Application of Graphene-Based Sensors in Biomolecular Detection

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (20 December 2023) | Viewed by 1689

Special Issue Editors

Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy
Interests: stem cells; tissue engineering; bioreactors; biosensors; potency assay
Faculty of Medical Engineering, University Politehnica of Bucharest, 011061 Bucharest, Romania
Interests: graphene based biomaterials; multifunctional polymer/graphene materials; graphene based novel architectures for biomedical applications; processing graphene based biomaterials; modification of graphene based biomaterials; graphene based materials characterization; in vivo assessment of graphene based materials; computer aided design of graphene based materials
Special Issues, Collections and Topics in MDPI journals
Faculty of Engineering and Design, University of Bath, Bath BA2 7AY, UK
Interests: lab-on-chip devices for biomedical diagnostics; bioMEMs low-cost integration technologies; electrochemical biosensors; printed bioelectronics; large area electronics
Special Issues, Collections and Topics in MDPI journals
Faculty of Engineering and Design, University of Bath, Bath BA2 7AY, UK
Interests: lab-on-chip; printed-circuit-boards; microfluidics; biosensors; graphene

Special Issue Information

Dear Colleagues,

The extraordinary scope for the application of graphene-based sensors in biomolecular detection is fostering rapid growth of a technology with great potential. However, there are still many challenges to overcome before graphene-based sensors can be routinely used in the field. Beneficial attributes of graphene-based sensing include a very high surface area for analyte capture, favourable semiconductor properties, transparency, and versatile functionalization possibilities for nanobiocomposite fabrication. To ensure such advantages, a high quality of production of functionalized forms of graphene must be maintained so that analytical performance is not compromised. In this Special Issue, we aim to provide an overview of the current state of the art in graphene-based sensor technology and to highlight some of the challenges that need to be addressed. We also invite authors to submit original research articles and opinion and review articles on all aspects of graphene-based sensor technology, including but not limited to sensor design, fabrication, characterization, and applications.

Dr. Jorge S. Burns
Dr. Mariana Ionita
Dr. Despina Moschou
Dr. Sotirios Papamatthaiou
Guest Editors

Manuscript Submission Information

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Keywords

  • biomolecular detection
  • graphene functionalization
  • sensors
  • applications

Published Papers (2 papers)

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Research

11 pages, 2560 KiB  
Communication
Electrochemical DNA Cleavage Sensing for EcoRV Activity and Inhibition with an ERGO Electrode
Biosensors 2024, 14(2), 73; https://doi.org/10.3390/bios14020073 - 30 Jan 2024
Viewed by 408
Abstract
An electrochemically reduced graphene oxide (ERGO) electrode-based electrochemical assay was developed for rapid, sensitive, and straightforward analysis of both activity and inhibition of the endonuclease EcoRV. The procedure uses a DNA substrate designed for EcoRV, featuring a double-stranded DNA (dsDNA) region labeled with [...] Read more.
An electrochemically reduced graphene oxide (ERGO) electrode-based electrochemical assay was developed for rapid, sensitive, and straightforward analysis of both activity and inhibition of the endonuclease EcoRV. The procedure uses a DNA substrate designed for EcoRV, featuring a double-stranded DNA (dsDNA) region labeled with methylene blue (MB) and a single-stranded DNA (ssDNA) region immobilized on the ERGO surface. The ERGO electrode, immobilized with the DNA substrate, was subsequently exposed to a sample containing EcoRV. Upon enzymatic hydrolysis, the cleaved dsDNA fragments were detached from the ERGO surface, leading to a decrease in the MB concentration near the electrode. This diminished the electron transfer efficiency for MB reduction, resulting in a decreased reduction current. This assay demonstrates excellent specificity and high sensitivity, with a limit of detection (LOD) of 9.5 × 10−3 U mL−1. Importantly, it can also measure EcoRV activity in the presence of aurintricarboxylic acid, a known inhibitor, highlighting its potential for drug discovery and clinical diagnostic applications. Full article
(This article belongs to the Special Issue Application of Graphene-Based Sensors in Biomolecular Detection)
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14 pages, 7797 KiB  
Article
Label-Free DNA Biosensor Based on Reduced Graphene Oxide and Gold Nanoparticles
Biosensors 2023, 13(8), 797; https://doi.org/10.3390/bios13080797 - 08 Aug 2023
Viewed by 839
Abstract
Currently available DNA detection techniques frequently require compromises between simplicity, speed, accuracy, and cost. Here, we propose a simple, label-free, and cost-effective DNA detection platform developed at screen-printed carbon electrodes (SPCEs) modified with reduced graphene oxide (RGO) and gold nanoparticles (AuNPs). The preparation [...] Read more.
Currently available DNA detection techniques frequently require compromises between simplicity, speed, accuracy, and cost. Here, we propose a simple, label-free, and cost-effective DNA detection platform developed at screen-printed carbon electrodes (SPCEs) modified with reduced graphene oxide (RGO) and gold nanoparticles (AuNPs). The preparation of the detection platform involved a two-step electrochemical procedure based on GO reduction onto SPCEs followed by the electrochemical reduction of HAuCl4 to facilitate the post-grafting reaction with AuNPs. The final sensor was fabricated by the simple physical adsorption of a single-stranded DNA (ssDNA) probe onto a AuNPs–RGO/SPCE electrode. Each preparation step was confirmed by morphological and structural characterization using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy, respectively. Furthermore, the electrochemical properties of the modified electrodes have been investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results demonstrated that the introduction of AuNPs onto RGO/SPCEs led to an enhancement in surface conductivity, a characteristic that favored an increased sensitivity in detection. The detection process relied on the change in the electrochemical signal induced by the binding of target DNA to the bioreceptor and was particularly monitored by the change in the charge transfer resistance of a [Fe(CN)6]4–/3– redox couple added in the test solution. Full article
(This article belongs to the Special Issue Application of Graphene-Based Sensors in Biomolecular Detection)
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