Biosensors for Rapid, High-Throughput and Sensitive Detection of Pathogens in Environmental, Food and Clinical Testing

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Environmental Biosensors and Biosensing".

Deadline for manuscript submissions: closed (1 September 2023) | Viewed by 3463

Special Issue Editor

Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China
Interests: biosensors; POCT methods; foodborne pathogens detection

Special Issue Information

Dear Colleagues,

Pathogens are the main concern in food safety, clinical monitoring and environmental protection. The main common pathogens include Salmonella, Vibrio parahaemolyticus, Bacillus cereus, Escherichia coli and so on. Screening of these pathogens is key to ensure food, clinical and environmental safety. However, the development of assays and instruments which can shorten the detection time is required, thereby providing an early warning of pathogen risks. Biosensors have been applied in the rapid detection of pathogenic bacteria, disease diagnosis, imaging research and so on. Nevertheless, there is a growing demand for food-borne pathogen biosensors or biochips that are robust, easy to use and, more importantly, mass-producible. This Special Issue entitled "Biosensors for Rapid, High-throughput and Sensitive Detection of Pathogens in Environmental, Food and Clinical Testing" focuses on the construction of phages, antibodies or nucleic acid probe-based biosensors, together with their recent advances in the detection and application of food-borne pathogens. We invite submissions of research that will help to advance the field of biosensor technology and its application for high-throughput, rapid and sensitive analysis of pathogens.

Prof. Dr. Ning Gan
Guest Editor

Manuscript Submission Information

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Published Papers (2 papers)

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Research

9 pages, 2198 KiB  
Communication
Detection of Micrometer-Sized Virus Aerosols by Using a Real-Time Bioaerosol Monitoring System
by Hyunsoo Seo, Young-Su Jeong, Jaekyung Bae, Kibong Choi and Moon-Hyeong Seo
Biosensors 2024, 14(1), 27; https://doi.org/10.3390/bios14010027 - 02 Jan 2024
Cited by 1 | Viewed by 1180
Abstract
This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, and [...] Read more.
This study investigates a real-time handheld bioaerosol monitoring system for the detection of biological particles using UV-LED and light-induced fluorescence technology. Biological particles produce both scattering and fluorescence signals simultaneously, which can help distinguish them from general particles. The detected scattering, fluorescence, and simultaneous signals are then converted into photon signals and categorized based on predetermined criteria. A reliable biological particle generator was required to validate the performance of the system. This study explores the use of an M13 bacteriophage as a virus simulant of biological agents and employs a customized inkjet aerosol generator to produce M13 bacteriophage aerosols of a specific size by controlling the concentration of M13. We confirmed that micro-sized, narrowly dispersed M13 aerosols were efficiently generated. Additionally, we confirmed the performance of this real-time handheld bioaerosol monitoring system by detecting viruses. Full article
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15 pages, 5282 KiB  
Article
Dual-Mode Biosensor for Simultaneous and Rapid Detection of Live and Whole Salmonella typhimurium Based on Bioluminescence and Fluorescence Detection
by Zhenli Xu, Bailu Liu, Dengfeng Li, Zhenzhong Yu and Ning Gan
Biosensors 2023, 13(3), 401; https://doi.org/10.3390/bios13030401 - 19 Mar 2023
Cited by 2 | Viewed by 1791
Abstract
Both live and dead Salmonella typhimurium (S.T) are harmful to human health, but there are differences in pathological mechanism, dosage, and security. It is crucial to develop a rapid and simultaneous assay to distinguish and quantify live and dead S.T in foods. Herein, [...] Read more.
Both live and dead Salmonella typhimurium (S.T) are harmful to human health, but there are differences in pathological mechanism, dosage, and security. It is crucial to develop a rapid and simultaneous assay to distinguish and quantify live and dead S.T in foods. Herein, one dual-mode biosensor for simultaneous detection of live and dead S.T was fabricated based on two phage probes, using portable bioluminescence and fluorescent meter as detectors, respectively. Firstly, a magnetic phage capture probe (M-P1) and a phage signal tag (P2-S) labeled with SYTO 13 fluorescent dye were prepared, respectively. Both M-P1 and P2-S can specifically conjugate with S.T to form a magnetic sandwich complex. After magnetic separation, the isolated complex can emit a fluorescent signal under an excited 365 nm laser, which can reflect the total amount of S.T. Afterwards, the lysozyme was added to decompose the captured live S.T, which can release ATP and produce a bioluminescent signal corresponding to the live S.T amount. The dead S.T concentration can be deduced by the difference between total and live examples. The detection limit of 55 CFU/mL for total S.T and 9 CFU/mL for live ones was within 20 min. The assay was successfully employed in milk samples and prospectively for on-site screening of other dead and live bacteria, while changing the phages for the targets. Full article
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