Protein Engineering: The Present and the Future 2.0

A special issue of Biophysica (ISSN 2673-4125).

Deadline for manuscript submissions: closed (30 November 2023) | Viewed by 3073

Special Issue Editors


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Guest Editor
Department of Biochemistry and Molecular and Cell Biology, Institute for Biocomputation and Physics of Complex Systems (BIFI), University of Zaragoza, 50009 Zaragoza, Spain
Interests: protein stability; protein engineering; protein folding; biocomputation; drug discovery
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Guest Editor
Biochemical Sciences "Alessandro Rossi Fanelli", Sapienza Università di Roma, 00185 Rome, Italy
Interests: metabolic reprogramming in cancer cells ageing and neural diseases; metabolism of small dinucleotides involved in the formation of bacterial biofilm in chronic infections; role of nitric oxide and redox metabolism in bacterial pathogens and eukaryotic cells
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Proteins are simple polymers with extraordinary properties of enormous biological and economic value. They are widely used in biological catalysis, as key components of analytical methods, or as highly specific drugs for personalized medicine. Protein engineering uses synthetic methods that allow the application of rational physicochemical knowledge and the power of evolutionary approaches to the goal of creating, in useful quantities, novel proteins that exhibit advantageous properties. In some cases, the challenge is to stabilize a natural protein for cheaper production, easier transport and storage, and longer operational life. In others, completely new properties are sought, which requires a greater amount of design. Significant advances in the understanding of protein energetics, in computational methods for sequence and structural analysis, and in synthetic methods, combined with growing economic and social interest in proteins, claim the logical transformation of Protein Engineering into a predictive quantitative discipline, where success is guaranteed by good design. In this Special Issue we will show, with examples of their application to specific proteins, the most advanced methods that anticipate the transformation of Protein Engineering from an art for practitioners to a reliable technology.

Prof. Dr. Javier Sancho
Prof. Dr. Francesca Cutruzzola
Guest Editors

Manuscript Submission Information

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Keywords

  • protein design
  • computational methods
  • evolutionary methods
  • protein stabilization
  • protein tailoring
  • industrial proteins
  • medicinal proteins

Published Papers (1 paper)

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Research

16 pages, 7177 KiB  
Article
Adsorption of Heparin-Binding Fragments of Fibronectin onto Hydrophobic Surfaces
by Viswanath Vittaladevaram and David L. Cheung
Biophysica 2023, 3(3), 409-424; https://doi.org/10.3390/biophysica3030027 - 23 Jun 2023
Viewed by 2538
Abstract
Fibronectin is a multi-domain, extracellular matrix protein that plays a number of biological roles. As the adsorption of fibronectin onto the surface of implanted devices can lead to an inflammatory response or bacterial colonisation, understanding the interaction of fibronectin with material surfaces is [...] Read more.
Fibronectin is a multi-domain, extracellular matrix protein that plays a number of biological roles. As the adsorption of fibronectin onto the surface of implanted devices can lead to an inflammatory response or bacterial colonisation, understanding the interaction of fibronectin with material surfaces is important in the design of materials for biomedical applications. This, however, relies on having knowledge of the molecular-scale behaviour of proteins, which is difficult to investigate experimentally. In this paper, we used molecular dynamics simulations to investigate the adsorption of heparin-binding fibronectin domains onto hydrophobic surfaces. Despite the high similarity between these, their adsorption differs both in terms of the strength and the specificity of this, indicating that relatively small changes in protein structure can lead to significant changes in adsorption behaviour. This suggests that the interplay between protein structure and surface chemistry is vital for understanding the protein adsorption process and the design of novel biomaterials. Full article
(This article belongs to the Special Issue Protein Engineering: The Present and the Future 2.0)
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