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Biomolecules
Special Issues
State-of-the-Art on Biochemistry and Molecular Biology in Spain 2021
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State-of-the-Art on Biochemistry and Molecular Biology in Spain 2021

A special issue of Biomolecules (ISSN 2218-273X).

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 2799

Special Issue Editors

Prof. Dr. Jose Lorenzo Rodriguez

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Guest Editor
Centro Tecnológico de la Carne de Galicia, 32900 Orense, Spain
Interests: meat quality; genetic influences in meat quality; genetic improvement
Special Issues, Collections and Topics in MDPI journals
Dr. Vito Verardo

E-Mail Website
Guest Editor
1. Department of Nutrition and Food Science, Campus of Cartuja, University of Granada, 18011 Granada, Spain
2. Institute of Nutrition and Food Technology ‘José Mataix’, Biomedical Research Center, University of Granada, Avda del Conocimiento sn., 18100 Armilla, Granada, Spain
Interests: antioxidants; natural product chemistry; antioxidant activity; phytochemicals; lipids; lipid oxidation
Special Issues, Collections and Topics in MDPI journals
Dr. Adrián Velázquez Campoy

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Guest Editor
1. Institute of Biocomputation and Physics of Complex Systems (BIFI), Universidad de Zaragoza, 50018 Zaragoza, Spain
2. Departamento de Bioquímica y Biología Molecular y Celular, Universidad de Zaragoza, 50009 Zaragoza, Spain
Interests: protein biophysics; protein interactions; protein stability and folding; cooperativity and allostery; biological calorimetry; drug discovery
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The aim of this Special Issue is to provide a comprehensive view of state-of-the-art biochemistry and molecular biology in Spain. We invite research papers that will consolidate our understanding in this area. Potential topics include but are not limited to the following:

  • Biochemical changes through the manufacture of foods;
  • Biological activity of food and plant bioactive compounds;
  • Formation and biological activity of unhealthy compounds in foods;
  • Synthesis and biological activity of new compounds;
  • Formation of healthy and unhealthy compounds during food processing;
  • Relationship between healthy and unhealthy compounds, and health status.

Prof. Dr. Jose Lorenzo Rodriguez
Dr. Vito Verardo
Dr. Adrián Velázquez Campoy
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

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Research

13 pages, 3099 KiB  
Article
Clotrimazole Fluidizes Phospholipid Membranes and Localizes at the Hydrophobic Part near the Polar Part of the Membrane
by Alessio Ausili, Illya Yakymenko, José A. Teruel and Juan C. Gómez-Fernández
Biomolecules 2021, 11(9), 1304; https://doi.org/10.3390/biom11091304 - 02 Sep 2021
Cited by 2 | Viewed by 2079
Abstract
Clotrimazole (1-[(2-chlorophenyl)-diphenylmethyl]-imidazole) is an azole antifungal drug belonging to the imidazole subclass that is widely used in pharmacology and that can be incorporated in membranes. We studied its interaction with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) phospholipid vesicles by using differential scanning calorimetry and found that the [...] Read more.
Clotrimazole (1-[(2-chlorophenyl)-diphenylmethyl]-imidazole) is an azole antifungal drug belonging to the imidazole subclass that is widely used in pharmacology and that can be incorporated in membranes. We studied its interaction with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) phospholipid vesicles by using differential scanning calorimetry and found that the transition temperature decreases progressively as the concentration of clotrimazole increases. However, the temperature of completion of the transition remained constant despite the increase of clotrimazole concentration, suggesting the formation of fluid immiscibility. 1H-NMR and 1H NOESY MAS-NMR were employed to investigate the location of clotrimazole in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) phospholipid membranes. In the presence of clotrimazole, all the resonances originating from POPC were shifted upfield, but mainly those corresponding to C2 and C3 of the fatty acyl, chains suggesting that clotrimazole aromatic rings preferentially locate near these carbons. In the same way, 2D-NOESY measurements showed that the highest cross-relaxation rates between protons of clotrimazole and POPC were with those bound to the C2 and C3 carbons of the fatty acyl chains. Molecular dynamics simulations indicated that clotrimazole is located near the top of the hydrocarbon-chain phase, with the nitrogen atoms of the imidazole ring of clotrimazole being closest to the polar group of the carbonyl moiety. These results are in close agreement with the NMR and the conclusion is that clotrimazole is located near the water–lipid interface and in the upper part of the hydrophobic bilayer. Full article
(This article belongs to the Special Issue State-of-the-Art on Biochemistry and Molecular Biology in Spain 2021)
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