Special Issue "Advanced Biosensors for Visual Detection"

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 30 April 2024 | Viewed by 1074

Special Issue Editors

Prof. Dr. Heon-Ho Jeong
E-Mail Website
Guest Editor
Department of Chemical and Biomolecular Engineering, Chonnam National University, Yeosu 59626, Republic of Korea
Interests: paper-based analytical device (μPAD); microfluidics; enzymatic reaction; nano- and micro-particle
1. Department of Green Chemical Engineering, Sangmyung University, Cheonan, Chungnam 31066, Republic of Korea
2. Future Environment and Energy Research Institute, Sangmyung University, Cheonan, Chungnam 31066, Republic of Korea
Interests: colorimetric detection; organ-on-a-chip
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Special Issue Information

Dear Colleagues,

The recent developments in the visualization of target molecules have significantly contributed not only to a wide range of scientific applications, including cell biology and monitoring, but also to industry fields, including disease and body fluid diagnosis and chemical sensing in environment, which are related to human life. There are two main fields for advanced biosensors for visual detection, including the development of sensing probes and analytical devices. A recent research trend for advanced sensing probe is the visualization of on-demand single targets from complex components by specifically interacting with the analyte and sensing probe, providing high sensitivity and selectivity. Additionally, a recent trend for advanced analytical devices is the miniaturization of the devices, which enables a low cost, portability, small volume usage, multiple and high-throughput assays, and the improvement of sensitivity and selectivity.

The aim of this Special Issue is to highlight the recent advances in sensing probes and devices. We invite experts engaged in various fields of biosensing, including colorimetric assay based on organic and inorganic materials, visualization via chemical and physical approaches, optoelectronic nose, photonic crystal, optical devices, microfluidic devices, and paper-based analytical devices, among others, to submit their research. Submissions may include original research articles, review articles, and communications. 

Prof. Dr. Heon-Ho Jeong
Dr. Sung-Min Kang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • biosensors
  • microfluidic paper-based analytical device (μPAD)
  • colorimetric detection
  • optical sensor array
  • cell imaging
  • medical diagnostics
  • environmental sensing

Published Papers (1 paper)

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12 pages, 1885 KiB  
A Facile Method to Fabricate an Enclosed Paper-Based Analytical Device via Double-Sided Patterning for Ionic Contaminant Detection
Biosensors 2023, 13(10), 915; https://doi.org/10.3390/bios13100915 - 05 Oct 2023
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Microfluidic paper-based analytical devices (μPADs) have been developed for use in a variety of diagnosis and analysis fields. However, conventional μPADs with an open-channel system have limitations for application as analytical platforms mainly because of the evaporation and contamination of the sample solution. [...] Read more.
Microfluidic paper-based analytical devices (μPADs) have been developed for use in a variety of diagnosis and analysis fields. However, conventional μPADs with an open-channel system have limitations for application as analytical platforms mainly because of the evaporation and contamination of the sample solution. This study demonstrates the design and fabrication of an enclosed three-dimensional(3D)-μPAD and its application as a primary early analysis platform for ionic contaminants. To generate the hydrophobic PDMS barrier, double-sided patterning is carried out using a PDMS blade-coated stamp mold that is fabricated using 3D printing. The selective PDMS patterning can be achieved with controlled PDMS permeation of the cellulose substrate using 3D-designed stamp molds. We find the optimal conditions enabling the formation of enclosed channels, including round shape pattern and inter-pattern distance of 10 mm of stamp design, contact time of 0.5 min, and spacer height of 300 µm of double-sided patterning procedure. As a proof of concept, this enclosed 3D-μPAD is used for the simultaneous colorimetric detection of heavy metal ions in a concentration range of 0.1–2000 ppm, including nickel (Ni2+), copper (Cu2+), mercury (Hg2+), and radioactive isotope cesium-137 ions (Cs+). We confirm that qualitative analysis and image-based quantitative analysis with high reliability are possible through rapid color changes within 3 min. The limits of detection (LOD) for 0.55 ppm of Ni2+, 5.05 ppm of Cu2+, 0.188 ppm of Hg2+, and 0.016 ppm of Cs+ are observed, respectively. In addition, we confirm that the analysis is highly reliable in a wide range of ion concentrations with CV values below 3% for Ni2+ (0.56%), Cu2+ (0.45%), Hg2+ (1.35%), and Cs+ (2.18%). This method could be a promising technique to develop a 3D-μPAD with various applications as a primary early analysis device in the environmental and biological industries. Full article
(This article belongs to the Special Issue Advanced Biosensors for Visual Detection)
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