In Situ Detection in Microfluidic-Based Cell Culture and In Vitro Micro-Physiological Models

A special issue of Bioengineering (ISSN 2306-5354).

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 3556

Special Issue Editors

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Guest Editor
Department of Chemical Engineering, University of Massachusetts Lowell, Lowell, MA 01854, USA
Interests: biomaterials; tissue engineering; cardiovascular diseases; biomineralization; wound healing; additive manufacturing; point of care diagnostics
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Special Issue Information

Dear Colleagues,

Microfluidic-based cell culture and organ-on-a-chip (OOC) which host homo/heterogeneous cellular structures have been employed for studying the time-dependent cellular behavior and cell/tissue–xenobiotic interactions over extended periods. These miniaturized bioreactors can provide a level of control over the cell culture microenvironment that cannot be achieved under traditional culture conditions. To achieve reliable in vitro functions, it is important to maintain an “in vivo-like” cell microenvironment and the phenotypic stability in their artificial milieu during the relatively long lifespan of the in vitro model that ensure reliable, repeatable, and consistent mechanistic investigations on the same set of cells/tissue. Detection of the physiological signals ex situ, using the standard analytical tools, requires rounds of several sampling or complete compromise of the cellular structure, which makes it difficult to perform long-term investigations on the same set of cells. Therefore, the integration of analytical tools within the microfluidic system would enable improved control on the cell culture microenvironment and precise cell assays with single-cell resolution.

We are delighted to present the Special Issue entitled “In Situ Detection in Microfluidic-Based Cell Culture and In Vitro Microphysiological Models” that addresses this hot topic. This collection of articles will include the most relevant work in the integration of cell (co-)culture and monitoring tools, from state-of-the-art contributions to critical reviews which will highlight the new advances in this field and high-impact applications, including:

  • On-chip cell culture and investigation
  • Sensor integration within cell culture devices
  • In situ measurement of cellular microenvironments
  • On-chip combinatorial cell assays
  • In-line monitoring of cell cultures with optical and electrochemical sensors
  • On-chip metabolic profiling
  • On-chip immune profiling (e.g., using ELISA)
  • 3D in vitro models and analysis
  • On-chip pharmacokinetics and pharmacodynamics
  • Analyses of functional cellular activity
  • Preclinical testing of drugs in living cells/tissue/organs in vitro
  • Fluid handling and automation in cell culture systems

We aim to address the most effective way to utilize this powerful technology in the drug discovery industry as well as the technical challenges that need to be overcome. We invite researchers working in this area, from academia and industry, to submit full-length research papers, short communications, and review articles that meet the goal of this Special Issue.

Dr. Qasem Ramadan
Dr. Gulden Camci-Unal
Guest Editors

Manuscript Submission Information

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Published Papers (1 paper)

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15 pages, 18334 KiB  
Extending In-Plane Impedance Measurements from 2D to 3D Cultures: Design Considerations
by Sorel E. De Leon, Lana Cleuren, Zay Yar Oo, Paul R. Stoddart and Sally L. McArthur
Bioengineering 2021, 8(1), 11; - 13 Jan 2021
Cited by 1 | Viewed by 2828
Three-dimensional (3D) cell cultures have recently emerged as tools for biologically modelling the human body. As 3D models make their way into laboratories there is a need to develop characterisation techniques that are sensitive enough to monitor the cells in real time and [...] Read more.
Three-dimensional (3D) cell cultures have recently emerged as tools for biologically modelling the human body. As 3D models make their way into laboratories there is a need to develop characterisation techniques that are sensitive enough to monitor the cells in real time and without the need for chemical labels. Impedance spectroscopy has been shown to address both of these challenges, but there has been little research into the full impedance spectrum and how the different components of the system affect the impedance signal. Here we investigate the impedance of human fibroblast cells in 2D and 3D collagen gel cultures across a broad range of frequencies (10 Hz to 5 MHz) using a commercial well with in-plane electrodes. At low frequencies in both 2D and 3D models it was observed that protein adsorption influences the magnitude of the impedance for the cell-free samples. This effect was eliminated once cells were introduced to the systems. Cell proliferation could be monitored in 2D at intermediate frequencies (30 kHz). However, the in-plane electrodes were unable to detect any changes in the impedance at any frequency when the cells were cultured in the 3D collagen gel. The results suggest that in designing impedance measurement devices, both the nature and distribution of the cells within the 3D culture as well as the architecture of the electrodes are key variables. Full article
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