Electrochemiluminescence Biosensors for Imaging or Detection of Biomarkers, Virus, Bacteria, and Metal Ions

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Environmental Biosensors and Biosensing".

Deadline for manuscript submissions: 26 April 2024 | Viewed by 8431

Special Issue Editor


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Guest Editor
Department of Chemistry, College of Science, Nanjing Agricultural University, Nanjing 210095, China
Interests: electrochemistry; biosensor; electrochemiliminescence; microfluidic chip; amplification strategy

Special Issue Information

Dear Colleagues,

Electrochemiluminescence biosensor is an important analytical device which combines the advantages of highly sensitive electrochemiluminescence techniques and the selectivity of biological recognition events. The designation of portable electrochemiluminescence biosensors for sensitive, selective, and accurate determination of biomarkers, viruses, bacteria, and metal ions has attracted significant attention in recent years. Various amplification strategies, such as nanotechnology, DNA-amplification methods, and enzyme-assisted processes, have been studied and integrated in electrochemiluminescence biosensors to achieve sensitive measurement. Furthermore, by coupling microfabrication technology, these biosensors can realize high-throughput determination through screening the visual electrochemiluminescence signal on the electrode array, making this an effective approach for point-of-care diagnosis.

Therefore, this Special Issue covers the research area of designation of versatile electrochemiluminescence biosensors and their applications in food control, the environmental area, and disease diagnosis.

Dr. Meisheng Wu
Guest Editor

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Keywords

  • food safety
  • early disease diagnosis
  • point of care
  • electrochemiluminescence
  • visual assay
  • high-throughput
  • electrode array

Published Papers (6 papers)

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Research

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13 pages, 3640 KiB  
Article
Methylene-Blue-Encapsulated Metal-Organic-Framework-Based Electrochemical POCT Platform for Multiple Detection of Heavy Metal Ions in Milk
by Huiwen Xiong, Pintao Li, Fei Cun, Hui Chen and Jilie Kong
Biosensors 2023, 13(8), 783; https://doi.org/10.3390/bios13080783 - 02 Aug 2023
Cited by 2 | Viewed by 1019
Abstract
Considering the high risk of heavy metal ions (HMIs) transferring through the food chain and accumulating in milk, a flexible and facile point-of-care testing (POCT) platform is urgently needed for the accurate, sensitive, and highly selective on-site quantification of multiple HMIs in milk. [...] Read more.
Considering the high risk of heavy metal ions (HMIs) transferring through the food chain and accumulating in milk, a flexible and facile point-of-care testing (POCT) platform is urgently needed for the accurate, sensitive, and highly selective on-site quantification of multiple HMIs in milk. In this work, a cost-effective disk with six screen-printed electrodes (SPEs) was designed for hand-held electrochemical detection. Metal organic frameworks (MOFs) were adopted to amplify and enhance the electrochemical signals of methylene blue (MB). Using differential pulse voltammetry (DPV) methods, low limits of detection for four HMIs (Cd2+, 0.039 ppb; Hg2+, 0.039 ppb; Pb2+, 0.073 ppb; and As3+, 0.022 ppb) were achieved within four minutes. Moreover, the quantitative POCT system was applied to milk samples. The advantages of low cost, ease of on-site implementation, fast response, and accuracy allow for the POCT platform to be used in practical monitoring applications for the quantitation of multiple HMIs in milk samples. Full article
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20 pages, 6842 KiB  
Article
Kinetic Determination of Acetylsalicylic Acid Using a CdTe/AgInS2 Photoluminescence Probe and Different Chemometric Models
by Rafael C. Castro, Ricardo N. M. J. Páscoa, M. Lúcia M. F. S. Saraiva, João L. M. Santos and David S. M. Ribeiro
Biosensors 2023, 13(4), 437; https://doi.org/10.3390/bios13040437 - 30 Mar 2023
Cited by 1 | Viewed by 1071
Abstract
The combination of multiple quantum dots (QDs) in a multi-emitter nanoprobe can be envisaged as a promising sensing scheme, as it enables obtaining a collective response of individual emitters towards a given analyte and allows for achieving specific analyte-response profiles. The processing of [...] Read more.
The combination of multiple quantum dots (QDs) in a multi-emitter nanoprobe can be envisaged as a promising sensing scheme, as it enables obtaining a collective response of individual emitters towards a given analyte and allows for achieving specific analyte-response profiles. The processing of these profiles using adequate chemometric methods empowers a more sensitive, reliable and selective determination of the target analyte. In this work, we developed a kinetic fluorometric method consisting of a dual CdTe/AgInS2 quantum dots photoluminescence probe for the determination of acetylsalicylic acid (ASA). The fluorometric response was acquired as second-order time-based excitation/emission matrices that were subsequently processed using chemometric methods seeking to assure the second-order advantage. The data obtained in this work are considered second-order data as they have a three-dimensional size, I × J × K (where I represents the samples’ number, J the fluorescence emission wavelength while K represents the time). In order to select the most adequate chemometric method regarding the obtained data structure, different chemometric models were tested, namely unfolded partial least squares (U-PLS), N-way partial least squares (N-PLS), multilayer feed-forward neural networks (MLF-NNs) and radial basis function neural networks (RBF-NNs). Full article
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11 pages, 2028 KiB  
Article
Sandwich-Type Electrochemiluminescence Immunosensor Based on CDs@dSiO2 Nanoparticles as Nanoprobe and Co-Reactant
by A-Ling Chen, Xiao-Yan Wang, Qing Zhang, Ning Bao and Shou-Nian Ding
Biosensors 2023, 13(1), 133; https://doi.org/10.3390/bios13010133 - 13 Jan 2023
Cited by 4 | Viewed by 1687
Abstract
In general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CDs@dSiO2 NPs) to form [...] Read more.
In general, co-reactants are essential in highly efficient electrochemiluminescence (ECL) systems. Traditional co-reactants are usually toxic, so it is necessary to develop new environmentally friendly co-reactants. In this work, carbon dots (CDs) were assembled with dendritic silica nanospheres (CDs@dSiO2 NPs) to form a co-reactant of Ru(bpy)32+. Subsequently, a sandwich immunosensor for detecting human chorionic gonadotropin (HCG) was constructed based on CDs@dSiO2 NPs as co-reactants, the nanoprobe loaded with the secondary antibody, and Ru(bpy)32+ as a luminophore. In addition, compared to directly as a signal probe, the luminophore Ru (bpy)32+ as a part of the electrolyte solution is simpler in this work. The immunosensor has an extremely low limit of detection of 0.00019 mIU/mL. This work describes the synthesis of low-toxic, efficient, and environmentally friendly CDs, which have become ideal co-reactants of Ru(bpy)32+, and proposes an ECL immunosensor with excellent stability and selectivity, which has great potential in clinical applications. Full article
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9 pages, 2436 KiB  
Article
Electrochemiluminescence Aptasensor Based on Gd(OH)3 Nanocrystalline for Ochratoxin A Detection in Food Samples
by Chunyuan Tian, Minggang Wei, Xiaobin Wang, Qing Hua, Feiyan Tang, Lijun Zhao, Xuming Zhuang and Feng Luan
Biosensors 2022, 12(12), 1141; https://doi.org/10.3390/bios12121141 - 07 Dec 2022
Cited by 1 | Viewed by 1341
Abstract
In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)3 nanocrystals with K2S2O8 as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of [...] Read more.
In the present study, the electrochemiluminescence (ECL) properties of Gd(OH)3 nanocrystals with K2S2O8 as the cathode coreactant were studied for the first time. Based on the prominent ECL behavior of this material and the excellent specificity of the aptamer technique, an ECL aptasensor for the detection of ochratoxin A (OTA) was formulated successfully. Over an OTA concentration range of 0.01 pg mL−1 to 10 ng mL−1, the change in the ECL signal was highly linear with the OTA concentration, and the limit of detection (LOD) was 0.0027 pg mL−1. Finally, the ECL aptasensor was further used to detect OTA in real samples (grapes and corn) and satisfactory results were obtained, which indicated that the built method is expected to be applied in food detection. Full article
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Review

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25 pages, 4818 KiB  
Review
Engineered Electrochemiluminescence Biosensors for Monitoring Heavy Metal Ions: Current Status and Prospects
by Yuanyuan Chen, Hui Jiang, Xiaohui Liu and Xuemei Wang
Biosensors 2024, 14(1), 9; https://doi.org/10.3390/bios14010009 - 22 Dec 2023
Viewed by 1275
Abstract
Metal ion contamination has serious impacts on environmental and biological health, so it is crucial to effectively monitor the levels of these metal ions. With the continuous progression of optoelectronic nanotechnology and biometrics, the emerging electrochemiluminescence (ECL) biosensing technology has not only proven [...] Read more.
Metal ion contamination has serious impacts on environmental and biological health, so it is crucial to effectively monitor the levels of these metal ions. With the continuous progression of optoelectronic nanotechnology and biometrics, the emerging electrochemiluminescence (ECL) biosensing technology has not only proven its simplicity, but also showcased its utility and remarkable sensitivity in engineered monitoring of residual heavy metal contaminants. This comprehensive review begins by introducing the composition, advantages, and detection principles of ECL biosensors, and delving into the engineered aspects. Furthermore, it explores two signal amplification methods: biometric element-based strategies (e.g., HCR, RCA, EDC, and CRISPR/Cas) and nanomaterial (NM)-based amplification, including quantum dots, metal nanoclusters, carbon-based nanomaterials, and porous nanomaterials. Ultimately, this review envisions future research trends and engineered technological enhancements of ECL biosensors to meet the surging demand for metal ion monitoring. Full article
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19 pages, 4463 KiB  
Review
Recent Advances in Electrochemiluminescence Biosensors for Mycotoxin Assay
by Longsheng Jin, Weishuai Liu, Ziying Xiao, Haijian Yang, Huihui Yu, Changxun Dong and Meisheng Wu
Biosensors 2023, 13(6), 653; https://doi.org/10.3390/bios13060653 - 14 Jun 2023
Cited by 2 | Viewed by 1416
Abstract
Rapid and efficient detection of mycotoxins is of great significance in the field of food safety. In this review, several traditional and commercial detection methods are introduced, such as high-performance liquid chromatography (HPLC), liquid chromatography/mass spectrometry (LC/MS), enzyme-linked immunosorbent assay (ELISA), test strips, [...] Read more.
Rapid and efficient detection of mycotoxins is of great significance in the field of food safety. In this review, several traditional and commercial detection methods are introduced, such as high-performance liquid chromatography (HPLC), liquid chromatography/mass spectrometry (LC/MS), enzyme-linked immunosorbent assay (ELISA), test strips, etc. Electrochemiluminescence (ECL) biosensors have the advantages of high sensitivity and specificity. The use of ECL biosensors for mycotoxins detection has attracted great attention. According to the recognition mechanisms, ECL biosensors are mainly divided into antibody-based, aptamer-based, and molecular imprinting techniques. In this review, we focus on the recent effects towards the designation of diverse ECL biosensors in mycotoxins assay, mainly including their amplification strategies and working mechanism. Full article
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