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Biosensors
Special Issues
Bioluminescent Biosensors for Biomedical Applications
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Special Issue "Bioluminescent Biosensors for Biomedical Applications"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 7648

Special Issue Editor

Prof. Dr. Saman Hosseinkhani

E-Mail Website
Guest Editor
Department of Biochemistry, Tarbiat Modares University, Tehran 14115-111, Iran
Interests: bioluminescence; protein engineering; cell death protein complexes; luciferase

Special Issue Information

Dear Colleagues,

I am pleased to invite contributions to this Special Issue covering bioluminescence-based biosensors in all medical fields. Their high sensitivity, low background, and broad linear range of detection make bioluminescent biosensors valuable tools for different biomedical purposes, ranging from the detection of metabolites in biological fluids to evaluating the progression of diseases at the molecular level. This has been apparent in the volume of papers in the more recent success of many commercial devices.

Bioluminescence-based biosensors are exceptionally diverse. They attract interest and contributions from physicists, chemists and biologists, as well as electrical, mechanical, chemical, and biomedical engineers, with wide applications in biomedicine. Various community attitudes and numerous challenges emerge from different training, terminology, standards and opportunities for data.  Nonetheless, we may achieve great success if we can overcome these differences and communicate effectively.

I invite contributions to this Special Issue from as broad a community as possible. The list of potential topics is vast, but we are particularly interested in works that elucidate how some biological phenomena are leveraged by bioluminescent biosensors to improve diagnostic applications, drug screening and bioimaging approaches.

I invite the submission of scientifically sound work that is presented in clear, concise, and simple writing. The work should be thoughtfully analyzed and written for the biological and biomedical communities.

Prof. Dr. Saman Hosseinkhani
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioluminescence
  • biosensor
  • biomedicine
  • imaging
  • cancer
  • therapy
  • neurodegenerative diseases
  • protein–protein interaction
  • cell death

Published Papers (3 papers)

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Research

14 pages, 2840 KiB  
Article
Bioluminescent RIPoptosome Assay for FADD/RIPK1 Interaction Based on Split Luciferase Assay in a Human Neuroblastoma Cell Line SH-SY5Y
by
Parisa Ghanavatian
,
Hossein Salehi-Sedeh
,
Farangis Ataei
and
Saman Hosseinkhani
Biosensors 2023, 13(2), 297; https://doi.org/10.3390/bios13020297 - 20 Feb 2023
Cited by 1 | Viewed by 1992
Abstract
Different programed cell death (PCD) modalities involve protein–protein interactions in large complexes. Tumor necrosis factor α (TNFα) stimulated assembly of receptor-interacting protein kinase 1 (RIPK1)/Fas-associated death domain (FADD) interaction forms Ripoptosome complex that may cause either apoptosis or necroptosis. The present study addresses [...] Read more.
Different programed cell death (PCD) modalities involve protein–protein interactions in large complexes. Tumor necrosis factor α (TNFα) stimulated assembly of receptor-interacting protein kinase 1 (RIPK1)/Fas-associated death domain (FADD) interaction forms Ripoptosome complex that may cause either apoptosis or necroptosis. The present study addresses the interaction of RIPK1 and FADD in TNFα signaling by fusion of C-terminal (CLuc) and N-terminal (NLuc) luciferase fragments to RIPK1-CLuc (R1C) or FADD-NLuc (FN) in a caspase 8 negative neuroblastic SH-SY5Y cell line, respectively. In addition, based on our findings, an RIPK1 mutant (R1C K612R) had less interaction with FN, resulting in increasing cell viability. Moreover, presence of a caspase inhibitor (zVAD.fmk) increases luciferase activity compared to Smac mimetic BV6 (B), TNFα -induced (T) and non-induced cell. Furthermore, etoposide decreased luciferase activity, but dexamethasone was not effective in SH-SY5Y. This reporter assay might be used to evaluate basic aspects of this interaction as well as for screening of necroptosis and apoptosis targeting drugs with potential therapeutic application. Full article
(This article belongs to the Special Issue Bioluminescent Biosensors for Biomedical Applications)
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17 pages, 4205 KiB  
Article
Label-Free and Bioluminescence-Based Nano-Biosensor for ATP Detection
by
Elham Karimi
,
Maryam Nikkhah
and
Saman Hosseinkhani
Biosensors 2022, 12(11), 918; https://doi.org/10.3390/bios12110918 - 24 Oct 2022
Cited by 2 | Viewed by 2033
Abstract
A bioluminescence-based assay for ATP can measure cell viability. Higher ATP concentration indicates a higher number of living cells. Thus, it is necessary to design an ATP sensor that is low-cost and easy to use. Gold nanoparticles provide excellent biocompatibility for enzyme immobilization. [...] Read more.
A bioluminescence-based assay for ATP can measure cell viability. Higher ATP concentration indicates a higher number of living cells. Thus, it is necessary to design an ATP sensor that is low-cost and easy to use. Gold nanoparticles provide excellent biocompatibility for enzyme immobilization. We investigated the effect of luciferase proximity with citrate-coated gold, silver, and gold–silver core–shell nanoparticles, gold nanorods, and BSA–Au nanoclusters. The effect of metal nanoparticles on the activity of luciferases was recorded by the luminescence assay, which was 3–5 times higher than free enzyme. The results showed that the signal stability in presence of nanoparticles improved and was reliable up to 6 h for analytes measurements. It has been suggested that energy is mutually transferred from luciferase bioluminescence spectra to metal nanoparticle surface plasmons. In addition, we herein report the 27-base DNA aptamer for adenosine-5′-triphosphate (ATP) as a suitable probe for the ATP biosensor based on firefly luciferase activity and AuNPs. Due to ATP application in the firefly luciferase reaction, the increase in luciferase activity and improved detection limits may indicate more stability or accessibility of ATP in the presence of nanoparticles. The bioluminescence intensity increased with the ATP concentration up to 600 µM with a detection limit of 5 µM for ATP. Full article
(This article belongs to the Special Issue Bioluminescent Biosensors for Biomedical Applications)
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Graphical abstract

13 pages, 1509 KiB  
Article
A Luciferase Mutant with Improved Brightness and Stability for Whole-Cell Bioluminescent Biosensors and In Vitro Biosensing
by
Maria Maddalena Calabretta
,
Denise Gregucci
,
Héctor Martínez-Pérez-Cejuela
and
Elisa Michelini
Biosensors 2022, 12(9), 742; https://doi.org/10.3390/bios12090742 - 09 Sep 2022
Cited by 10 | Viewed by 3044
Abstract
The availability of new bioluminescent proteins with tuned properties, both in terms of emission wavelength, kinetics and protein stability, is highly valuable in the bioanalytical field, with the potential to improve the sensitivity and analytical performance of the currently used methods for ATP [...] Read more.
The availability of new bioluminescent proteins with tuned properties, both in terms of emission wavelength, kinetics and protein stability, is highly valuable in the bioanalytical field, with the potential to improve the sensitivity and analytical performance of the currently used methods for ATP detection, whole-cell biosensors, and viability assays among others. We present a new luciferase mutant, called BgLuc, suitable for developing whole-cell biosensors and in vitro biosensors characterized by a bioluminescence maximum of 548 nm, narrow emission bandwidth, favorable kinetic properties, and excellent pH- and thermo-stabilities at 37 and 45 °C and pH from 5.0 to 8.0. We assessed the suitability of this new luciferase for whole-cell biosensing with a cell-based bioreporter assay for Nuclear Factor-kappa B (NF-kB) signal transduction pathway using 2D and 3D human embryonic kidney (HEK293T) cells, and for ATP detection with the purified enzyme. In both cases the luciferase showed suitable for sensitive detection of the target analytes, with better or similar performance than the commercial counterparts. Full article
(This article belongs to the Special Issue Bioluminescent Biosensors for Biomedical Applications)
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