Micro/Nanofluidics for Single-Cell Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 1894

Special Issue Editors

Department of Biomedical Engineering, Beihang University, Beijing, China
Interests: single cell nanoengineering; biosensors and POCT devices; wearable flexible biosensors
School of Engineering Medicine, Beihang University, Beijing, China
Interests: single cell nanoengineering; biosensors and POCT devices; wearable flexible biosensors

Special Issue Information

Dear Colleagues,

Different cells show obvious heterogeneity in genetic and phenotypic properties that can differentially promote disease progression, tumor metastasis and drug resistance. Emerging single-cell analysis technologies open up a new avenue to profile individual cells and investigate what roles they play in these processes. Traditional single-cell analysis methods such as single-cell sequencing are mainly limited by cytogenetic analysis, and face the problems of low throughput, complex steps, and high price. Micro/nanofluidics (e.g., microfluidic chips) have the ability to pioneer the field of single-cell analysis, enabling multiple analysis of gene, protein, and cell behavior through accurate cell manipulation, the construction of on-chip biomimetic scenarios, etc. Meanwhile, the flexible structure design and convenient standardized preparation process of micro/nanofluidic devices bring distinct advantages for single-cell analysis, such as high throughput, high efficiency, high sensitivity, and low cost.

This Special Issue is soliciting original research articles and reviews on “Micro/Nanofluidics for Single-Cell Analysis”, mainly focusing on novel techniques and methods based on micro/nanofluidics, as well as corresponding new findings at the single-cell level. We are looking forward to your submissions.

Prof. Dr. Lingqian Chang
Dr. Zaizai Dong
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • micro/nanofluidics
  • single-cell analysis
  • micro/nanofluidic chips
  • biochips
  • lab-on-a-chip
  • cell behavior
  • cell heterogeneity

Published Papers (1 paper)

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Review

19 pages, 5861 KiB  
Review
Electrical Characterization and Analysis of Single Cells and Related Applications
by Weitao Zhu, Jiaao Wang, Hongzhi Luo, Binwen Luo, Xue Li, Shan Liu and Chenzhong Li
Biosensors 2023, 13(10), 907; https://doi.org/10.3390/bios13100907 - 26 Sep 2023
Cited by 3 | Viewed by 1556
Abstract
Biological parameters extracted from electrical signals from various body parts have been used for many years to analyze the human body and its behavior. In addition, electrical signals from cancer cell lines, normal cells, and viruses, among others, have been widely used for [...] Read more.
Biological parameters extracted from electrical signals from various body parts have been used for many years to analyze the human body and its behavior. In addition, electrical signals from cancer cell lines, normal cells, and viruses, among others, have been widely used for the detection of various diseases. Single-cell parameters such as cell and cytoplasmic conductivity, relaxation frequency, and membrane capacitance are important. There are many techniques available to characterize biomaterials, such as nanotechnology, microstrip cavity resonance measurement, etc. This article reviews single-cell isolation and sorting techniques, such as the micropipette separation method, separation and sorting system (dual electrophoretic array system), DEPArray sorting system (dielectrophoretic array system), cell selector sorting system, and microfluidic and valve devices, and discusses their respective advantages and disadvantages. Furthermore, it summarizes common single-cell electrical manipulations, such as single-cell amperometry (SCA), electrical impedance sensing (EIS), impedance flow cytometry (IFC), cell-based electrical impedance (CEI), microelectromechanical systems (MEMS), and integrated microelectrode array (IMA). The article also enumerates the application and significance of single-cell electrochemical analysis from the perspectives of CTC liquid biopsy, recombinant adenovirus, tumor cells like lung cancer DTCs (LC-DTCs), and single-cell metabolomics analysis. The paper concludes with a discussion of the current limitations faced by single-cell analysis techniques along with future directions and potential application scenarios. Full article
(This article belongs to the Special Issue Micro/Nanofluidics for Single-Cell Analysis)
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