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Biomolecules
Special Issues
Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces
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Special Issue "Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces"

A special issue of Biomolecules (ISSN 2218-273X). This special issue belongs to the section "Biological and Bio- Materials".

Deadline for manuscript submissions: 15 July 2023 | Viewed by 6506

Special Issue Editors

Dr. Monika Wasilewska

E-Mail Website
Guest Editor
Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Science, Niezapominajek 8, 30-239 Cracow, Poland
Interests: protein adsorption; mechanisms of protein adsorption; stability of biocolloidal suspensions; adsorption of biocolloidal particles; streaming potential; biosensors
Special Issues, Collections and Topics in MDPI journals
Dr. Aneta Michna

E-Mail Website
Guest Editor
Institute of Catalysis and Surface Chemistry of the Polish Academy of Sciences, Krakow, Poland
Interests: biocompatible polyelectrolyte films; polysaccharides; biomaterials; application of electrokinetic techniques for determination of the properties of nanocomposites; binding proteins to polyelectrolyte multilayers; determination of the stability of polyelectrolyte mono- and multilayers; determination of the physicochemical properties of polyelectrolytes in bulk
Special Issues, Collections and Topics in MDPI journals
Dr. Maria Morga

E-Mail Website
Guest Editor
Institute of Catalysis and Surface Chemistry of the Polish Academy of Sciences, Krakow, Poland
Interests: preparation and characterization of mono-, bi-, and multilayers of macroions and nanoparticles; mechanisms of macroion and nanoparticle adsorption; kinetics adsorption and desorption of macromolecules and nanoparticles; macroion adsorption at homogeneous and heterogeneous surfaces; stability of monolayers and bilayers composed of macromolecules and nanoparticles; biocompatibility of composed layered materials; electrokinetic potential at solid/liquid interfaces; streaming potential at solid/liquid interfaces; layer by layer deposition of colloidal particles; polypeptides and their monolayers; nanoparticles/macroions composite material; biological applications of macromolecules and nanoparticles
Dr. Andrey G. Cherstvy
E-Mail Website
Guest Editor
Department of Physics and Astronomy, University of Potsdam, Potsdam, Germany
Interests: theoretical biophysics; DNA and proteins; statistical and polymer physics; nonergodicity

Special Issue Information

Dear Colleagues, 

The adsorption of biocolloids, particularly proteins, at solid/liquid interfaces is important both in terms of their industrial preparation, purification or storage, and due to the wide spectrum of other practical applications. Especially important are the control of protein and cell separation, the production of biosensors and biochips, the transport of medicines, as well as the bio-integration of materials used for the production of medical implants.

Although deposited biomolecules play a crucial role in many processes, the mechanisms and kinetics of the immobilization processes are poorly understood. There is a lack of systematic investigations of the relationship between the deposition conditions and resulting biomolecules’ coverage, local distribution, the orientation of adsorbed particles, and their bioactivity. There is no doubt that all investigations related to biomolecule adsorption at solid substrates are desired.

This Special Issue is devoted to the most recent research focused on the determination of the mechanisms of biomolecules (proteins, peptides, biocompatible macroions) at solid substrates. Potential topics also include the application of new techniques in biomolecules deposition research and the analysis of biomolecules’ interactions with cells, viruses, etc.

Dr. Monika Wasilewska
Dr. Aneta Michna
Dr. Maria Morga
Dr. Andrey G. Cherstvy
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biomolecules is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2300 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biomolecule adsorption
  • kinetics of biomolecule adsorption
  • mechanisms of biomolecule deposition
  • interactions of biomolecules
  • techniques for the detection of biomolecules at solid substrates
  • interactions of proteins with biomolecules/biopolymers
  • protein and biomolecule systems
  • protein and biomolecule complexes

Published Papers (5 papers)

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Research

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Article
Cellular Response to Bone Morphogenetic Proteins-2 and -7 Covalently Bound to Photocrosslinked Heparin–Diazoresin Multilayer
by
Magdalena Wytrwal
,
Małgorzata Sekuła-Stryjewska
,
Agata Pomorska
,
Ewa Oclon
,
Ewa Zuba-Surma
,
Szczepan Zapotoczny
and
Krzysztof Szczubiałka
Biomolecules 2023, 13(5), 842; https://doi.org/10.3390/biom13050842 - 15 May 2023
Viewed by 433
Abstract
Despite the plethora of research that exists on recombinant human bone morphogenetic protein-2 and -7 (rhBMP-2 and rhBMP-7) and has been clinically approved, there is still a need to gain information that would allow for their more rational use in bone implantology. The [...] Read more.
Despite the plethora of research that exists on recombinant human bone morphogenetic protein-2 and -7 (rhBMP-2 and rhBMP-7) and has been clinically approved, there is still a need to gain information that would allow for their more rational use in bone implantology. The clinical application of supra-physiological dosages of these superactive molecules causes many serious adverse effects. At the cellular level, they play a role in osteogenesis and cellular adhesion, migration, and proliferation around the implant. Therefore, in this work, we investigated the role of the covalent binding of rhBMP-2 and rhBMP-7 separately and in combination with ultrathin multilayers composed of heparin and diazoresin in stem cells. In the first step, we optimized the protein deposition conditions via quartz crystal microbalance (QCM). Then, atomic force microscopy (AFM) and enzyme-linked immunosorbent assay (ELISA) were used to analyze protein–substrate interactions. The effect of the protein binding on the initial cell adhesion, migration, and short-term expression of osteogenesis markers was tested. In the presence of both proteins, cell flattening and adhesion became more prominent, resulting in limited motility. However, the early osteogenic marker expression significantly increased compared to the single protein systems. The presence of single proteins resulted in the elongation of cells, which promoted their migration activity. Full article
(This article belongs to the Special Issue Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces)
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Article
Rheology and Gelation of Hyaluronic Acid/Chitosan Coacervates
by
A. Basak Kayitmazer
,
Fatih Comert
,
Henning H. Winter
and
Phillip B. Messersmith
Biomolecules 2022, 12(12), 1817; https://doi.org/10.3390/biom12121817 - 05 Dec 2022
Viewed by 1380
Abstract
Hyaluronic acid (HA) and chitosan (CHI) are biopolyelectrolytes which are interesting for both the medical and polymer physics communities due to their biocompatibility and semi-flexibility, respectively. In this work, we demonstrate by rheology experiments that the linear viscoelasticity of HA/CHI coacervates depends strongly [...] Read more.
Hyaluronic acid (HA) and chitosan (CHI) are biopolyelectrolytes which are interesting for both the medical and polymer physics communities due to their biocompatibility and semi-flexibility, respectively. In this work, we demonstrate by rheology experiments that the linear viscoelasticity of HA/CHI coacervates depends strongly on the molecular weight of the polymers. Moduli for coacervates were found significantly higher than those of individual HA and CHI physical gels. A remarkable 1.5-fold increase in moduli was noted when catechol-conjugated HA and CHI were used instead. This was attributed to the conversion of coacervates to chemical gels by oxidation of 3,4-dihydroxyphenylalanine (DOPA) groups in HA and CHI to di-DOPA crosslinks. These rheological results put HA/CHI coacervates in the category of strong candidates as injectable tissue scaffolds or medical adhesives. Full article
(This article belongs to the Special Issue Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces)
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Article
Mimicking Pseudo-Virion Interactions with Abiotic Surfaces: Deposition of Polymer Nanoparticles with Albumin Corona
by
Małgorzata Nattich-Rak
,
Marta Sadowska
,
Maja Motyczyńska
and
Zbigniew Adamczyk
Biomolecules 2022, 12(11), 1658; https://doi.org/10.3390/biom12111658 - 08 Nov 2022
Viewed by 866
Abstract
Adsorption of human serum albumin (HSA) molecules on negatively charged polystyrene microparticles was studied using the dynamic light scattering, the electrophoretic and the solution depletion methods involving atomic force microscopy. Initially, the physicochemical characteristics of the albumin comprising the hydrodynamic diameter, the zeta [...] Read more.
Adsorption of human serum albumin (HSA) molecules on negatively charged polystyrene microparticles was studied using the dynamic light scattering, the electrophoretic and the solution depletion methods involving atomic force microscopy. Initially, the physicochemical characteristics of the albumin comprising the hydrodynamic diameter, the zeta potential and the isoelectric point were determined as a function of pH. Analogous characteristics of the polymer particles were acquired, including their size and zeta potential. The formation of albumin corona on the particles was investigated in situ by electrophoretic mobility measurements. The size, stability and electrokinetic properties of the particles with the corona were also determined. The particle diameter was equal to 125 nm, which coincides with the size of the SARS-CoV-2 virion. The isoelectric point of the particles appeared at a pH of 5. The deposition kinetics of the particles was determined by atomic force microscopy (AFM) under diffusion and by quartz microbalance (QCM) under flow conditions. It was shown that the deposition rate at a gold sensor abruptly vanished with pH following the decrease in the zeta potential of the particles. It is postulated that the acquired results can be used as useful reference systems mimicking virus adsorption on abiotic surfaces. Full article
(This article belongs to the Special Issue Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces)
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Review

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Review
Biocompatible Macroion/Growth Factor Assemblies for Medical Applications
by
Aneta Michna
,
Agata Pomorska
and
Ozlem Ozcan
Biomolecules 2023, 13(4), 609; https://doi.org/10.3390/biom13040609 - 28 Mar 2023
Viewed by 792
Abstract
Growth factors are a class of proteins that play a role in the proliferation (the increase in the number of cells resulting from cell division) and differentiation (when a cell undergoes changes in gene expression becoming a more specific type of cell) of [...] Read more.
Growth factors are a class of proteins that play a role in the proliferation (the increase in the number of cells resulting from cell division) and differentiation (when a cell undergoes changes in gene expression becoming a more specific type of cell) of cells. They can have both positive (accelerating the normal healing process) and negative effects (causing cancer) on disease progression and have potential applications in gene therapy and wound healing. However, their short half-life, low stability, and susceptibility to degradation by enzymes at body temperature make them easily degradable in vivo. To improve their effectiveness and stability, growth factors require carriers for delivery that protect them from heat, pH changes, and proteolysis. These carriers should also be able to deliver the growth factors to their intended destination. This review focuses on the current scientific literature concerning the physicochemical properties (such as biocompatibility, high affinity for binding growth factors, improved bioactivity and stability of the growth factors, protection from heat, pH changes or appropriate electric charge for growth factor attachment via electrostatic interactions) of macroions, growth factors, and macroion-growth factor assemblies, as well as their potential uses in medicine (e.g., diabetic wound healing, tissue regeneration, and cancer therapy). Specific attention is given to three types of growth factors: vascular endothelial growth factors, human fibroblast growth factors, and neurotrophins, as well as selected biocompatible synthetic macroions (obtained through standard polymerization techniques) and polysaccharides (natural macroions composed of repeating monomeric units of monosaccharides). Understanding the mechanisms by which growth factors bind to potential carriers could lead to more effective delivery methods for these proteins, which are of significant interest in the diagnosis and treatment of neurodegenerative and civilization diseases, as well as in the healing of chronic wounds. Full article
(This article belongs to the Special Issue Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces)
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Review
Interfacial Peptides as Affinity Modulating Agents of Protein-Protein Interactions
by
Pavel V. Ershov
,
Yuri V. Mezentsev
and
Alexis S. Ivanov
Biomolecules 2022, 12(1), 106; https://doi.org/10.3390/biom12010106 - 08 Jan 2022
Cited by 2 | Viewed by 2054
Abstract
The identification of disease-related protein-protein interactions (PPIs) creates objective conditions for their pharmacological modulation. The contact area (interfaces) of the vast majority of PPIs has some features, such as geometrical and biochemical complementarities, “hot spots”, as well as an extremely low mutation rate [...] Read more.
The identification of disease-related protein-protein interactions (PPIs) creates objective conditions for their pharmacological modulation. The contact area (interfaces) of the vast majority of PPIs has some features, such as geometrical and biochemical complementarities, “hot spots”, as well as an extremely low mutation rate that give us key knowledge to influence these PPIs. Exogenous regulation of PPIs is aimed at both inhibiting the assembly and/or destabilization of protein complexes. Often, the design of such modulators is associated with some specific problems in targeted delivery, cell penetration and proteolytic stability, as well as selective binding to cellular targets. Recent progress in interfacial peptide design has been achieved in solving all these difficulties and has provided a good efficiency in preclinical models (in vitro and in vivo). The most promising peptide-containing therapeutic formulations are under investigation in clinical trials. In this review, we update the current state-of-the-art in the field of interfacial peptides as potent modulators of a number of disease-related PPIs. Over the past years, the scientific interest has been focused on following clinically significant heterodimeric PPIs MDM2/p53, PD-1/PD-L1, HIF/HIF, NRF2/KEAP1, RbAp48/MTA1, HSP90/CDC37, BIRC5/CRM1, BIRC5/XIAP, YAP/TAZ–TEAD, TWEAK/FN14, Bcl-2/Bax, YY1/AKT, CD40/CD40L and MINT2/APP. Full article
(This article belongs to the Special Issue Mechanisms and Kinetics of Interactions of Biomolecules at Interfaces)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Cellular response on photocrosslinked heparin decorated with bone morphogenetic proteins-2 and -7
Authors: Magdalena Wytrwal-Sarna, Małgorzata Sekuła-Stryjewska, Agata Pomorska, Ewa Oclon, Ewa Zuba Surma, Krzysztof Szczubiałka, Szczepan Zapotoczny
Affiliation: Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Krakow, Poland

Title: Mimicking Pseudovirion Deposition at Abiotic Surfgaces: Deposition Kinetics of Polymer Nanoparticles with Albumin Core
Authors: M. Nattich-Rak; A.Pomorska; M. Sadowsak; Z.Adamczyk
Affiliation: Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30 - 239 Krakow, Poland
Abstract: no

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