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Biosensors
Special Issues
State-of-the-Art Biosensors in Australia
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State-of-the-Art Biosensors in Australia

A special issue of Biosensors (ISSN 2079-6374).

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 14506

Special Issue Editor

Prof. Dr. Samuel Adeloju

E-Mail Website
Guest Editor
School of Chemistry, Monash University, Clayton, VIC 3800, Australia
Interests: nanobiosensors; nanomaterials; conducting polymers; electroanalytical chemistry; environmental chemistry; microbial fuel cells
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Australia is recognized globally as one of the leading countries in biosensor research. To showcase the breadth, depth, and quality of this research, we are planning to publish a Special Issue, entitled “State-of-the-Art Biosensors in Australia”. This regional project aims to collect high-quality research articles, review articles, and communications on all aspects of biosensors and biosensing from Australia. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. The topic would include but not be limited to the following themes:

  • DNA chips;
  • Lab-on-a-chip technology;
  • Microfluidic devices;
  • Nanobiosensors and nanotechnology used in biosensors;
  • Biosensor fabrication;
  • Biomaterials;
  • Biosensor interfaces and membrane technology;
  • In vitro and in vivo applications;
  • Instrumentation, signal treatment, and uncertainty estimation in biosensors.

I encourage you to join me in this Special Issue to showcase the best biosensor research in Australia to the rest of the world through this high-impact journal.

Prof. Dr. Samuel B. Adeloju
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (5 papers)

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Research

Jump to: Review

17 pages, 4393 KiB  
Article
Biofunctionalisation of Polypyrrole Nanowires Array with Sulfite Oxidase Coupled with the Integration of Platinum Nanoparticles for Ultrasensitive Amperometric Detection of Sulfite
by Shahid Hussain and Samuel B. Adeloju
Biosensors 2023, 13(6), 621; https://doi.org/10.3390/bios13060621 - 05 Jun 2023
Cited by 1 | Viewed by 1043
Abstract
Sulfite determination in foods and alcoholic beverages is a common requirement by food and drug administration organisations in most countries. In this study, the enzyme, sulfite oxidase (SOx), is used to biofunctionalise a platinum-nanoparticle-modified polypyrrole nanowire array (PPyNWA) for the ultrasensitive amperometric detection [...] Read more.
Sulfite determination in foods and alcoholic beverages is a common requirement by food and drug administration organisations in most countries. In this study, the enzyme, sulfite oxidase (SOx), is used to biofunctionalise a platinum-nanoparticle-modified polypyrrole nanowire array (PPyNWA) for the ultrasensitive amperometric detection of sulfite. A dual-step anodisation method was used to prepare the anodic aluminum oxide membrane used as a template for the initial fabrication of the PPyNWA. PtNPs were subsequently deposited on the PPyNWA by potential cycling in a platinum solution. The resulting PPyNWA-PtNP electrode was then biofuntionalised by adsorption of SOx onto the surface. The confirmation of the adsorption of SOx and the presence of PtNPs in the PPyNWA-PtNPs-SOx biosensor was verified by scanning electron microscopy and electron dispersive X-ray spectroscopy. Cyclic voltammetry and amperometric measurements were used to investigate the properties of the nanobiosensor and to optimise its use for sulfite detection. Ultrasensitive detection of sulfite with the PPyNWA-PtNPs-SOx nanobiosensor was accomplished by use of 0.3 M pyrrole, 10 U mL−1 of SOx, adsorption time of 8 h, a polymerisation period of 900 s, and an applied current density of 0.7 mA cm−2. The response time of the nanobiosensor was 2 s, and its excellent analytical performance was substantiated with a sensitivity of 57.33 μA cm−2 mM−1, a limit of detection of 12.35 nM, and a linear response range from 0.12 to 1200 μM. Application of the nanobiosensor to sulfite determination in beer and wine samples was achieved with a recovery efficiency of 97–103%. Full article
(This article belongs to the Special Issue State-of-the-Art Biosensors in Australia)
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12 pages, 2111 KiB  
Article
Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals
by Amane Shiohara, Christopher D. Easton, Beatriz Prieto-Simon and Nicolas H. Voelcker
Biosensors 2022, 12(7), 480; https://doi.org/10.3390/bios12070480 - 30 Jun 2022
Cited by 1 | Viewed by 1781
Abstract
Rapid, sensitive, selective and portable virus detection is in high demand globally. However, differentiating non-infectious viral particles from intact/infectious viruses is still a rarely satisfied sensing requirement. Using the negative space within monolayers of polystyrene (PS) spheres deposited directly on gold electrodes, we [...] Read more.
Rapid, sensitive, selective and portable virus detection is in high demand globally. However, differentiating non-infectious viral particles from intact/infectious viruses is still a rarely satisfied sensing requirement. Using the negative space within monolayers of polystyrene (PS) spheres deposited directly on gold electrodes, we fabricated tuneable nanochannels decorated with target-selective bioreceptors that facilitate the size-selective detection of intact viruses. Detection occurred through selective nanochannel blockage of diffusion of a redox probe, [Fe(CN)6]3/4−, allowing a quantifiable change in the oxidation current before and after analyte binding to the bioreceptor immobilised on the spheres. Our model system involved partial surface passivation of the mono-assembled PS spheres, by silica glancing angle deposition, to confine bioreceptor immobilisation specifically to the channels and improve particle detection sensitivity. Virus detection was first optimised and modelled with biotinylated gold nanoparticles, recognised by streptavidin immobilised on the PS layer, reaching a low limit of detection of 37 particles/mL. Intact, label-free virus detection was demonstrated using MS2 bacteriophage (~23–28 nm), a marker of microbiological contamination, showing an excellent limit of detection of ~1.0 pfu/mL. Tuneable nanochannel geometries constructed directly on sensing electrodes offer label-free, sensitive, and cost-efficient point-of-care biosensing platforms that could be applied for a wide range of viruses. Full article
(This article belongs to the Special Issue State-of-the-Art Biosensors in Australia)
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13 pages, 1646 KiB  
Article
An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples
by Kimberley Clack, Narshone Soda, Surasak Kasetsirikul, Richard Kline, Carlos Salomon and Muhammad J. A. Shiddiky
Biosensors 2022, 12(5), 287; https://doi.org/10.3390/bios12050287 - 28 Apr 2022
Cited by 2 | Viewed by 2732
Abstract
Long non-coding RNA Homeobox transcript antisense intergenic RNA (HOTAIR) is recognized as a participant in different processes of normal cell development. Aberrant overexpression of HOTAIR contributes to the initiation, growth, and invasiveness of ovarian cancer. Using the affinity interaction of target HOTAIR lncRNA [...] Read more.
Long non-coding RNA Homeobox transcript antisense intergenic RNA (HOTAIR) is recognized as a participant in different processes of normal cell development. Aberrant overexpression of HOTAIR contributes to the initiation, growth, and invasiveness of ovarian cancer. Using the affinity interaction of target HOTAIR lncRNA sequences towards a screen-printed gold electrode (SPE-Au), herein we report on a novel, rapid and simple method to detect HOTAIR sequences. HOTAIR lncRNA sequences were first extracted from ovarian cancer cell lines and patient plasma samples and were magnetically captured and purified by complimentary capture probe-functionalized magnetic beads. Isolated target HOTAIR lncRNAs were directly adsorbed onto unmodified screen-printed gold electrodes (SPE-Au) for direct quantification with [Fe(CN)6]3−/4− redox couple. Our assay achieved a linear dynamic range of 100 nM and 1 pM for detecting pre-clinical model HOTAIR lncRNA samples (%RSD ≤ 5%, for n = 3) and was highly specific, showing clear distinction between HOTAIR lncRNA targets and non-specific miR-891 and miR-486 (100 nM) (%RSD ≤ 5%, for n = 3). The method was tested using ovarian cancer-specific cell lines (SKOV3 and OVCAR3) and mesothelial cell line (MeT-5A)-derived lncRNAs. The analytical performance of our method was validated using RT-qPCR. Finally, the method was tested using clinical samples from ovarian cancer patients and the resulting electrochemical responses show a clear distinction between the ovarian carcinoma and benign samples. Full article
(This article belongs to the Special Issue State-of-the-Art Biosensors in Australia)
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Review

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48 pages, 6594 KiB  
Review
Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review
by Soo Min Lee, Hari Kalathil Balakrishnan, Egan H. Doeven, Dan Yuan and Rosanne M. Guijt
Biosensors 2023, 13(11), 980; https://doi.org/10.3390/bios13110980 - 10 Nov 2023
Cited by 1 | Viewed by 2318
Abstract
Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to protect the public and ecosystems from widespread outbreaks of biological [...] Read more.
Nucleic acid amplification testing facilitates the detection of disease through specific genomic sequences and is attractive for point-of-need testing (PONT); in particular, the early detection of microorganisms can alert early response systems to protect the public and ecosystems from widespread outbreaks of biological threats, including infectious diseases. Prior to nucleic acid amplification and detection, extensive sample preparation techniques are required to free nucleic acids and extract them from the sample matrix. Sample preparation is critical to maximize the sensitivity and reliability of testing. As the enzymatic amplification reactions can be sensitive to inhibitors from the sample, as well as from chemicals used for lysis and extraction, avoiding inhibition is a significant challenge, particularly when minimising liquid handling steps is also desirable for the translation of the assay to a portable format for PONT. The reagents used in sample preparation for nucleic acid testing, covering lysis and NA extraction (binding, washing, and elution), are reviewed with a focus on their suitability for use in PONT. Full article
(This article belongs to the Special Issue State-of-the-Art Biosensors in Australia)
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25 pages, 5090 KiB  
Review
Wearable Smart Bandage-Based Bio-Sensors
by Arie Levin, Shu Gong and Wenlong Cheng
Biosensors 2023, 13(4), 462; https://doi.org/10.3390/bios13040462 - 06 Apr 2023
Cited by 5 | Viewed by 5163
Abstract
Bandage is a well-established industry, whereas wearable electronics is an emerging industry. This review presents the bandage as the base of wearable bioelectronics. It begins with introducing a detailed background to bandages and the development of bandage-based smart sensors, which is followed by [...] Read more.
Bandage is a well-established industry, whereas wearable electronics is an emerging industry. This review presents the bandage as the base of wearable bioelectronics. It begins with introducing a detailed background to bandages and the development of bandage-based smart sensors, which is followed by a sequential discussion of the technical characteristics of the existing bandages, a more practical methodology for future applications, and manufacturing processes of bandage-based wearable biosensors. The review then elaborates on the advantages of basing the next generation of wearables, such as acceptance by the customers and system approvals, and disposal. Full article
(This article belongs to the Special Issue State-of-the-Art Biosensors in Australia)
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