Special Issue "Recent Advances in Aptasensors for Clinical, Environmental and Food Safety Analysis"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Environmental Biosensors and Biosensing".

Deadline for manuscript submissions: 31 January 2024 | Viewed by 957

Special Issue Editors

1. Department of Molecular Sciences and Nanosystems, Ca’ Foscari University of Venice, Via Torino 155, 30172 Venice, Italy
2. Department of Pharmacy, University of Naples “Federico II”, Via Domenico Montesano, 49, 80131 Napoli, Itatly
Interests: impedimetric; electrochemicaluminescence-based and optical biosensors; characterization of ligand/biomolecule interactions; point-of-care tests; portable analytical devices
Food Quality and Safety Group, International Iberian Nanotechnology Laboratory (INL), Av. Mestre José Veiga, 4715-330 Braga, Portugal
Interests: surface functionalization; electrochemical biosensors; assay development; new materials for biosensor development
Special Issues, Collections and Topics in MDPI journals
Dr. László Kékedy-Nagy
E-Mail Website
Guest Editor
1. Department of Electrical and Computer Engineering, Concordia University, 1455 de Maisonneuve Blvd West, Montreal, QC, Canada
2. Centre for Applied Synthetic Biology, Concordia University, 7141 Sherbrooke St. West, Montreal, QC, Canada
Interests: electrochemical biosensors; point-of-care testing; microfluidic ‘lab-on-chip’ devices; nutrient recycling; water resource sustainability; water electrolysis

Special Issue Information

Dear Colleagues,

Aptamer-based biosensors have garnered considerable attention in recent years thanks to their potential as versatile and sensitive tools for the detection of a wide range of targets, including biomolecules, pathogens and chemicals. The core principle behind aptamer-based biosensors is the highly specific binding affinity between an aptamer and its target molecule. The use of biotechnology and nanotechnology has paved the way for the development of aptamers with specific molecular recognition ability, which can be used in fabricating various biosensors to provide highly sensitive and selective responses.

Compared to traditional biosensors that rely on enzymes or antibodies for target recognition, aptamer-based biosensors have several advantages. Firstly, aptamers can be obtained through an in vitro selection process called the systematic evolution of ligands by exponential enrichment (SELEX), which allows for high affinity and specificity towards target molecules. Moreover, aptamers can be engineered to have desired properties such as stability and selectivity by modifying their sequence or structure. Additionally, compared to antibody-based biosensors, aptamer-based biosensors offer higher productivity, as they can be easily synthesized in vitro. Overall, aptamer-based biosensors have emerged as a promising technology for various applications such as point-of-care testing, clinical diagnostics, food safety monitoring and environmental analysis. In this Special Issue, we aim to collect not only the many successful examples of aptasensors, but also studies that investigate the analytical limitations of such sensing platforms.

Dr. Giulia Moro
Dr. Rui Campos
Dr. László Kékedy-Nagy
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • DNA
  • RNA
  • aptamer
  • small molecule
  • protein
  • diagnostic
  • prognostic
  • biomarkers

Published Papers (1 paper)

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12 pages, 3092 KiB  
Development of a Novel H-Shaped Electrochemical Aptasensor for Detection of Hg2+ Based on Graphene Aerogels–Au Nanoparticles Composite
Biosensors 2023, 13(10), 932; https://doi.org/10.3390/bios13100932 - 18 Oct 2023
Viewed by 821
Hg2+, a highly toxic heavy metal, poses significant environmental and health risks, necessitating rapid detection methods. In this study, we employed an electrochemical aptasensor for rapid and sensitive detection of Hg2+ based on DNA strands (H2 and H3) immobilized graphene [...] Read more.
Hg2+, a highly toxic heavy metal, poses significant environmental and health risks, necessitating rapid detection methods. In this study, we employed an electrochemical aptasensor for rapid and sensitive detection of Hg2+ based on DNA strands (H2 and H3) immobilized graphene aerogels-Au nanoparticles (GAs-AuNPs) hybrid recognition interface and exonuclease III (Exo III)-mediated cyclic amplification. Firstly, Gas-AuNPs were modified on the surface of the ITO electrode to form a sensing interface to increase DNA loading and accelerate electron transfer. Then, DNA helper was generated with the addition of Hg2+ via Exo III-mediated cycling. Finally, the hairpin structures of H2 and H3 were opened with the DNA helper, and then the methylene blue (MB) functionalized DNA (A1 and A2) combined with the H2 and H3 to form an H-shaped structure. The current response of MB as an electrochemical probe was proportional to the concentration of Hg2+. Under optimal conditions, the aptasensor showed excellent performance for Hg2+, achieving a linear range from 1 fM to 10 nM and a detection limit of 0.16 fM. Furthermore, the aptasensor was used to detect Hg2+ in spiked milk samples, achieving a high recovery rate and demonstrating promising application prospects. Full article
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