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9 pages, 1701 KiB

Open AccessArticle

Spatiotemporal Variability of Trace Elements Fingerprints in Otoliths of Japanese Eel (Anguilla japonica) and Its Use in Tracing Geographic Origin

by Takaomi Arai and Shogo Kimura

Biology 2022, 11(12), 1733; https://doi.org/10.3390/biology11121733 - 29 Nov 2022

Cited by 1 | Viewed by 1522

Abstract

To secure traceability along supply chains of foodstuffs, the spatiotemporal variability of trace elements’ fingerprints (TEF) in fish otoliths provides a powerful tool to determine and discriminate the origin. Spatiotemporal variability of TEF was examined in a commercially important seafood, Japanese eel ( [...] Read more.

To secure traceability along supply chains of foodstuffs, the spatiotemporal variability of trace elements’ fingerprints (TEF) in fish otoliths provides a powerful tool to determine and discriminate the origin. Spatiotemporal variability of TEF was examined in a commercially important seafood, Japanese eel (Anguilla japonica), by means of laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS). Six elemental ratios (Na:Ca, Mg:Ca, P:Ca, K:Ca, Sr:Ca, and Ba:Ca) were determined in the otoliths of specimens originating from four aquaculture farms to examine the spatial variability and from one wild habitat over three years to examine the temporal variation. Significant temporal variation was found in Mg:Ca and Sr:Ca ratios; however, discriminant function analysis showed a lower temporal variation (50%) for the three years. Spatial variations were significant in Sr:Ca and Ba:Ca ratios, and discriminant function analysis showed high (80%) spatial variation among the four farms. Otolith TEF in the Japanese eel showed specific spatial variation among aquaculture farms but intangible temporal variation, suggesting the otolith TEF reflect each aquaculture environment. The present study shows that otolith TEF can be a reliable tool to discriminate the geographic origin of the Japanese eel. Full article

(This article belongs to the Special Issue Metals in Biology)

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12 pages, 2408 KiB

Open AccessArticle

Early Life History and Recruitment Processes of a Tropical Anguillid Eel Anguilla marmorata to the Pacific Coast, as Revealed by Otolith Sr:Ca Ratios and Microstructure

by Takaomi Arai

Biology 2022, 11(6), 803; https://doi.org/10.3390/biology11060803 - 24 May 2022

Cited by 1 | Viewed by 1634

Abstract

Recent progress in otolith microchemistry especially in strontium:calcium (Sr:Ca) ratios has revealed significant features of life histories in fishes. A catadromous eel, Anguilla marmorata, has the widest distribution among anguillid eels throughout the Indo-Pacific region. However, its dispersal and recruitment mechanisms in the [...] Read more.

Recent progress in otolith microchemistry especially in strontium:calcium (Sr:Ca) ratios has revealed significant features of life histories in fishes. A catadromous eel, Anguilla marmorata, has the widest distribution among anguillid eels throughout the Indo-Pacific region. However, its dispersal and recruitment mechanisms in the ocean are still unknown. The temporal and spatial variations of early life history characteristics in a tropical anguillid eel A. marmorata were examined by means of otolith Sr:Ca ratios and microstructure to understand the larval transport and recruitment processes to the coasts in the Pacific region. Durations of the larval stage and age at recruitment to the southern part of Japan ranged from 79 to 157 d and 113 to 192, respectively. No significant differences were found between recruitment months in those parameters. The early life characteristics such as larval duration and age at recruitment were constant throughout the recruitment period in the southern part of Japan. The early life history characteristics in combination with the oceanic current regime possibly determine the larval transportation and dispersion processes and further recruitment dynamics to the Pacific coast of A. marmorata. The present study also provides useful information on its biogeographic distribution in the species as determined by otolith Sr:Ca ratios and microstructure. Full article

(This article belongs to the Special Issue Metals in Biology)

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22 pages, 1350 KiB

Open AccessArticle

Potentially Toxic Metals in the High-Biomass Non-Hyperaccumulating Plant Amaranthus viridis: Human Health Risks and Phytoremediation Potentials

by Chee Kong Yap, Aziran Yaacob, Wen Siang Tan, Khalid Awadh Al-Mutairi, Wan Hee Cheng, Koe Wei Wong, Franklin Berandah Edward, Mohamad Saupi Ismail, Chen-Feng You, Weiyun Chew, Rosimah Nulit, Mohd Hafiz Ibrahim, Bintal Amin and Moslem Sharifinia

Biology 2022, 11(3), 389; https://doi.org/10.3390/biology11030389 - 01 Mar 2022

Cited by 6 | Viewed by 2540

Abstract

Human health risk and phytoremediation of potentially toxic metals (PTMs) in the edible vegetables have been widely discussed recently. This study aimed to determine the concentrations of four PTMs, namely Cd, Fe, Ni, and Zn) in Amaranthus viridis (leaves, stems, and roots) collected [...] Read more.

Human health risk and phytoremediation of potentially toxic metals (PTMs) in the edible vegetables have been widely discussed recently. This study aimed to determine the concentrations of four PTMs, namely Cd, Fe, Ni, and Zn) in Amaranthus viridis (leaves, stems, and roots) collected from 11 sampling sites in Peninsular Malaysia and to assess their human health risk (HHR). In general, the metal levels followed the order: roots > stems > leaves. The metal concentrations (µg/g) in the leaves of A. viridis ranged from 0.45 to 2.18 dry weight (dw) (0.05–0.26 wet weight (ww)), 74.8 to 535 dw (8.97–64.2 ww), 2.02 to 7.45 dw (0.24–0.89 ww), and 65.2 to 521 dw (7.83–62.6 ww), for Cd, Fe, Ni, and Zn, respectively. The positive relationships between the metals, the plant parts, and the geochemical factions of their habitat topsoils indicated the potential of A. viridis as a good biomonitor of Cd, Fe, and Ni pollution. With most of the values of the bioconcentration factor (BCF) > 1.0 and the transfer factor (TF) > 1.0, A. viridis was a very promising phytoextraction agent of Ni and Zn. Additionally, with most of the values of BCF > 1.0 and TF < 1.0, A. viridis was a very promising phytostabiliser of Cd and Fe. With respect to HHR, the target hazard quotients (THQ) for Cd, Fe, Pb, and Zn in the leaves of A. viridis were all below 1.00, indicating there were no non-carcinogenic risks of the four metals to consumers, including children and adults. Nevertheless, routine monitoring of PTMs in Amaranthus farms is much needed. Full article

(This article belongs to the Special Issue Metals in Biology)

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14 pages, 1881 KiB

Open AccessArticle

Computational Approach for Probing Redox Potential for Iron-Sulfur Clusters in Photosystem I

by Fedaa Ali, Medhat W. Shafaa and Muhamed Amin

Biology 2022, 11(3), 362; https://doi.org/10.3390/biology11030362 - 24 Feb 2022

Cited by 2 | Viewed by 1737

Abstract

Photosystem I is a light-driven electron transfer device. Available X-ray crystal structure from Thermosynechococcus elongatus showed that electron transfer pathways consist of two nearly symmetric branches of cofactors converging at the first iron–sulfur cluster F_X, which is followed by two terminal [...] Read more.

Photosystem I is a light-driven electron transfer device. Available X-ray crystal structure from Thermosynechococcus elongatus showed that electron transfer pathways consist of two nearly symmetric branches of cofactors converging at the first iron–sulfur cluster F_X, which is followed by two terminal iron–sulfur clusters F_A and F_B. Experiments have shown that F_X has lower oxidation potential than F_A and F_B, which facilitates the electron transfer reaction. Here, we use density functional theory and Multi-Conformer Continuum Electrostatics to explain the differences in the midpoint

E_{m}

potentials of the F_X, F_A and F_B clusters. Our calculations show that F_X has the lowest oxidation potential compared to F_A and F_B due to strong pairwise electrostatic interactions with surrounding residues. These interactions are shown to be dominated by the bridging sulfurs and cysteine ligands, which may be attributed to the shorter average bond distances between the oxidized Fe ion and ligating sulfurs for F_X compared to F_A and F_B. Moreover, the electrostatic repulsion between the 4Fe-4S clusters and the positive potential of the backbone atoms is lowest for F_X compared to both F_A and F_B. These results agree with the experimental measurements from the redox titrations of low-temperature EPR signals and of room temperature recombination kinetics. Full article

(This article belongs to the Special Issue Metals in Biology)

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10 pages, 2244 KiB

Open AccessArticle

by Mikołaj Dąbrowski, Anetta Zioła-Frankowska, Marcin Frankowski, Przemysław Daroszewski, Agnieszka Szymankiewicz-Szukała and Łukasz Kubaszewski

Biology 2022, 11(3), 344; https://doi.org/10.3390/biology11030344 - 22 Feb 2022

Cited by 1 | Viewed by 1727

Abstract

Background: The content of macroelements in bones varies with age and depends on sex. The aim of the study was to evaluate the content of macroelements and its correlation with age and sex in the femoral bone obtained during total hip arthroplasty. Methods: [...] Read more.

Background: The content of macroelements in bones varies with age and depends on sex. The aim of the study was to evaluate the content of macroelements and its correlation with age and sex in the femoral bone obtained during total hip arthroplasty. Methods: In the 86 patients, the content of macroelements (Ca, P, Mg, and Na) in the femoral head and neck (cancellous and cortical bone) was assessed by means of the inductively coupled plasma optical emission spectrometry analytical technique (ICP-OES). Results: There was a decrease in the content of macroelements in the cortical bone with age in the women in the 51–60 years (statistically significant: −0.59 for Ca, −0.65 for P) and over 70 years age groups (correlation not statistically significant: −0.29 for Ca, −0.38 for P). A significant decrease in the content of macroelements in the cortical bone was found in men over 70 years of age. Conclusions: Patterns of increased loss of macronutrients (Ca, P, and Mg) in the femoral neck (cortical bone) were demonstrated in the following patients with osteoarthritis: women aged 51–60 years and patients of both sexes over 70 years of age. Full article

(This article belongs to the Special Issue Metals in Biology)

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19 pages, 1367 KiB

Open AccessArticle

The Effect of Drying/Re-Flooding on Trace Metal, As and Se Fluxes in a Treatment Wetland: Addressing Growing Environmental Concerns

by Drew J. Hansen and Alex J. Horne

Biology 2022, 11(2), 188; https://doi.org/10.3390/biology11020188 - 25 Jan 2022

Cited by 5 | Viewed by 3086

Abstract

The retention of heavy metals in water treatment wetlands is well documented, but little understood. Fluxes to and from sediments for moderate concentrations of dissolved metals are particularly unknown. Treatment wetlands are dried out seasonally or occasionally for maintenance. The extent to which [...] Read more.

The retention of heavy metals in water treatment wetlands is well documented, but little understood. Fluxes to and from sediments for moderate concentrations of dissolved metals are particularly unknown. Treatment wetlands are dried out seasonally or occasionally for maintenance. The extent to which heavy metals may be released by drying/re-flooding is of particular concern because of the potential for toxic levels of metals to be mobilized. A 36 ha treatment wetland receiving treated oil refinery effluent in California was dried for 6 months, then re-flooded to an average depth of >10 cm. The concentrations of 11 metals, As and Se in inflow, outflow, and porewaters were measured weekly for 4 months. Mass flux rates showed that the wetland acted as a sink for As and Se, six metals (Co, Cr, Mg, Mn, Ni, and Sr) and S were overall sources and five showed zero net flux (Ba, Cu, Fe, Mo, and Zn). Porewater results indicate that oxidation of the sediments caused the source metals to be released. Removal for As > Cu, Fe, Mo, Zn > Co, Mn, Ni was consistent with the thermodynamically-predicted ‘sulfide ladder’, suggesting that available sulfide was insufficient to re-sequester the entire pool of mobile chalcophile elements. Our results suggest that less-soluble sulfide metals may be immobilized prior to more-soluble metals following drying/re-flooding in coastal systems with multiple metal contaminants. Ponding for up to several weeks, depending on the metals of concern, will facilitate metal re-immobilization within sediments before waters are released and minimize impacts downstream. Research on how to speed-up the conversion of soluble metals to their insoluble sulfides or other immobilized forms is urgently needed. Full article

(This article belongs to the Special Issue Metals in Biology)

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21 pages, 1368 KiB

Open AccessArticle

Heavy Metal Effects on Biodiversity and Stress Responses of Plants Inhabiting Contaminated Soil in Khulais, Saudi Arabia

by Emad A. Alsherif, Turki M. Al-Shaikh and Hamada AbdElgawad

Biology 2022, 11(2), 164; https://doi.org/10.3390/biology11020164 - 20 Jan 2022

Cited by 21 | Viewed by 4704

Abstract

Accumulation of heavy metals in soil is becoming an increasingly serious eco-environmental problem. Thus, investigating how plants mitigate heavy metal toxicity is necessary to reduce the associated risks. Here, we aimed to assess the bioremediation and stress defense strategies of tolerant plant species [...] Read more.

Accumulation of heavy metals in soil is becoming an increasingly serious eco-environmental problem. Thus, investigating how plants mitigate heavy metal toxicity is necessary to reduce the associated risks. Here, we aimed to assess the bioremediation and stress defense strategies of tolerant plant species grown under complex heavy metals contamination. To this end, a field study was conducted on the vegetation cover of sites with different soil pollution levels. Forty-two plant species that belong to 38 genera and 21 families were identified. The pollution had a significant impact on plant richness in the polluted sites. Out of several screened plants, Amaranthus retroflexus L. was selected because of its high relative density (16.7) and a high frequency (100%) in the most polluted sites. The selected species showed a high biological concentration factor (BCF) and low translocation factor (TF) for Cu, As and Ni. To control the heavy metal-induced oxidative damage, A. retroflexus invested in detoxification (metallothionein and phytochelatins, glutathione and glutathione-S-transferase (GST). At the organ level, oxidase damage (H₂O₂, lipid and protein peroxidation) was observed, particularly in the roots. To mitigate heavy metal oxidative stress, antioxidant mechanisms (e.g., tocopherols, glutathione, peroxidases, catalase, peroxide dismutase and ASC-GSH cycle) were upregulated, mainly in the roots. Overall, our results suggested the potentiality of A. retroflexus as a promising bioremediatory and stress-tolerant plant at the same time; moreover, defense and detoxification mechanisms were uncovered. Full article

(This article belongs to the Special Issue Metals in Biology)

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26 pages, 2343 KiB

Open AccessArticle

Assessments of the Ecological and Health Risks of Potentially Toxic Metals in the Topsoils of Different Land Uses: A Case Study in Peninsular Malaysia

by Chee Kong Yap, Weiyun Chew, Khalid Awadh Al-Mutairi, Rosimah Nulit, Mohd. Hafiz Ibrahim, Koe Wei Wong, Alireza Riyahi Bakhtiari, Moslem Sharifinia, Mohamad Saupi Ismail, Wah June Leong, Wen Siang Tan, Wan Hee Cheng, Hideo Okamura, Chen Feng You and Salman Abdo Al-Shami

Biology 2022, 11(1), 2; https://doi.org/10.3390/biology11010002 - 21 Dec 2021

Cited by 10 | Viewed by 3008

Abstract

Human activities due to different land uses are being studied widely in many countries. This study aimed to determine the ecological risks and human health risk assessments (HHRA) of Cd, Pb, Ni, Cu, and Zn in the topsoils of six land uses in [...] Read more.

Human activities due to different land uses are being studied widely in many countries. This study aimed to determine the ecological risks and human health risk assessments (HHRA) of Cd, Pb, Ni, Cu, and Zn in the topsoils of six land uses in Peninsular Malaysia. The ranges of the potentially toxic metals (PTMs) in the soils (mg/kg, dry weight) of this study were 0.24–12.43 for Cd (mean: 1.94), 4.66–2363 for Cu (mean: 228), 2576–116,344 for Fe (mean: 32,618), 2.38–75.67 for Ni (mean: 16.04), 7.22–969 for Pb (mean: 115) and 11.03–3820 for Zn (mean: 512). For the ecological risk assessments, the potential ecological risk index (PERI) for single metals indicated that the severity of pollution of the five metals decreased in the following sequence: Cd > Cu > Pb > Zn > Ni. It was found that industry, landfill, rubbish heap, and mining areas were categorized as “very high ecological risk”. For HHRA, the land uses of industry, landfill and rubbish heap were found to have higher hazard quotient (HQ) values for the three pathways (with the order: ingestion > dermal contact > inhalation ingestion) of the five metals for children and adults, when compared to the mining, plantation, and residential areas. The values for both the non-carcinogenic (Cd, Cu, Ni, and Zn), and carcinogenic risks (CR) for inhalation (Cd and Ni) obtained for children and adults in this study showed no serious adverse health impacts on their health. However, of public concern, the hazard index (HI), for Pb of children at the landfill (L-3) and the rubbish heap (RH-3) sites exceeded 1.0, indicating non-carcinogenic risk (NCR) for children. Therefore, these PERI and HHRA results provided fundamental data for PTMs pollution mitigation and environmental management in areas of different land uses in Peninsular Malaysia. Full article

(This article belongs to the Special Issue Metals in Biology)

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Review

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31 pages, 6369 KiB

Open AccessReview

Overview of Optical Biosensors for Early Cancer Detection: Fundamentals, Applications and Future Perspectives

by Mohammad Y. Azab, Mohamed Farhat O. Hameed and Salah S. A. Obayya

Biology 2023, 12(2), 232; https://doi.org/10.3390/biology12020232 - 01 Feb 2023

Cited by 14 | Viewed by 4393

Abstract

Conventional cancer detection and treatment methodologies are based on surgical, chemical and radiational processes, which are expensive, time consuming and painful. Therefore, great interest has been directed toward developing sensitive, inexpensive and rapid techniques for early cancer detection. Optical biosensors have advantages in [...] Read more.

Conventional cancer detection and treatment methodologies are based on surgical, chemical and radiational processes, which are expensive, time consuming and painful. Therefore, great interest has been directed toward developing sensitive, inexpensive and rapid techniques for early cancer detection. Optical biosensors have advantages in terms of high sensitivity and being label free with a compact size. In this review paper, the state of the art of optical biosensors for early cancer detection is presented in detail. The basic idea, sensitivity analysis, advantages and limitations of the optical biosensors are discussed. This includes optical biosensors based on plasmonic waveguides, photonic crystal fibers, slot waveguides and metamaterials. Further, the traditional optical methods, such as the colorimetric technique, optical coherence tomography, surface-enhanced Raman spectroscopy and reflectometric interference spectroscopy, are addressed. Full article

(This article belongs to the Special Issue Metals in Biology)

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33 pages, 6159 KiB

Open AccessReview

Plasmonic Biosensors: Review

by Mohga E. Hamza, Muhammad A. Othman and Mohamed A. Swillam

Biology 2022, 11(5), 621; https://doi.org/10.3390/biology11050621 - 19 Apr 2022

Cited by 23 | Viewed by 5829

Abstract

Biosensors have globally been considered as biomedical diagnostic tools required in abundant areas including the development of diseases, detection of viruses, diagnosing ecological pollution, food monitoring, and a wide range of other diagnostic and therapeutic biomedical research. Recently, the broadly emerging and promising [...] Read more.

Biosensors have globally been considered as biomedical diagnostic tools required in abundant areas including the development of diseases, detection of viruses, diagnosing ecological pollution, food monitoring, and a wide range of other diagnostic and therapeutic biomedical research. Recently, the broadly emerging and promising technique of plasmonic resonance has proven to provide label-free and highly sensitive real-time analysis when used in biosensing applications. In this review, a thorough discussion regarding the most recent techniques used in the design, fabrication, and characterization of plasmonic biosensors is conducted in addition to a comparison between those techniques with regard to their advantages and possible drawbacks when applied in different fields. Full article

(This article belongs to the Special Issue Metals in Biology)

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27 pages, 1670 KiB

Open AccessReview

Significance of Shewanella Species for the Phytoavailability and Toxicity of Arsenic—A Review

by Aminu Darma, Jianjun Yang, Peiman Zandi, Jin Liu, Katarzyna Możdżeń, Xing Xia, Ali Sani, Yihao Wang and Ewald Schnug

Biology 2022, 11(3), 472; https://doi.org/10.3390/biology11030472 - 18 Mar 2022

Cited by 7 | Viewed by 3020

Abstract

The distribution of arsenic continues due to natural and anthropogenic activities, with varying degrees of impact on plants, animals, and the entire ecosystem. Interactions between iron (Fe) oxides, bacteria, and arsenic are significantly linked to changes in the mobility, toxicity, and availability of [...] Read more.

The distribution of arsenic continues due to natural and anthropogenic activities, with varying degrees of impact on plants, animals, and the entire ecosystem. Interactions between iron (Fe) oxides, bacteria, and arsenic are significantly linked to changes in the mobility, toxicity, and availability of arsenic species in aquatic and terrestrial habitats. As a result of these changes, toxic As species become available, posing a range of threats to the entire ecosystem. This review elaborates on arsenic toxicity, the mechanisms of its bioavailability, and selected remediation strategies. The article further describes how the detoxification and methylation mechanisms used by Shewanella species could serve as a potential tool for decreasing phytoavailable As and lessening its contamination in the environment. If taken into account, this approach will provide a globally sustainable and cost-effective strategy for As remediation and more information to the literature on the unique role of this bacterial species in As remediation as opposed to conventional perception of its role as a mobiliser of As. Full article

(This article belongs to the Special Issue Metals in Biology)

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40 pages, 3809 KiB

Open AccessReview

Ecological-Health Risk Assessments of Heavy Metals (Cu, Pb, and Zn) in Aquatic Sediments from the ASEAN-5 Emerging Developing Countries: A Review and Synthesis

by Chee Kong Yap and Khalid Awadh Al-Mutairi

Biology 2022, 11(1), 7; https://doi.org/10.3390/biology11010007 - 21 Dec 2021

Cited by 16 | Viewed by 4694

Abstract

The ASEAN-5 countries (Malaysia, Indonesia, Thailand, Philippines, and Vietnam) of the Association of Southeast Asian Nations as a group is an ever-increasing major economy developmental hub in Asia besides having wealthy natural resources. However, heavy metal (HM) pollution in the region is of [...] Read more.

The ASEAN-5 countries (Malaysia, Indonesia, Thailand, Philippines, and Vietnam) of the Association of Southeast Asian Nations as a group is an ever-increasing major economy developmental hub in Asia besides having wealthy natural resources. However, heavy metal (HM) pollution in the region is of increasing environmental and public concern. This study aimed to review and compile the concentrations of Cu, Pb, and Zn in the aquatic sediments of the ASEAN-5 countries published in the literature from 1981 to February 2021. The mean values of Cu, Pb, and Zn in aquatic sediments were elevated and localized in high human activity sites and compared to the earth’s upper continental crust and reference values. Based on 176 reports from 113 publications, the ranges of concentrations (mg/kg dry weight) were 0.09–3080 for Cu, 0.37–4950 for Zn, and 0.07–2666 for Pb. The ecological risk (ER) values ranged from 0.02–1077 for Cu, 0.01–95.2 for Zn, and 0.02–784 for Pb. All reports (100%) showed the Zn ER values were categorized as being between ‘low potential ecological risk’ and ‘considerable potential ecological risk’. Almost all Cu ER values (97.7%) also showed similar ranges of the above two risk categories except for a few reports. The highest Cu level (3080 mg/kg dry weight) was reported from a mine-tailing spill in Marinduque Island of the Philippines with ‘very high ecological risk’. In addition, drainage sediments in the western part of Peninsular Malaysia were categorized as Cu ’high potential ecological risk’. Almost all reports (96%) showed Pb ER values categorized as between ‘low potential ecological risk’ and ‘moderate potential ecological risk’ except for a few reports. Six reports showed Pb ER values of ‘considerable potential ecological risk’, while one report from Semarang (Indonesia) showed Pb ER of ‘very high ecological risk’ (Pb level of 2666 mg/kg dry weight). For the ingestion and dermal contact pathways for sediments from the ASEAN-5 countries, all non-carcinogenic risk (NCR) values (HI values 1.0) for Cu, Pb, and Zn reflected no NCR. The ER and human health risk assessment of Cu, Pb, and Zn were compared in an integrative and accurate manner after we reassessed the HM data mentioned in the literature. The synthesis carried out in this review provided the basis for us to consider Cu, Pb, and Zn as being of localized elevated levels. This provided evidence for the ASEAN-5 group of countries to be considered as being a new socio-economic corridor. Beyond any reasonable doubt, an ever-increasing anthropogenic input of HMs is to be expected to a certain degree. We believe that this paper provides the most fundamental useful baseline data for the future management and sustainable development of the aquatic ecosystems in the region. Lastly, we claim that this review is currently the most up-to-date review on this topic in the literature. Full article

(This article belongs to the Special Issue Metals in Biology)

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