrocatalytic activity towards H2O2, as well as excellent selectivity, stability, and reproducibility. The sensor exhibited a wide dynamic range of 500 nM to 1 M, with a low detection limit of 228 nM. Furthermore, the PtNP/SWCNT/PET sensor remained operationally stable, even after bending at various angles (15°, 30°, 60°, and 90°), with no noticeable loss of current signal. These outstanding characteristics enabled the PtNP/SWCNT/PET sensor to be practically applied for the direct culture of HeLa cells and the real-time monitoring of H2O2 release by the HeLa cells under drug stimulation. Full article
(This article belongs to the Special Issue Electrochemical Biosensors for Biomedical Applications)
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14 pages, 8653 KiB  
Article
Diboronic-Acid-Based Electrochemical Sensor for Enzyme-Free Selective and Sensitive Glucose Detection
by Joong-Hyun Kim, Hongsik Choi, Chul-Soon Park, Heung-Seop Yim, Dongguk Kim, Sungmin Lee and Yeonkeong Lee
Biosensors 2023, 13(2), 248; https://doi.org/10.3390/bios13020248 - 09 Feb 2023
Cited by 5 | Viewed by 3800
Abstract
A diboronic acid anthracene-based fluorescent system for detecting blood glucose could be used for 180 days. However, there has not yet been a boronic acid immobilized electrode to selectively detect glucose in a signal-increased way. Considering malfunctions of sensors at high sugar levels, [...] Read more.
A diboronic acid anthracene-based fluorescent system for detecting blood glucose could be used for 180 days. However, there has not yet been a boronic acid immobilized electrode to selectively detect glucose in a signal-increased way. Considering malfunctions of sensors at high sugar levels, the electrochemical signal should be increased proportionally to the glucose concentration. Therefore, we synthesized a new diboronic acid derivative and fabricated the derivative-immobilized electrodes for the selective detection of glucose. We performed cyclic voltammetry and electrochemical impedance spectroscopy with an Fe(CN)63−/4− redox pair for detecting glucose in the range of 0–500 mg/dL. The analysis revealed increased electron-transfer kinetics such as increased peak current and decreased semicircle radius of Nyquist plots as the glucose concentration increased. The cyclic voltammetry and impedance spectroscopy showed that the linear detection range of glucose was 40 to 500 mg/dL with limits of detection of 31.2 mg/dL and 21.5 mg/dL, respectively. We applied the fabricated electrode to detect glucose in artificial sweat and obtained 90% of the performance of the electrodes in PBS. Cyclic voltammetry measurements of other sugars such as galactose, fructose, and mannitol also showed linear increased peak currents proportional to the concentrations of the tested sugars. However, the slopes of the sugars were lower than that of glucose, indicating selectivity for glucose. These results proved the newly synthesized diboronic acid is a promising synthetic receptor for developing a long-term usable electrochemical sensor system. Full article
(This article belongs to the Special Issue Electrochemical Biosensors for Biomedical Applications)
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15 pages, 5092 KiB  
Article
Tri-Channel Electrochemical Immunobiosensor for Combined Detections of Multiple Exosome Biomarkers of Lung Cancer
by Cui Fan, Bingyan Jiang, Wenjia Shi, Dan Chen and Mingyong Zhou
Biosensors 2022, 12(7), 435; https://doi.org/10.3390/bios12070435 - 21 Jun 2022
Cited by 4 | Viewed by 2170
Abstract
Current methods for the early diagnosis of cancer can be invasive and costly. In recent years, exosomes have been recognized as potential biomarkers for cancer diagnostics. The common methods for quantitative detection of exosomes, such as nanoparticle tracking analysis (NTA) and flow cytometry, [...] Read more.
Current methods for the early diagnosis of cancer can be invasive and costly. In recent years, exosomes have been recognized as potential biomarkers for cancer diagnostics. The common methods for quantitative detection of exosomes, such as nanoparticle tracking analysis (NTA) and flow cytometry, rely on large-scale instruments and complex operation, with results not specific for cancer. Herein, we present a tri-channel electrochemical immunobiosensor for enzyme-free and label-free detecting carcino-embryonic antigen (CEA), neuron-specific enolase (NSE), and cytokeratin 19 fragments (Cyfra21-1) from exosomes for specific early diagnosis of lung cancer. The electrochemical immunobiosensor showed good selectivity and stability. Under optimum experimental conditions, the linear ranges were from 10−3 to 10 ng/mL for CEA, 10−4 to 102 ng/mL for NSE, and 10−3 to 102 ng/mL for Cyfra21-1, and a detection limit down to 10−4 ng/mL was achieved. Furthermore, we performed exosome analysis in three kinds of lung cancer. The results showed a distinct expression level of exosomal markers in different types. These works provide insight into a promising alternative for the quantification of exosomal markers in specific diseases in the following clinical bioassays. Full article
(This article belongs to the Special Issue Electrochemical Biosensors for Biomedical Applications)
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14 pages, 2827 KiB  
Article
A Dual Electrode Biosensor for Glucose and Lactate Measurement in Normal and Prolonged Obese Mice Using Single Drop of Whole Blood
by Mukesh Thapa, Ryong Sung and Yun Seok Heo
Biosensors 2021, 11(12), 507; https://doi.org/10.3390/bios11120507 - 09 Dec 2021
Cited by 11 | Viewed by 3152
Abstract
Understanding the levels of glucose (G) and lactate (L) in blood can help us regulate various chronic health conditions such as obesity. In this paper, we introduced an enzyme-based electrochemical biosensor adopting glucose oxidase and lactate oxidase on two working screen-printed carbon electrodes [...] Read more.
Understanding the levels of glucose (G) and lactate (L) in blood can help us regulate various chronic health conditions such as obesity. In this paper, we introduced an enzyme-based electrochemical biosensor adopting glucose oxidase and lactate oxidase on two working screen-printed carbon electrodes (SPCEs) to sequentially determine glucose and lactate concentrations in a single drop (~30 µL) of whole blood. We developed a diet-induced obesity (DIO) mouse model for 28 weeks and monitored the changes in blood glucose and lactate levels. A linear calibration curve for glucose and lactate concentrations in ranges from 0.5 to 35 mM and 0.5 to 25 mM was obtained with R-values of 0.99 and 0.97, respectively. A drastic increase in blood glucose and a small but significant increase in blood lactate were seen only in prolonged obese cases. The ratio of lactate concentration to glucose concentration (L/G) was calculated as the mouse’s gained weight. The results demonstrated that an L/G value of 0.59 could be used as a criterion to differentiate between normal and obesity conditions. With L/G and weight gain, we constructed a diagnostic plot that could categorize normal and obese health conditions into four different zones. The proposed dual electrode biosensor for glucose and lactate in mouse whole blood showed good stability, selectivity, sensitivity, and efficiency. Thus, we believe that this dual electrode biosensor and the diagnostic plot could be used as a sensitive analytical tool for diagnosing glucose and lactate biomarkers in clinics and for monitoring obesity. Full article
(This article belongs to the Special Issue Electrochemical Biosensors for Biomedical Applications)
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Review

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22 pages, 19132 KiB  
Review
Design and Preparation of Sensing Surfaces for Capacitive Biodetection
by Perrine Robin and Sandrine Gerber-Lemaire
Biosensors 2023, 13(1), 17; https://doi.org/10.3390/bios13010017 - 23 Dec 2022
Cited by 3 | Viewed by 2393
Abstract
Despite their high sensitivity and their suitability for miniaturization, biosensors are still limited for clinical applications due to the lack of reproducibility and specificity of their detection performance. The design and preparation of sensing surfaces are suspected to be a cause of these [...] Read more.
Despite their high sensitivity and their suitability for miniaturization, biosensors are still limited for clinical applications due to the lack of reproducibility and specificity of their detection performance. The design and preparation of sensing surfaces are suspected to be a cause of these limitations. Here, we first present an updated overview of the current state of use of capacitive biosensors in a medical context. Then, we summarize the encountered strategies for the fabrication of capacitive biosensing surfaces. Finally, we describe the characteristics which govern the performance of the sensing surfaces, along with recent developments that were suggested to overcome their main current limitations. Full article
(This article belongs to the Special Issue Electrochemical Biosensors for Biomedical Applications)
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