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Biomolecules
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Contributions of Promoters to the Physiology, Biochemistry and Molecular Biology...
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Contributions of Promoters to the Physiology, Biochemistry and Molecular Biology Processes of Insects

A special issue of Biomolecules (ISSN 2218-273X).

Deadline for manuscript submissions: closed (31 December 2022) | Viewed by 6572

Special Issue Editor

Prof. Dr. Dov Borovsky

E-Mail Website
Guest Editor
Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz School of Medicine, Aurora, CO, USA
Interests: insect biochemistry and molecular biology; molecular engineering; peptide hormones; biological control of insects; JH and ecdysone biosynthesis; insect receptors; vitellogenesis; starch and glycogen biosynthesis

Special Issue Information

Dear Colleagues,

Promoters are regulatory highly conserved DNA sequences that control gene expressions in bacteria and eukaryotes. In insects, promoters control diverse physiological events that are important for the development and function of insects from Malpighian tubules to salivary glands, hemolymph production to immunity, egg yolk and hormonal production. Many promoters have been used to genetically engineer insects to control disease vectors and agricultural pest insects. In this context, it is necessary to publish a comprehensive collection of research that addresses these issues, and thus we encourage the submission of original papers as well as review articles on the “Contributions of Promoters to the Physiology, Biochemistry and Molecular Biology Processes of Insects”.
We welcome manuscripts from national and international colleagues related to updated, novel approaches and new results on the subject. We invite manuscripts that cover insect promoters dealing with all biological events in insects, including the use of insect promoters in genetic engineering to control agricultural pests and vector-transmitting insects. The manuscripts have a limit of 11,000 words, including figures. Reviews should be limited to the discussion of single promoters, rather than the whole field of insect promoters. 

Prof. Dr. Dov Borovsky
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

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Research

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24 pages, 5103 KiB  
Article
Cloning and Characterization of Drosophila melanogaster Juvenile Hormone Epoxide Hydrolases (JHEH) and Their Promoters
by Dov Borovsky, Hilde Breyssens, Esther Buytaert, Tom Peeters, Carole Laroye, Karolien Stoffels and Pierre Rougé
Biomolecules 2022, 12(7), 991; https://doi.org/10.3390/biom12070991 - 16 Jul 2022
Cited by 3 | Viewed by 2279
Abstract
Juvenile hormone epoxide hydrolase (JHEH) plays an important role in the metabolism of JH III in insects. To study the control of JHEH in female Drosophila melanogaster, JHEH 1, 2 and 3 cDNAs were cloned and sequenced. Northern blot analyses showed that [...] Read more.
Juvenile hormone epoxide hydrolase (JHEH) plays an important role in the metabolism of JH III in insects. To study the control of JHEH in female Drosophila melanogaster, JHEH 1, 2 and 3 cDNAs were cloned and sequenced. Northern blot analyses showed that the three transcripts are expressed in the head thorax, the gut, the ovaries and the fat body of females. Molecular modeling shows that the enzyme is a homodimer that binds juvenile hormone III acid (JH IIIA) at the catalytic groove better than JH III. Analyses of the three JHEH promoters and expressing short promoter sequences behind a reporter gene (lacZ) in D. melanogaster cell culture identified a JHEH 3 promoter sequence (626 bp) that is 10- and 25-fold more active than the most active promoter sequences of JHEH 2 and JHEH 1, respectively. A transcription factor (TF) Sp1 that is involved in the activation of JHEH 3 promoter sequence was identified. Knocking down Sp1 using dsRNA inhibited the transcriptional activity of this promoter in transfected D. melanogaster cells and JH III and 20HE downregulated the JHEH 3 promoter. On the other hand, JH IIIA and farnesoic acid did not affect the promoter, indicating that JH IIIA is JHEH’s preferred substrate. A transgenic D. melanogaster expressing a highly activated JHEH 3 promoter behind a lacZ reporter gene showed promoter transcriptional activity in many D. melanogaster tissues. Full article
(This article belongs to the Special Issue Contributions of Promoters to the Physiology, Biochemistry and Molecular Biology Processes of Insects)
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Review

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13 pages, 758 KiB  
Review
Use of Insect Promoters in Genetic Engineering to Control Mosquito-Borne Diseases
by Vanessa Bottino-Rojas and Anthony A. James
Biomolecules 2023, 13(1), 16; https://doi.org/10.3390/biom13010016 - 21 Dec 2022
Cited by 4 | Viewed by 3764
Abstract
Mosquito transgenesis and gene-drive technologies provide the basis for developing promising new tools for vector-borne disease prevention by either suppressing wild mosquito populations or reducing their capacity from transmitting pathogens. Many studies of the regulatory DNA and promoters of genes with robust sex-, [...] Read more.
Mosquito transgenesis and gene-drive technologies provide the basis for developing promising new tools for vector-borne disease prevention by either suppressing wild mosquito populations or reducing their capacity from transmitting pathogens. Many studies of the regulatory DNA and promoters of genes with robust sex-, tissue- and stage-specific expression profiles have supported the development of new tools and strategies that could bring mosquito-borne diseases under control. Although the list of regulatory elements available is significant, only a limited set of those can reliably drive spatial–temporal expression. Here, we review the advances in our ability to express beneficial and other genes in mosquitoes, and highlight the information needed for the development of new mosquito-control and anti-disease strategies. Full article
(This article belongs to the Special Issue Contributions of Promoters to the Physiology, Biochemistry and Molecular Biology Processes of Insects)
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